A brief review of dispensing-based rapid prototyping techniques in tissue scaffold fabrication: role of modeling on scaffold properties prediction

Li, M G; Tian, Xiaoyu; Chen, Daniel

doi:10.1088/1758-5082/1/3/032001

Cited by 89 publications

(72 citation statements)

References 91 publications

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“…Dispensing-based cell deposition, in which pneumatic or other volumetrically driven dispensers are used to deposit the material, is considered to be a promising technique for biofabrication because of its fast and efficient material processing and manipulating capacity and has been widely employed in various tissue-engineered scaffold fabrication. However, there are various potential sources for cell damage in dispensing process, which makes the incorporation and manipulation of living cells a challenging task [1,2]. Previous studies have investigated the influence of process-induced mechanical force on cell damage/survival [3,4].…”

Section: Introductionmentioning

confidence: 99%

Influence of Calcium Ions on Cell Survival and Proliferation in the Context of an Alginate Hydrogel

Cao

Chen

Schreyer

2012

ISRN Chemical Engineering

113

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One goal of biofabrication is to incorporate living cells into artificial scaffolds in order to repair damaged tissues or organs. Although there are many studies on various biofabrication techniques, the maintenance of cell viability during the biofabrication process and cell proliferation after the process is still a challenging issue. Construction of scaffolds using hydrogels composed of natural materials can avoid exposure of cells to harsh chemicals or temperature extremes but can still entail exposure to nonphysiological conditions, causing cell damage or even death. This paper presents an experimental investigation into the influence on Schwann cell survival and proliferation of calcium used for ionic crosslinking of alginate hydrogel during the biofabrication process. The experimental results obtained show the viability and proliferation capacity of cells, either suspended in cell culture medium or encapsulated in hydrogel, and vary with the calcium concentration and the time period of cells exposed to the calcium environment. The experimental results also show the alginate concentration and cell density, that have profound influence on cell survival and proliferation, and solution viscosity as well. This study suggests the incorporation of living cells in calcium-crosslinked hydrogel in the biofabrication process can be regulated for controlled cell survival and proliferation.

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Section: Introductionmentioning

confidence: 99%

Influence of Calcium Ions on Cell Survival and Proliferation in the Context of an Alginate Hydrogel

Cao

Chen

Schreyer

2012

ISRN Chemical Engineering

113

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“…In these studies, the scaffolds with irregular internal structures were significantly simplified in the model development, thus causing to the errors in the following simulation. Currently,scaffolds manufactured by rapid prototyping (RP) techniques have shown promising in various tissue engineering applications due to their controllable microstructure [15,16] For such scaffolds, the structure is regular and the geometry can be readily modelled by CAD methods. Singh et al [17,18] utilized commercial CFD software to create models of such scaffolds in bioreactorsand studied the influence of mechanical stimuli on the velocity and shear stress distribution.…”

Section: Introductionmentioning

confidence: 99%

Modeling of the Flow within Scaffolds in Perfusion Bioreactors

Yan¹,

Chen²,

Bergstrom³

2012

AJBE

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Tissue engineering aims to produce artificial organs and tissues for transplant treatments, in which cultivating cells on scaffolds in bioreactors is of critical importance. To control the cultivating process, the knowledge of the fluid flow inside and around a scaffold in the bioreactor is essential. However, due to the complicated microstructure of a scaffold, it is difficult, or even impossible, to gain such knowledge experimentally. In contrast, numerical methods employing computational fluid dynamics (CFD) have proven promising to alleviate the problem. In this research the fluid flow in perfusion bioreactors is studied with numerical methods. The emphasis is on investigating the effect of the controllable parameters in both the scaffold fabrication (i.e., the diameter of scaffold strand and the distance between two strands) and cell culture process (i.e., the flow rate) on the distribution of shear stress within the scaffold in a perfusion bioreactor. The knowledge obtained in this study will allow for improved control strategies in scaffold fabrication and cell culturing experiments.

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“…This factor is known to affect the line width, fabrication time, and geometry resolution [19]. Our results indicate that the viscosity of the TCP paste is dependent on the shear rate and temperature to a certain extent.…”

Section: Discussionmentioning

confidence: 65%

Simulation of cortico-cancellous bone structure by 3D printing of bilayer calcium phosphate-based scaffolds

et al. 2017

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A brief review of dispensing-based rapid prototyping techniques in tissue scaffold fabrication: role of modeling on scaffold properties prediction

Cited by 89 publications

References 91 publications

Influence of Calcium Ions on Cell Survival and Proliferation in the Context of an Alginate Hydrogel

Influence of Calcium Ions on Cell Survival and Proliferation in the Context of an Alginate Hydrogel

Modeling of the Flow within Scaffolds in Perfusion Bioreactors

Simulation of cortico-cancellous bone structure by 3D printing of bilayer calcium phosphate-based scaffolds

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