A Bilayered Poly (Lactic-Co-Glycolic Acid) Scaffold Provides Differential Cues for the Differentiation of Dental Pulp Stem Cells

Gangolli, Riddhi; Devlin, Sean M.; Gerstenhaber, Jonathan A; Lelkes, Peter I.; Yang, Maobin

doi:10.1089/ten.tea.2018.0041

Cited by 30 publications

(35 citation statements)

References 39 publications

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“…SEM revealed that the pores on the surface of the open side were significantly larger (~45 μm) than on the closed side (1 ~ 5 μm) (Figure B,C). These values are in good agreement with our previously published data …”

Section: Resultssupporting

confidence: 93%

“…Decorated micropillars on polymeric substrate surfaces induced osteogenic differentiation of DPSCs . Specifically, the closed side of our scaffold promoted DPSCs osteogenic differentiation in the absence of osteogenic induction medium . We speculated that topographic cues, such as the lower roughness and smaller pore sizes on the closed side, affected DPSCs differentiation.…”

Section: Discussionmentioning

confidence: 87%

“…Membrane-like PLGA (75:25; Evonik Industries) scaffolds were fabricated by diffusion-induced phase separation (DIPS) technique as described previously. 26 Briefly, 12% or 20% PLGA was dissolved in dimethyl sulfoxide (DMSO, Sigma) overnight. Then, the PLGA was cast on a glass plate and submerged into deionized water, independently.…”

Section: Scaffold Fabrication and Topographic Testingmentioning

confidence: 99%

“…Importantly, the observed scaffold-guided osteogenic differentiation on the closed side occurred in basal medium, in the absence of exogenous osteogenic inducers. 26 We suppose this is likely due to the optimized topographic characteristic of our scaffold, which regulates the differentiation of DPSCs. 27 The purpose of this study was to test our hypothesis that the open and closed sides of our scaffold provide different topographic cues, which change the cytoskeleton arrangement and target the YAP signalling pathway, leading to the spontaneous differentiation of DPSCs into osteoblasts/odontoblasts on the closed side.…”

mentioning

confidence: 99%

“…25 Recognizing these limitations, our laboratory has generated a novel, bilayered biomimetic tissue scaffold that is designed to provide spatial control of dentin and pulp tissue regeneration. 26 The porous scaffold has two different layers, namely an open side and a closed side. On the open side, human dental pulp stem cells (DPSCs) penetrated into the scaffold through the channels, while on the closed side the cells rather proliferated on the surface and underwent spontaneous osteogenic differentiation.…”

mentioning

confidence: 99%

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Topographic cues of a novel bilayered scaffold modulate dental pulp stem cells differentiation by regulating YAP signalling through cytoskeleton adjustments

Montoya

Orrego

et al. 2019

Cell Proliferation

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Objectives Topographic cues can modulate morphology and differentiation of mesenchymal stem cells. This study aimed to determine how topographic cues of a novel bilayered poly (lactic‐co‐glycolic acid) (PLGA) scaffold affect osteogenic/odontogenic differentiation of dental pulp stem cells (DPSCs). Methods The surface morphology of the scaffolds was visualized by scanning electron microscope, and the surface roughness was measured by profilometry. DPSCs were cultured on each side of the scaffolds. Cell morphology, expression of Yes‐associated protein (YAP) and osteogenic/odontogenic differentiation were analysed by immunohistochemistry, real‐time polymerase chain reaction, and Alizarin Red S staining. In addition, cytochalasin D (CytoD), an F‐actin disruptor, was used to examine the effects of F‐actin on intracellular YAP localisation. Verteporfin, a YAP transcriptional inhibitor, was used to explore the effects of YAP signalling on osteogenic/odontogenic differentiation of DPSCs. Results The closed side of our scaffold showed smaller pores and less roughness than the open side. On the closed side, DPSCs exhibited enhanced F‐actin stress fibre alignment, larger spreading area, more elongated appearance, predominant nuclear YAP localization and spontaneous osteogenic differentiation. Inhibition of F‐actin alignments was correlated with nuclear YAP exclusion of DPSCs. Verteporfin restricted YAP localisation to the cytoplasm, down‐regulated expression of early osteogenic/odontogenic markers and inhibited mineralization of DPSCs cultures. Conclusions The surface topographic cues changed F‐actin alignment and morphology of DPSCs, which in turn regulated YAP signalling to control osteogenic/odontogenic differentiation.

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Section: Resultssupporting

confidence: 93%

Section: Discussionmentioning

confidence: 87%

Section: Scaffold Fabrication and Topographic Testingmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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