A Bioactivity‐Based Method for Screening, Identification of Lipase Inhibitors, and Clarifying the Effects of Processing Time on Lipase Inhibitory Activity of Polygonum Multiflorum

Abstract

Traditional Chinese medicine (TCM) has been used for the treatment of many complex diseases. However, the bioactive components are always undefined. In this study, a bioactivity-based method was developed and validated to screen lipase inhibitors and evaluate the effects of processing on the lipase inhibitory activity of TCM by ultrahigh performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry and fraction collector (UHPLC/Q-TOF-MS-FC). The results showed that both Polygonum multiflorum and processed P. multiflorum extracts had inhibitory effect against lipase with IC50 values of 38.84 μg/mL and 190.6 μg/mL, respectively. Stilbenes, phenolic acid, flavonoids, and anthraquinones were considered to be the potential lipase inhibitors. Eleven potential lipase inhibitors were simultaneously determined by UHPLC. Principal component analysis (PCA) was employed in exploring the effects of processing time on lipase inhibitory activity of P. multiflorum. Compared with conventional methods, a bioactivity-based method could quantitatively analyze lipase inhibitory activity of individual constituent and provide the total lipase inhibitory activity of the samples. The results demonstrated that the activity integrated UHPLC/Q-TOF-MS-FC method was an effective and powerful tool for screening and identifying lipase inhibitors from traditional Chinese medicines.

Microwave-Assisted Extraction of Phenolic Compounds From Polygonum Multiflorum Thunb. Roots

“…Their study shows that the content of gallic acid, catechin and resveratrol in the HTOD extract were 0.26, 1.57 and 0.017 mg/g, respectively. In addition, the results of Chang et al (2016) show that these compounds also existed in the HTOD extract. The content of gallic acid, catechin and resveratrol were 0.146, 0.035 and 0.192 mg/g, respectively.…”

Quantification of eight active ingredients in crude and processed radix polygoni multiflori applying miniaturized matrix solid‐phase dispersion microextraction followed by UHPLC

“…The linear gradient elution was set as following: 95–95% A over 0–1 min, 95–80% A over 1–7 min, 80–65% A over 7–10 min, 65–35% A over 10–15 min, 35–20% A over 15–18 min, 20–95% A over 18–18.1 min. The detector was set at 280 nm and the injection volume was 2 μL .…”

Influence of different processing times on the quality of Polygoni Multiflora Radix by metabolomics based on ultra high performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry

“…Aglient 6520 Q-TOF mass spectrometer (Agilent Corporation, Santa Clara, CA, USA) coupled with the Agilent 1290 UHPLC by an ESI interface was used to identify the components in PMR extract. The chromatographic and ESI-MS spectra conditions were used to separate and identify the markers and components in sample according to our previous study [17].…”

“…Compounds 2, 4, 5 and 13 were identified unambiguously by comparing with the reference compounds. They were identified as gallic acid, catechin, epicatechin and catechin gallate, respectively [17]. The component 35 was identified by extracting ion at m/z 577 from TIC.…”

“…For the detection of loss of feruloyl moiety C 10 H 8 O 3 , compound 19 was tentatively characterized as tetrahydroxystilbene-O-(feruloyl) hexoside similar to compound 20 [29]. Compound 10 was identified as polydatin according to previous research [17,26]. Compound 18 was identified as resveratrol by comparing it with the reference standard.…”