A biofilm resistance surface yielded by grafting of antimicrobial peptides on stainless steel surface

Abstract

Anti‐biofilm formation on the surface is a severe issue in medical implants, hull surface, and food industry. Antimicrobial peptide, magainin II, was covalently bound to stainless steel surfaces through multi‐step modification. The untreated and modified samples were analyzed by SEM‐EDS, XPS, and contact angle, respectively, which indicated the peptide was immobilized on the surfaces. The antimicrobial tests of modified samples were conducted using Staphylococcus aureus and Escherichia coli, and the results revealed that peptide modified surface decreased the biofilm and bacteria quantity of stainless steel surface.

Stainless steel coated by Cu NPs via dopamine coupling for antifouling application

“…The procedure of Cu NPs surfaces preparation follows our previous study . Dopamine was dissolved into Tris‐HCl buffer solution (pH = 8.4) to prepare dopamine solution with concentration of 2 g/L.…”

Peptide‐modified stainless steel with resistance capacity of Staphylococcus aureus biofilm formation

“…The S. aureus resistance ability of peptide‐modified surfaces was tested using plate counting method as our previous work . The treated and untreated samples were placed into 24‐well plate (one sample per well), and the surfaces of samples were deposited evenly by S. aureus solution with concentration of 10 6 CFU/mL (100 μL/disc).…”

Durable polymericN-halamine functionalized stainless steel surface for improved antibacterial and anti-biofilm activity

“…In order to render the SSL surfaces antibacterial, various surface modification strategies such as deposition of biocidal metals like silver, copper, zinc and nickel, chemical vapor deposition, electroless nickel, layer-by-layer assembly of charged polyelectrolytes, sol-gel matrix of TiO 2 etc. have been employed [7][8][9][10][11][12][13]. Surface modification of SSL with natural or synthetic polymers such as antimicrobial peptides and inorganic-organic hybrid coatings of polysilsesquioxane and quaternized poly(2-(dimethyamino)ethyl methacrylate) have been reported to inhibit bacterial colonization [12,14].…”

“…have been employed [7][8][9][10][11][12][13]. Surface modification of SSL with natural or synthetic polymers such as antimicrobial peptides and inorganic-organic hybrid coatings of polysilsesquioxane and quaternized poly(2-(dimethyamino)ethyl methacrylate) have been reported to inhibit bacterial colonization [12,14]. Generally, the above mentioned approaches exhibit migration of antimicrobial agents from surfaces, and longer contact times are needed to inactivate the bacteria, which limit their practical application.…”