A brainstem-to-mediodorsal thalamic pathway mediates sound-induced arousal from slow-wave sleep

Shin, Anna; Park, S.; Shin, Wooyeon; Woo, Jeonghoon; Jeong, Minju; Kim, Jeongjin; Kim, Daesoo

doi:10.1016/j.cub.2023.01.033

Cited by 13 publications

(6 citation statements)

References 45 publications

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“…CACNB2 has been implicated in psychiatric disorders 28 , 29 . The CamKIIα MD thalamic neurons (MD CamKIIα ) could act and cause arousal in mice from slow-wave sleep 59 . These findings suggesting the potential role of nasal microbiota in neuropsychiatric diseases.…”

Section: Discussionmentioning

confidence: 99%

A genome-wide association study reveals the relationship between human genetic variation and the nasal microbiome

Liu,

Tong,

Zou

et al. 2024

Commun Biol

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The nasal cavity harbors diverse microbiota that contributes to human health and respiratory diseases. However, whether and to what extent the host genome shapes the nasal microbiome remains largely unknown. Here, by dissecting the human genome and nasal metagenome data from 1401 healthy individuals, we demonstrated that the top three host genetic principal components strongly correlated with the nasal microbiota diversity and composition. The genetic association analyses identified 63 genome-wide significant loci affecting the nasal microbial taxa and functions, of which 2 loci reached study-wide significance (p < 1.7 × 10-10): rs73268759 within CAMK2A associated with genus Actinomyces and family Actinomycetaceae; and rs35211877 near POM121L12 with Gemella asaccharolytica. In addition to respiratory-related diseases, the associated loci are mainly implicated in cardiometabolic or neuropsychiatric diseases. Functional analysis showed the associated genes were most significantly expressed in the nasal airway epithelium tissue and enriched in the calcium signaling and hippo signaling pathway. Further observational correlation and Mendelian randomization analyses consistently suggested the causal effects of Serratia grimesii and Yokenella regensburgei on cardiometabolic biomarkers (cystine, glutamic acid, and creatine). This study suggested that the host genome plays an important role in shaping the nasal microbiome.

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Section: Discussionmentioning

confidence: 99%

A genome-wide association study reveals the relationship between human genetic variation and the nasal microbiome

Liu,

Tong,

Zou

et al. 2024

Commun Biol

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show abstract

“…CACNB2 has been implicated in psychiatric disorders 23,24 . The CamKIIα MD thalamic neurons (MD CamKIIα ) could act and cause arousal in mice from slow-wave sleep 51 . These ndings suggesting the potential role of nasal microbiota in neuropsychiatric diseases.…”

Section: Discussionmentioning

confidence: 99%

A genome-wide association study reveals human genetic impact on the nasal microbial diversity, taxa and functions

Zhang

Liu

Tong

et al. 2023

Preprint

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The nasal cavity harbors diverse microbiota that contribute to human health and respiratory diseases. However, whether and to what extent the host genome shapes the nasal microbiome remains largely unknown. Here, by dissecting the human genome and nasal metagenome data from 1,401 healthy individuals, we demonstrated that host genetic principal components strongly correlated with the nasal microbiota diversity and composition. The genetic association analyses identified 63 genome-wide significant loci affecting the nasal microbial taxa and functions, of which 2 loci reached study-wide significance (p < 1.7 × 10− 10): rs73268759 within CAMK2A associated with genus Actinomyces and family Actinomycetaceae; and rs35211877 near POM121L12 with Gemella asaccharolytica. In addition to respiratory-related diseases, the associated loci are mainly implicated in cardiometabolic or neuropsychiatric diseases. Functional analysis showed the associated genes were most significantly expressed in the nasal airway epithelium tissue and enriched in the calcium signaling and hippo signaling pathway. Further observational and Mendelian randomization analyses consistently revealed that several metabolites such as cystine, cystathionine, and glutamic acid play crucial roles in the host metabolism-nasal microbiota interplays. This study indicates that the contribution of the host genome to the nasal microbiome is not weaker than that of other host factors.

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“… 1 https://github.com/swyeon11/MN-MD Original codes for analysis of calcium data Shin et al. 1 https://github.com/swyeon11/MN-MD Experimental models: Organisms/strains Mice: C57BL/6J (males, 8–10 weeks) KIST animal facility Mice: Tg(Grik4-cre)G32-4Stl/J (males, 8–10 weeks) Jackson Laboratory #006474 Software and algorithms MATLAB (R2019a) MathWorks https://mathworks.com InjectoMate Neurostar https://robot-stereotaxic.com/microinjection-robot/ Doric Neuroscience Studio Doric Lenses https://neuro.doriclenses.com/products/doric-neuroscience-studio Other Stereotaxic frame Neurostar https://robot-stereotaxic.com/microinjection-robot/ Video recording system Intotech Infrared CCD camera with SmartDVR Formula software Fiber photometry system Doric Lenses Single channel two colors – GFP + RFP Optic ferrule (400 μm core diameter, 0.48NA) Doric Lenses MFC_400/430–0.48_4.0mm_ZF1.25_FLT Fiber photometry console Doric Lenses FPC LED driver Doric Lenses LEDD_2 Connectorized LED Doric Lenses CLED_465, CLED_560 Connectorized fluorescence mini-cube Doric Lenses FMC5_E1(460–490)_F1(500–540)_E2(550–580)_F2(600–680)_S Photoreceiver module Newport Co. Model 2151 <...…”

Section: Key Resources Tablementioning

confidence: 99%

“…Sleep/wake state is discriminated by video, EEG, and EMG signals. We use pClamp software (Molecular Devices) to acquire EEG and EMG signals (for details of surgery and acquisition, see Shin et al., 2023 1 ). Data analysis was performed with custom MATLAB scripts (MATLAB, The MathWorks, US).…”

Section: Quantification and Statistical Analysismentioning

confidence: 99%

Protocol for in vivo dual-color fiber photometry in the mouse thalamus

Serikov,

Martsishevska,

Shin

et al. 2024

STAR Protocols

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A brainstem-to-mediodorsal thalamic pathway mediates sound-induced arousal from slow-wave sleep

Cited by 13 publications

References 45 publications

A genome-wide association study reveals the relationship between human genetic variation and the nasal microbiome

A genome-wide association study reveals the relationship between human genetic variation and the nasal microbiome

A genome-wide association study reveals human genetic impact on the nasal microbial diversity, taxa and functions

Protocol for in vivo dual-color fiber photometry in the mouse thalamus

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