A Biochemical and Clinical Comparison of two Commercially Available Creatine Kinase Iso-Enzyme MB Assay Kits Suitable for use in the Routine Medical Laboratory

Canola oil (CO) is very high in healthy unsaturated fatty acids. It is higher in the omega-3 fatty acid alpha-linolenic acid than any of other oil except flaxseed oil. Because of its light flavor, high smoke point and smooth texture, canola oil is one of the most versatile cooking oils. Oil blending is one way of the most suitable technology to overcome the problem of poor stability of canola oil. It was blend with sunflower oil (SF)and palm olein (PO) to evaluate technological and nutritional qualities. This study was conducted to assess the effects of long-term intake of different fresh edible canola oils, their blends and those frying oils, selected due to their common use in human nutrition. Sixteen groups of (50-70g) albino male rats (n = 5), were fed for 90 days on different fresh and frying oils which drown from different frying cycles of potato chips up to 30 th frying. The experiment period was divided into two durations 45-days for each. Blood serum analysis for rat groups were showed; a significant increases in liver, kidney and heart functions consider to 1 st experimental duration as compared with the control group which fed on corn oil, While these functions were non-significant difference in the 2 nd experimental duration. Serum lipid profile and Atherogenic Index (AI) were recorded a significant different among all groups as compared with the control group (G1) during 1 st experimental duration. However, HDL-C was significant decreased in the 1 st duration than that in the 2 nd. Otherwise, canola oil in the diets could be increase the HDL level than that in rats group fed on corn oil. A distinct lowering in TG level was recorded among rat groups fed on diets of CO+PO (G12:G16) in the 1 st duration. Internal antioxidant of serum coenzyme Q10 was found to decline among later groups fed on frying oils 30 th (G6, G11 and G16) ranges ˂ 70mmol/ml. A blood lymphocyte percentage recorded diminution at 8% among groups which fed on fresh CO + PO (G12) and used frying CO + PO (G13, G14 and G16) oils than other groups as well as the control group G1 through 1 st duration. Histological examination for liver, kidney, heart, intestinal and testes tissue of rats were investigated. Most of groups exhibited different histological alteration, these alerts were sever along the later groups fed on 20 th and 30 th frying cycles oil. Depend on this study, canola oil and palm olein in blend can be used to improve the health status of such fried foods and their oils up to 10 th frying cycles.

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“…Myocardial infraction: it was analysed by Creatinine kinase (CK) according to the method described by Surtees et al, (1979).…”

Section: Atherogenic Lipoprotein Index (Ai) = Tc/hdl-c Ratiomentioning

confidence: 99%

Effect of Deep-Fat Frying on Canola Oil, Palmolein and Sunflower Oil Blends: B) Biologicaland Nutritional Studies

El-Reffaei

EL-Sebeay

Elghandour

et al. 2016

Journal of Food and Dairy Sciences

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Serum levels of acute phase and cardiac proteins after myocardial infarction, surgery, and infection.

Voulgari¹,

Cummins²,

Gardecki³

et al. 1982

Heart

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SUMMARY C-reactive protein and four other acute phase reactant proteins of non-cardiac origin (orosomucoid, alphal-antitrypsin, haptoglobin, and alpha2-macroglobulin) were studied serially by laser immunonephelometric assay in sera from 17 patients with myocardial infarction. A similar comparison was made in 57 patients undergoing surgery and 72 patients with acute infection. C-reactive protein was consistently the most sensitive acute phase reactant in all three conditions. After myocardial infarction, a raised serum C-reactive protein level was found on admission in four patients before a rise in creatine kinase MB isoenzyme (CK MB). The peak C-reactive protein level was reached on the third post-infarct day and it then declined over seven days with a half-life similar to myocardial tropomyosin. Serial monitoring of serum C-reactive protein, in parallel with cardiac proteins of short half-life (CK MB) and long half-life (tropomyosin), provides maximal information for diagnosis and for detecting post-infarct complications.Acute phase reactant proteins are synthesised by the liver in response to acute tissue damage. Serial estimation of these proteins, C-reactive protein in particular,' has been used to monitor the progress of a variety of clinical disorders and the recent introduction of rapid assays, using laser ixmunonephelometric,2 rate immunonephelometric,3 and immunoradiometric4 techniques, will increase their clinical application.The original observation5 that C-reactive protein could be detected in serum after myocardial infarction was followed by a number of studies using semiquantitative immunoprecipitation techniques6-9 and, later, electroimmunoprecipitation'0 and radial immunodiffusion"I assays. These studies, and a more recent immunoradiometric assay,4 indicate the potential clinical value of measuring acute phase reactants in serum after myocardial infarction. There is, however, a need to determine whether C-reactive protein estimation has advantages over other acute phase proteins for cardiac studies and to investigate the time course of its rise and fall in serum after myocardial infarction in comparison with proteins of cardiac origin. Laser immunonephelometric assays ofC-reactive protein and

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A Biochemical and Clinical Comparison of two Commercially Available Creatine Kinase Iso-Enzyme MB Assay Kits Suitable for use in the Routine Medical Laboratory

Cited by 2 publications

References 31 publications

Effect of Deep-Fat Frying on Canola Oil, Palmolein and Sunflower Oil Blends: B) Biologicaland Nutritional Studies

Effect of Deep-Fat Frying on Canola Oil, Palmolein and Sunflower Oil Blends: B) Biologicaland Nutritional Studies

Serum levels of acute phase and cardiac proteins after myocardial infarction, surgery, and infection.

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