A biomolecule chiral interface base on BSA for electrochemical recognition of amine enantiomers

Abstract

A composite chiral interface (BSA-MB-MWCNTs) was prepared from bovine serum albumin (BSA), methylene blue (MB), and multi-walled carbon nanotubes (MWCNTs) for chiral recognition of amine enantiomers (1S, 2S)-N,N 0 -dimethyl-1,2-cyclohexanediamine and (1R, 2R)-N,N 0 -dimethyl-1,2-cyclohexanediamine. The BSA-based composite was characterized by field emission scanning electron microscopy (FESEM) and ultraviolet-visible spectroscopy (UV-Vis). The electrochemical responses towards the two enantiomers were analyzed via cyclic voltammetry (CV), electrochemical AC impedance method (EIS), and differential-pulse voltammetry (DPV). The experimental results showed that the combination of MWCNTs and BSA could effectively improve the overall identification efficiency, and the peak current displayed by the S-enantiomer is larger, indicating that the prepared chiral surface has stronger interaction with the R-enantiomer. Under optimized condition, the current value of the oxidation peak of the chiral modified electrode showed a good linear relationship towards the amine concentration in the range of 5.0 Â 10 À3 to 5.0 Â 10 À5 mmolÁL À1 . The proposed electrochemical chiral interface is easy to handle and provides a promising electrochemical sensing platform that can be used to identify chiral amine enantiomers.

Chiral recognition of tryptophan enantiomer based on the electrode modified by polyaniline adsorption bovine serum albumin complex

“…13 Chiral recognition effect of the modified electrode toward Trp enantiomer may be due to the difference in the number of the H-bonds 14 formed by threepoint interaction principle 15 between the APS-DPANI-BSA compound and tryptophan enantiomer. Compared with the studies by Xuan et al, 16 Wei et al, 17 Lu et al, 18 and Wang et al, 19 the main advantages of this work are low cost, easy preparation, excellent chiral recognition effect, outstanding stability, and exceptional reproducibility.…”

History of Cobaltabis(dicarbollide) in Potentiometry, No Need for Ionophores to Get an Excellent Selectivity

“…This unexpected result after comparing with the current techniques described earlier to differentiate enantiomers must be a consequence of the strong interactions displayed by [3,3′-Co(1,2-C 2 B 9 H 11 ) 2 ] − with the enantiomer in the membrane that prevents its fast rotation and mobility and therefore facilitates a better recognition. Recently chiral sensing systems based on chiral inorganic platforms have been reported for electrochemical recognition of enantiomers [ 64 , 65 , 66 ]. Also, [3,3′-Co(1,2-C 2 B 9 H 11 ) 2 ] − has been used in the development of ISEs for the analysis of tropane alkaloids (tropane, atropine, and scopolamine [ 67 ], the analysis of antipyrine and its metabolites/derivatives from environmental water monitoring, which are (besides their beneficial health effect) of growing concern based on their occurrence and fate in water and the environment [ 56 ] as well as for serotonin detection [ 52 ].…”

M(ii) effect on encapsulation of guests into a series of M3L2 chiral cages: enantio-recognition

“…30,31 The efficiency of chiral recognition via one of these electrochemical methods depends on the preparation of new electrodes consisting of desirable chiral materials under biological conditions. 32,33 Our group has reported various chiral coordination species for the enantiorecognition of chiral molecules. 15,17,26,31,34 However, systematic construction of a series of chiral coordination cage pairs for recognition applications remains an unexplored issue.…”