Which experimental models and explorations to use in regenerative endodontics? A comprehensive review on standard practices

Louvrier, Aurélien; Terranova, Lisa; Meyer, Christophe; Meyer, Florent; Euvrard, Edouard; Kroemer, Marie; Rolin, Gwenaël

doi:10.1007/s11033-021-06299-9

Cited by 5 publications

(4 citation statements)

References 86 publications

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“…The stimulation of endogenous stem cells from apical papilla or other near sources to migrate and populate root canal space in the strategy of cell homing was widely accepted as a base for modern concept of dental pulp regeneration (23) . Although cell free approach is considered more as a repair rather than true regeneration process, it is still accepted due to successful clinical outcomes as well as proven continued root development and apical foramen closure in contrary to cell based RET which is still faced by technical and ethical issues (24) .…”

Section: Discussionmentioning

confidence: 99%

Effect of Biostimulation on Response of Immature Teeth with Necrotic Pulp and Apical Periodontitis to Regenerative Endodontic Therapy in Immature Dogs Teeth

et al. 2022

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Purpose: This study was carried out to evaluate the effect of biostimulation on the regenerative response of immature teeth with necrotic pulp and apical periodontitis in dogs. Materials and Methods: Seventy-two root canals were employed in the present study as follows: Sixty root canals for experimental groups and twelve as positive and negative controls. After the induction of pulp necrosis and apical periodontitis, roots were divided into two groups, group I: Regenerative Endodontic Therapy (RET) with subsequent biostimulation (seven sessions at 808 nm diode laser at output power of 300 mW for 90 seconds) and group II: RET without biostimulation. The groups were followed up for 1, 2 and 3 months (subgroups A, B&C). The maturation of the roots was assessed both radiographically and histologically. All data were statistically analyzed.Results: the application of biostimulation in group I demonstrated marked increase in root length, thickness and decrease in apical diameter compared to group II however, it was statistically significant only in subgroup C (12.43% versus 7.66%, 33.09% versus 17.81% and 38.12% versus 23.35 % at third month) respectively (p<0.05). Furthermore, it showed histologically the highest score of vital tissue infiltration and least inflammatory scores which was statistically significant. Conclusion: Biostimulation enhanced the response of immature teeth with necrotic pulp and apical periodontitis to regenerative endodontic therapy improving root maturation.

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Section: Discussionmentioning

confidence: 99%

Effect of Biostimulation on Response of Immature Teeth with Necrotic Pulp and Apical Periodontitis to Regenerative Endodontic Therapy in Immature Dogs Teeth

et al. 2022

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“…e available body of evidence suggests the feasibility and e ectiveness of orthotopic animal models where larger animal models, such as dogs [6][7][8][9], are preferred over smaller ones like rats [10][11][12]. Dogs still represent a more reproducible and meaningful insight as their teeth pose high similarity to human anatomy [13,14]. In addition, the literature includes semi-orthotropic studies where safety, biological response, and e ectiveness could be evaluated in which the intervention of transplanting experimental samples is carried out either subcutaneously or inferior to the renal capsule [15].…”

Section: Translational Researchmentioning

confidence: 99%

Regenerative endodontics: repair or true regeneration? A narrative review

2023

OHMR

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Regenerative endodontics therapy (RET) is a pioneering approach in dentistry that aims to restore damaged dental pulp and promote tissue regeneration in non-vital teeth. It belongs to the broader field of regenerative medicine, where stem cells, scaffolds, and bioactive molecules are central components. The goal of RET is to achieve true tissue regeneration, fully restoring both the structure and function of damaged tissues, in contrast to repair, which only restores basic functionality through scar formation or tissue remodeling. Histological studies conducted in various research models, ranging from animal studies to human case studies, have provided valuable insights into the nature of tissue regeneration in RET. However, the quality of regenerated tissue can vary significantly among studies. Some research reports have described the presence of fibrous or fibrovascular tissue with limited functionality, resembling repair rather than true regeneration. This variation can be attributed to the impact of disinfection protocols on stem cell viability and differentiation potential. In conclusion, RET has the potential for both repair and true regeneration processes, with outcomes influenced by factors such as disinfection methods and the source of stem cells. Despite the remaining challenges, promising results suggest that RET could revolutionize dental pulp regeneration, especially when approaches that utilize the body\'s stem cells are optimized for clinical use. Further research and protocol refinement is essential to fully unlock the regenerative potential of this exciting field.

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“…For in vivo observation of pulp-dentin regeneration, many different animal models are used to test cell-based therapies [ 61 ]. Initial studies were conducted on small animals such as mice and rats due to their accessibility [ 46 ].…”

Section: Tooth Structurementioning

confidence: 99%