A bicistronic transgene system for genetic modification of Parthenium argentatum

Ponciano, Grisel; Dong, Ning; Chen, Grace; McMahan, Colleen

doi:10.1007/s11816-018-0478-7

Cited by 8 publications

(2 citation statements)

References 45 publications

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“…Considering such a divergence between guayule and lettuce/dandelion, along with guayule’s commercial potential to produce NR in arid regions, we conjecture that in-depth molecular studies of guayule CPTs and CBP can provide the knowledge necessary to help develop guayule as an alternative NR-producer. Guayule is transformable (Ponciano et al, 2018), and utilization of its biomass via bio-refinery process has been studied (Orts and McMahan, 2016). Isolated rubber particles from guayule have been used for biochemical studies (Cornish and Siler, 1995), but guayule CPT and CBP have not been investigated at the molecular level.…”

Section: Introductionmentioning

confidence: 99%

Molecular Studies of the Protein Complexes Involving Cis-Prenyltransferase in Guayule (Parthenium argentatum), an Alternative Rubber-Producing Plant

Lakusta

Kwon

et al. 2019

Front. Plant Sci.

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Guayule ( Parthenium argentatum ) is a perennial shrub in the Asteraceae family and synthesizes a high quality, hypoallergenic cis -1,4-polyisoprene (or natural rubber; NR). Despite its potential to be an alternative NR supplier, the enzymes for cis -polyisoprene biosynthesis have not been comprehensively studied in guayule. Recently, implications of the protein complex involving cis -prenyltransferases (CPTs) and CPT-Binding Proteins (CBPs) in NR biosynthesis were shown in lettuce and dandelion, but such protein complexes have yet to be examined in guayule. Here, we identified four guayule genes – three PaCPTs ( PaCPT1-3 ) and one PaCBP , whose protein products organize PaCPT/PaCBP complexes. Co-expression of both PaCBP and each of the PaCPTs could complemented the dolichol (a short cis -polyisoprene)-deficient yeast, whereas the individual expressions could not. Microsomes from the PaCPT/PaCBP -expressing yeast efficiently incorporated 14 C-isopentenyl diphosphate into dehydrodolichyl diphosphates; however, NR with high molecular weight could not be synthesized in in vitro assays. Furthermore, co-immunoprecipitation and split-ubiquitin yeast 2-hybrid assays using PaCPTs and PaCBP confirmed the formation of protein complexes. Of the three PaCPT s, guayule transcriptomics analysis indicated that the PaCPT3 is predominantly expressed in stem and induced by cold-stress, suggesting its involvement in NR biosynthesis. The comprehensive analyses of these PaCPTs and PaCBP here provide the foundational knowledge to generate a high NR-yielding guayule.

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Section: Introductionmentioning

confidence: 99%

Molecular Studies of the Protein Complexes Involving Cis-Prenyltransferase in Guayule (Parthenium argentatum), an Alternative Rubber-Producing Plant

Lakusta

Kwon

et al. 2019

Front. Plant Sci.

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“…Similar complexes comprised of CPT and its binding protein homolog 1 0 4 were also found in lettuce, tomato, and Arabidopsis for dehydrdolichyl diphosphate biosynthesis 1 0 5 (Brasher et al, 2015;Qu et al, 2015;Kwon et al, 2016). The CPT binding proteins, including 1 3 8 (Ponciano et al, 2018), and utilization of its biomass via bio-refinery process has been studied 1 3 9 (Orts et al, 2016). Isolated rubber particles from guayule have been used for biochemical studies 1 4 0 (Cornish and Siler, 1995), but guayule CPT and CBP have not been investigated at the molecular 1 4 1 level.…”

mentioning

confidence: 77%

Characterization of cis-prenyltransferase complexes in guayule (Parthenium argentatum), an alternative natural rubber-producing plant

Kwon

et al. 2018

Preprint

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Guayule (Parthenium argentatum) is a perennial shrub in the Asteraceae family and synthesizes a high quality, hypoallergenic cis-1,4-polyisoprene (or natural rubber; NR). Despite its potential to be an alternative NR supplier, the enzymes for cis-polyisoprene biosynthesis have not been comprehensively studied in guayule. Recently, implications of the protein complex involving cis-prenyltransferases (CPTs) and CPT-binding proteins (CBPs) in NR biosynthesis were shown in lettuce and dandelion, but such protein complexes have yet to be examined in guayule. Here we identified four guayule genes – three PaCPTs (PaCPT1-3) and one PaCBP, whose protein products form PaCPT/PaCBP complexes. Co-expression of both PaCBP and each of the PaCPTs could complemented the dolichol (a short cis-polyisoprene)-deficient yeast, whereas the individual expressions could not. Microsomes from the PaCPT/PaCBP-expressing yeast efficiently incorporated 14C-isopentenyl diphosphate into dehydrodolichyl diphosphates. Furthermore, co-immunoprecipitation and split-ubiquitin yeast 2-hybrid assays using PaCPTs and PaCBP confirmed the formation of protein complexes. Of the three PaCPTs, transcriptomics analysis indicated that the protein complex formed by PaCPT3 and PaCBP is likely to be the key component in guayule NR biosynthesis. The comprehensive analyses of these PaCPTs and PaCBP here provide the foundational knowledge to generate a high NR-yielding guayule.

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Downregulation of an allene oxide synthase gene improves photosynthetic rate and alters phytohormone homeostasis in field-grown guayule

Placido

Dierig

Cruz

et al. 2020

Industrial Crops and Products

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A bicistronic transgene system for genetic modification of Parthenium argentatum

Cited by 8 publications

References 45 publications

Molecular Studies of the Protein Complexes Involving Cis-Prenyltransferase in Guayule (Parthenium argentatum), an Alternative Rubber-Producing Plant

Molecular Studies of the Protein Complexes Involving Cis-Prenyltransferase in Guayule (Parthenium argentatum), an Alternative Rubber-Producing Plant

Characterization of cis-prenyltransferase complexes in guayule (Parthenium argentatum), an alternative natural rubber-producing plant

Downregulation of an allene oxide synthase gene improves photosynthetic rate and alters phytohormone homeostasis in field-grown guayule

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