A brief note on the study of yieldin, a wall-bound protein that regulates the yield threshold of the cell wall

Okamoto-Nakazato, Akane

doi:10.1007/s10265-002-0040-z

Cited by 17 publications

(7 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A Ca 2+ chelator perfectly mimicked at pH 6 the acid-induced increase in wall extensibility (Ezaki et al, 2005). The reduction in the yield threshold in the pH 6–5 range was almost entirely due to a heat-sensitive component (possibly the protein “yieldin”; Okamoto-Nakazato, 2002). Instead, the acid-induced extensibility increase was comprised of a heat-sensitive (presumably expansin) and a heat-insensitive, Ca 2+ –pectate-dependent component.…”

Section: What About Diffusely Growing Cells In Land Plants?mentioning

confidence: 99%

Cell wall mechanics and growth control in plants: the role of pectins revisited

Peaucelle¹,

Braybrook²,

Höfte³

2012

Front. Plant Sci.

261

194

View full text Add to dashboard Cite

How is the extensibility of growing plant cell walls regulated? In the past, most studies have focused on the role of the cellulose/xyloglucan network and the enigmatic wall-loosening agents expansins. Here we review first how in the closest relatives of the land plants, the Charophycean algae, cell wall synthesis is coupled to cell wall extensibility by a chemical Ca2+-exchange mechanism between Ca2+–pectate complexes. We next discuss evidence for the existence in terrestrial plants of a similar “primitive” Ca2+–pectate-based growth control mechanism in parallel to the more recent, land plant-specific, expansin-dependent process.

show abstract

Section: What About Diffusely Growing Cells In Land Plants?mentioning

confidence: 99%

Cell wall mechanics and growth control in plants: the role of pectins revisited

Peaucelle¹,

Braybrook²,

Höfte³

2012

Front. Plant Sci.

261

194

View full text Add to dashboard Cite

show abstract

“…During growth, the cell wall must selectively loosen the network to accommodate volumetric expansion of the cell and withstand the expansive forces generated by cell turgor pressure. The loosening of cell walls in plants is related to: expansin (McQueenMason and Cosgrove, 1995) being able to independently induce the acid-growth of the cell wall (Cleland, 1983); numerous enzymes and proteins in the cell walls being able to alter cell wall structure (Fry, 1995;Yuan et al, 2001;Okamoto-Nakazato, 2002;Van Sandt et al, 2007); and an increased cell wall susceptibility due to the action of expansin (Cosgrove, 1999).…”

mentioning

confidence: 99%

Restoration of Mature Etiolated Cucumber Hypocotyl Cell Wall Susceptibility to Expansin by Pretreatment with Fungal Pectinases and EGTA in Vitro

et al. 2008

View full text Add to dashboard Cite

Mature plant cell walls lose their ability to expand and become unresponsive to expansin. This phenomenon is believed to be due to cross-linking of hemicellulose, pectin, or phenolic groups in the wall. By screening various hydrolytic enzymes, we found that pretreatment of nongrowing, heat-inactivated, basal cucumber (Cucumis sativus) hypocotyls with pectin lyase (Pel1) from Aspergillus japonicus could restore reconstituted exogenous expansin-induced extension in mature cell walls in vitro. Recombinant pectate lyase A (PelA) and polygalacturonase (PG) from Aspergillus spp. exhibited similar capacity to Pel1. Pel1, PelA, and PG also enhanced the reconstituted expansin-induced extension of the apical (elongating) segments of cucumber hypocotyls. However, the effective concentrations of PelA and PG for enhancing the reconstituted expansin-induced extension were greater in the apical segments than in the basal segments, whereas Pel1 behaved in the opposite manner. These data are consistent with distribution of more methyl-esterified pectin in cell walls of the apical segments and less esterified pectin in the basal segments. Associated with the degree of esterification of pectin, more calcium was found in cell walls of basal segments compared to apical segments. Pretreatment of the calcium chelator EGTA could also restore mature cell walls' susceptibility to expansin by removing calcium from mature cell walls. Because recombinant pectinases do not hydrolyze other wall polysaccharides, and endoglucanase, xylanase, and protease cannot restore the mature wall's extensibility, we can conclude that the pectin network, especially calcium-pectate bridges, may be the primary factor that determines cucumber hypocotyl mature cell walls' unresponsiveness to expansin.

show abstract

“…Expansin (Cosgrove 1997, Li et al 2003) and yieldin (Okamoto‐Nakazato 2002, Okamoto‐Nakazato et al 2000a, 2000b) have been reported as the wall‐loosening proteins, which mediate the acid growth of the plant cell wall. Expansin was isolated from growing tissue, such as cucumber hypocotyls (McQueen‐Mason et al 1992), tomato leaves (Keller and Cosgrove 1995), oat coleoptiles (Cosgrove and Li 1993, Li et al 1993) and deepwater rice (Cho and Kende 1997a, 1997b).…”

Section: Discussionmentioning

confidence: 99%

“…Yieldin was isolated from cell wall of growing cowpea hypocotyls. Yieldin caused increase in the extensibility by decreasing the yielding threshold (Okamoto‐Nakazato 2002, Okamoto‐Nakazato et al 2000a, 2000b). Immunological analysis showed that the yieldin signal was highest in the apical pre‐elongation region and became weak gradually toward the mature region (Okamoto‐Nakazato et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Role of xyloglucan in gravitropic bending of azuki bean epicotyl

Ikushima

Soga

Hoson

et al. 2008

Physiologia Plantarum

View full text Add to dashboard Cite

The mechanism of the gravitropic bending was studied in azuki bean epicotyls. The cell wall extensibility of the lower side became higher than that of the upper side in the epicotyl bending upward. The contents of matrix polysaccharides of the cell wall (pectin and xyloglucan in hemicellulose-II) in the lower side became smaller than those in the upper side. The molecular mass of xyloglucans in the lower side decreased. After an epicotyl was fixed to a metal rod to prevent the bending, gravistimulation was applied. Fundamentally the same results were obtained with respect to rheological and chemical characteristics of the cell wall as those of epicotyls showing gravitropic bending. The present results suggested that the initial gravitropic bending was caused by the increase in extensibility of the lower side and the decrease in extensibility of the upper side via the change of the cell wall matrix, especially xyloglucans.

show abstract

A brief note on the study of yieldin, a wall-bound protein that regulates the yield threshold of the cell wall

Cited by 17 publications

References 30 publications

Cell wall mechanics and growth control in plants: the role of pectins revisited

Cell wall mechanics and growth control in plants: the role of pectins revisited

Restoration of Mature Etiolated Cucumber Hypocotyl Cell Wall Susceptibility to Expansin by Pretreatment with Fungal Pectinases and EGTA in Vitro

Role of xyloglucan in gravitropic bending of azuki bean epicotyl

Contact Info

Product

Resources

About