A Biphasic Approach for Characterizing Tensile, Compressive, and Hydraulic Properties of the Sclera

Abstract

Measuring the biomechanical properties of the mouse sclera is of great interest, since altered scleral properties are features of many common ocular pathologies, and the mouse is a powerful species for studying genetic factors in disease. Here, a poroelastic material model is used to analyze data from unconfined compression testing of both pig and mouse sclera, and the tensile modulus, compressive modulus, and permeability of the sclera are obtained at three levels of compressive strain. Values for all three properties measured simultaneously by unconfined compression of pig sclera were comparable to previously reported values measured by tests specific for each property, i.e., compression tests, biaxial tensile tests, and falling-head permeability assays. The repeatability of the approach was evaluated using test-retest experimental paradigm on pig sclera. Repeatability was low for measured compressive stiffness, indicating permanent changes to the samples occurring after the first test. However, reasonable repeatability for tensile stiffness and permeability was observed. The intrinsic material properties of the mouse sclera were measured for the first time. Tensile stiffness and permeability of the sclera in both species were seen to be dependent on the state of compressive strain. We conclude that unconfined compression testing of sclera, when analyzed with poroelastic theory, can be used as a powerful tool to phenotype mouse scleral changes in future genotype-phenotype association studies. Statement of SignificanceOcular biomechanics is strongly influenced by the sclera, the outermost white coat of the eye. Many ocular diseases are believed to be influenced by pathological changes to scleral microstructure and biomechanics, making intrinsic biomechanical properties an important outcome measure in many studies. However, the small mouse eye precludes the use of most traditional biomechanical characterization techniques. Here, we show that unconfined compression testing analyzed with poroelastic theory can produce measurements of biomechanical properties in the pig sclera comparable to those measured by other traditional techniques. Importantly, this technique can be successfully applied to the mouse sclera, enabling more widespread use of the species as a model for ocular disease.

Smarce1 and Tensin 4 Are Putative Modulators of Corneoscleral Stiffness

“…When based on the Step Response compliance data, mean effective tensile moduli ranged from 575 [457, 693] to 935 [800, 1,070] kPa, with a mean of 712 [693, 731] kPa. The tensile moduli of rodent cornea and sclera have been reported to range from ~2 to 5 MPa (Kling et al, 2012 ; Wang et al, 2012 ; Wu et al, 2013 ), and 0.4 to several MPa (depending on strain field) (Brown et al, 2020 ; Schwaner et al, 2020 ), respectively. Thus, our calculated effective moduli were comparable to previous reports.…”