A Biochemical and Cellular Approach to Explore the Antiproliferative and Prodifferentiative Activity of Aloe Arborescens Leaf Extract

Abstract

Aloe arborescens Miller, belonging to the Aloe genus (Liliaceae family), is one of the main varieties of Aloe used worldwide. Although less characterized than the commonest Aloe vera, Aloe arborescens is known to be richer in beneficial phytotherapeutic, anticancer, and radio-protective properties. It is commonly used as a pharmaceutical ingredient for its effect in burn treatment and ability to increase skin wound healing properties. However, very few studies have addressed the biological effects of Aloe at molecular level. The aim of the research is to provide evidences for the antiproliferative properties of Aloe arborescens crude leaf extract using an integrated proteomic and cellular biological approach. We analysed the composition of an Aloe arborescens leaf extract by gas chromatography-mass spectrometry analysis. We found it rich in Aloe-emodin, a hydroxylanthraquinone with known antitumoral activity and in several compounds with anti-oxidant properties. Accordingly, we show that the Aloe extract has antiproliferative effects on several human transformed cell lines and exhibits prodifferentiative effects on both primary and immortalized human keratinocyte. Proteomic analysis of whole cell extracts revealed the presence of proteins with a strong antiproliferative and antimicrobial activity specifically induced in human keratinocytes by Aloe treatment supporting its application as a therapeutical agent.

Aloe-emodin suppresses esophageal cancer cell TE1 proliferation by inhibiting AKT and ERK phosphorylation

“…A previous study by the present authors also indicated that mTORC2 is a target of aloe-emodin, and aloe-emodin can strongly inhibit the AKT activation caused by PTEN loss (36). Aloe-emodin is a natural compound from aloe or Rheum palmatum (39,40). The present study aimed to determine the efficacy of aloe-emodin in the treatment of EC.…”

Chemical characterization and surface properties of a new bioemulsifier produced by Pedobacter sp. strain MCC-Z

“…All data were processed with TopSpin. 1 H NMR diffusion experiments were performed using the LED sequence with bipolar gradients [24]. The attenuation measured with this sequence is given by:…”

“…The spots were selected for mass spectral identification by the merging of images analyses. Proteins excised from the gel were reduced, alkylated, and in situ digested with trypsin as described by Di Luccia et al [23]. The peptide mixtures were analyzed using a CHIP MS 6520 QTOF equipped with a capillary 1200 HPLC system and a chip cube (Agilent Technologies).…”

“…The lipid content of Pdb-Z was determined by GC-MS analysis as described by Di Luccia et al [23]. After an alkaline digestion, the extracted mixture of species was directly analyzed by GC-MS as TMS derivates.…”

Administration ofAloe arborescenshomogenate to cattle: interaction with rumen fermentation and gut absorption of aloin

“…Conversely, aloe-emodin was not detectable in the leaves of our Aloe arborescens. The concentration of aloin and aloe-emodin may vary among different Aloe species (Hamman 2008;Di Luccia et al 2013). In addition, breeding factors, sampling and post-harvest conditions can play an important role on the chemical characteristics of Aloe homogenates (Wang et al 2010;Lucini et al 2013).…”