A bioceramic scaffold composed of strontium-doped three-dimensional hydroxyapatite whiskers for enhanced bone regeneration in osteoporotic defects

Zhao, Rui; Chen, Siyu; Zhao, Wanlu; Long, Yang; Yuan, Bo; Ioan, Voicu Stefan; Antoniac, Iulian Vasile; Yang, Xiao; Zhu, Xiangdong; Zhang, Xingdong

doi:10.7150/thno.40103

Cited by 80 publications

(61 citation statements)

References 49 publications

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“…Therefore, we observe that angiogenesis is essential for osteonecrosis therapy, and there exists a close relationship between VEGF and angiogenesis. Platelet endothelial cell adhesion molecule-1 (CD31) is usually found in vascular endothelial cells and can be used to evaluate angiogenesis [ 26 , 27 ]. We chose VEGF and CD31 as the marker proteins to analyze angiogenesis.…”

Section: Resultsmentioning

confidence: 99%

Biofunctionalized composite scaffold to potentiate osteoconduction, angiogenesis, and favorable metabolic microenvironment for osteonecrosis therapy

Zhu

Jiang

Zhang

et al. 2022

Bioactive Materials

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Section: Resultsmentioning

confidence: 99%

Biofunctionalized composite scaffold to potentiate osteoconduction, angiogenesis, and favorable metabolic microenvironment for osteonecrosis therapy

Zhu

Jiang

Zhang

et al. 2022

Bioactive Materials

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“…1,2 The advancement in the field of osteoporosis treatment and prevention is undeniable, however, bone fracture regeneration in osteoporotic/geriatric patients is still problematic, hence requires novel therapeutic strategies. [3][4][5] At the molecular level, the disease is triggered by an imbalance between the activity of bone-forming and resorbing cells. 6,7 In bone formation, mesenchymal stromal stem cells (MSCs), which can differentiate into preosteoblast populations and play a fundamental role in initiating tissue regeneration, represent one of the most promising therapeutic targets.…”

Section: Introductionmentioning

confidence: 99%

Novel Nanohydroxyapatite (nHAp)-Based Scaffold Doped with Iron Oxide Nanoparticles (IO), Functionalized with Small Non-Coding RNA (miR-21/124) Modulates Expression of Runt-Related Transcriptional Factor 2 and Osteopontin, Promoting Regeneration of Osteoporotic Bone in Bilateral Cranial Defects in a Senescence-Accelerated Mouse Model (SAM/P6). PART 2

Marycz¹,

Śmieszek²,

Kornicka³

et al. 2021

IJN

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Healing of osteoporotic defects is challenging and requires innovative approaches to elicit molecular mechanisms promoting osteoblasts-osteoclasts coupling and bone homeostasis. Methods: Cytocompatibility and biocompatibility of previously characterised nanocomposites, i.e Ca 5 (PO 4 ) 3 OH/Fe 3 O 4 (later called nHAp/IO) functionalised with microRNAs (nHAp/IO@miR-21/124) was tested. In vitro studies were performed using a direct coculture system of MC3T3-E1 pre-osteoblast and 4B12 pre-osteoclasts. The analysis included determination of nanocomposite influence on cultures morphology (confocal imaging), viability and metabolic activity (Alamar Blue assay). Pro-osteogenic signals were identified at mRNA, miRNA and protein level with RT-qPCR, Western blotting and immunocytochemistry. Biocompatibility of biomaterials was tested using bilateral cranial defect performed on a senescence-accelerated mouse model, ie SAM/P6 and Balb/c. The effect of biomaterial on the process of bone healing was monitored using microcomputed tomography. Results: The nanocomposites promoted survival and metabolism of bone cells, as well as enhanced functional differentiation of pre-osteoblasts MC3T3-E1 in co-cultures with pre-osteoclasts. Differentiation of MC3T3-E1 driven by nHAp/IO@miR-21/124 nanocomposite was manifested by improved extracellular matrix differentiation and up-regulation of pro-osteogenic transcripts, ie late osteogenesis markers. The nanocomposite triggered bone healing in a cranial defect model in SAM/P6 mice and was replaced by functional bone in Balb/c mice. Conclusion:This study demonstrates that the novel nanocomposite nHAp/IO can serve as a platform for therapeutic miRNA delivery. Obtained nanocomposite elicit pro-osteogenic signals, decreasing osteoclasts differentiation, simultaneously improving osteoblasts metabolism and their transition toward pre-osteocytes and bone mineralisation. The proposed scaffold can be an effective interface for in situ regeneration of osteoporotic bone, especially in elderly patients.

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“…Furthermore, when integrated into biomaterials, Sr can be administered directly into specific bone defects, which greatly reduces the necessary drug dosage and the risk of possible side effects [29,30]. It is well-known that Sr can stimulate osteoblasts and inhibit osteoclasts in vitro, while magnesium (Mg) is important to bone metabolism, stimulates new bone formation, and increases bone cell adhesion [31][32][33]. Previous studies have shown that some kind of novel bioactive glasses and bioceramics containing Sr or Mg had an enhanced osteostimulating effect on new bone formation, thus indicating Sr 2+ ions may improve the osteogenic activity of silicate ceramics [31,[34][35][36].…”

Section: Introductionmentioning

confidence: 99%

Rational design of nonstoichiometric bioceramic scaffolds via digital light processing: tuning chemical composition and pore geometry evaluation

et al. 2021

J Biol Eng

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Bioactive ceramics are promising candidates as 3D porous substrates for bone repair in bone regenerative medicine. However, they are often inefficient in clinical applications due to mismatching mechanical properties and compromised biological performances. Herein, the additional Sr dopant is hypothesized to readily adjust the mechanical and biodegradable properties of the dilute Mg-doped wollastonite bioceramic scaffolds with different pore geometries (cylindrical-, cubic-, gyroid-) by ceramic stereolithography. The results indicate that the compressive strength of Mg/Sr co-doped bioceramic scaffolds could be tuned simultaneously by the Sr dopant and pore geometry. The cylindrical-pore scaffolds exhibit strength decay with increasing Sr content, whereas the gyroid-pore scaffolds show increasing strength and Young’s modulus as the Sr concentration is increased from 0 to 5%. The ion release could also be adjusted by pore geometry in Tris buffer, and the high Sr content may trigger a faster scaffold bio-dissolution. These results demonstrate that the mechanical strengths of the bioceramic scaffolds can be controlled from the point at which their porous structures are designed. Moreover, scaffold bio-dissolution can be tuned by pore geometry and doping foreign ions. It is reasonable to consider the nonstoichiometric bioceramic scaffolds are promising for bone regeneration, especially when dealing with pathological bone defects.

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A bioceramic scaffold composed of strontium-doped three-dimensional hydroxyapatite whiskers for enhanced bone regeneration in osteoporotic defects

Cited by 80 publications

References 49 publications

Biofunctionalized composite scaffold to potentiate osteoconduction, angiogenesis, and favorable metabolic microenvironment for osteonecrosis therapy

Biofunctionalized composite scaffold to potentiate osteoconduction, angiogenesis, and favorable metabolic microenvironment for osteonecrosis therapy

Rational design of nonstoichiometric bioceramic scaffolds via digital light processing: tuning chemical composition and pore geometry evaluation

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