A brief history of angiogenesis assays

Cimpean, Anca Maria; Ribatti, Doménico; Raica, Marius

doi:10.1387/ijdb.103215ac

Cited by 22 publications

(23 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the in vitro assays, the three most used include rabbit cornea (Muthukkaruppan and Auerbach, 1979), the developing mouse retina (Fruttiger, 2007), and the intersegmental vessel growth in zebrafish (Lawson and Weinstein, 2002). In vitro models with isolated endothelial cell lines have allowed to study selected aspects of the angiogenic process, including endothelial migration, proliferation, proteolytic digestion of the extracellular matrix and capillary tube formation (Cimpean et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“Sprouting angiogenesis”, a reappraisal

Ribatti

Crivellato

2012

Developmental Biology

Self Cite

277

202

View full text Add to dashboard Cite

Angiogenesis is defined as a new blood vessel sprouting from pre-existing vessels. This highly regulated process take place through two non-exclusive events, the so-called endothelial sprouting or non-sprouting (intussusceptive) microvascular growth. This review article will provide a brief overview of some relevant topics defining sprouting angiogenesis and including: (i) The concept of functional specialization of endothelial cells during different phases of this process, involving the specification of endothelial cells into tip cells, stalk cells, and phalanx cells bearing different morphologies and functional properties; (ii) The interplay between numerous signaling pathways, including Notch and Notch ligands, VEGF and VEGFRs, semaphorins, and netrins, in the regulation and modulation of the phenotypic characteristics of these cells; (iii) Some fundamental and consecutive morphological processes, including lumen formation and perfusion, network formation, remodeling, pruning, leading to the final vessel maturation and stabilization.

show abstract

Section: Introductionmentioning

confidence: 99%

“Sprouting angiogenesis”, a reappraisal

Ribatti

Crivellato

2012

Developmental Biology

Self Cite

277

202

View full text Add to dashboard Cite

show abstract

“…One well‐established in vitro model for angiogenesis is the network‐forming properties of ECs on growth factor‐reduced Matrigel or similar laminin‐rich substrates: the cord‐forming assay as it was implemented in the present work allowed a complete description of EC networks formed within an entire well. Other in vitro angiogenesis models required the manual selection of imaging areas (three‐dimensional tubule assay), leading to possible experimental bias, or assayed the response of physiologically irrelevant (fetal‐ or aortic‐) endothelial cells (Cimpean et al , ). Endothelial cell cord networks are extremely dynamic, maintaining highly‐branched multicellular configurations under the influence of pro‐angiogenic factors and regressing to relatively inert cell clusters within a few hours following the removal of pro‐angiogenic factors.…”

Section: Discussionmentioning

confidence: 99%

Traumatized muscle-derived multipotent progenitor cells recruit endothelial cells through vascular endothelial growth factor-A action

Supanc

Gorman

Tuan

2017

J Tissue Eng Regen Med

View full text Add to dashboard Cite

Traumatized muscle, such as that debrided from blast injury sites, is considered a promising and convenient tissue source for multipotent progenitor cells (MPCs), a population of adult mesenchymal stem cell (MSC)-like cells. The present study aimed to assess the regenerative therapeutic potential of human traumatized muscle-derived MPCs, e.g., for injury repair in the blast-traumatized extremity, by comparing their pro-angiogenic potential in vitro and capillary recruitment activity in vivo to those of MSCs isolated from human bone marrow, a widely-used tissue source. MPCs were tested for their direct and indirect effects on human microvascular endothelial cells (ECs) in vitro. The findings reported here showed that MPC-conditioned culture medium (MPC-CM), like MSC-CM, promoted EC-cord network branching. Silent (si)RNA-mediated silencing of vascular endothelial growth factor-A (VEGF-A) expression in MPCs attenuated this effect. In a chick embryonic chorioallantoic membrane in vivo angiogenesis assay, MPCs encapsulated in photocrosslinked gelatin scaffold recruited blood vessels more efficiently than either MSCs or human foreskin fibroblasts. Together, these findings support the potential application of traumatized muscle-derived MPCs in cell-based regenerative medicine therapies as a result of their influence on EC organization. Copyright © 2017 John Wiley & Sons, Ltd.

show abstract

“…Assays confirming different aspects of reperfusion activity of CTPs can be based on existing or novel in vitro tests such as cell paracrine mechanisms, cell multiplication and proliferation, cell migration, cell differentiation or tube formation (TF) or on in vivo angiogenesis tests such as matrigel plug assay or corneal angiogenesis assay [16].…”

Section: Considerations In the Development Of Multimodal Potency Assamentioning

confidence: 99%