A capillary electrophoresis method for identifying forensically relevant body fluids using miRNAs

Mayes, Carrie; Seashols‐Williams, Sarah; Hughes‐Stamm, Sheree

doi:10.1016/j.legalmed.2017.10.013

Cited by 33 publications

(31 citation statements)

References 29 publications

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“…We have also confirmed the usefulness of the STATH and HTN3 genes [40,41] and performed various studies to introduce the method into practice [42][43][44]. In addition, methods focusing on microRNA [45][46][47][48][49][50] and DNA methylation [51][52][53][54][55][56] have been actively explored for saliva identification. At present, it would seem that further studies are necessary before microRNA and DNA methylation methods can be applied to practical work.…”

Section: Saliva Identificationmentioning

confidence: 54%

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

2020

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Although, DNA typing plays a decisive role in the identification of persons from blood and body fluid stains in criminal investigations, clarifying the origin of extracted DNA has also been considered an essential task in proving a criminal act. This review introduces the importance of developing precise methods for body fluid identification. Body fluid identification has long relied on enzymatic methods as a presumptive assay and histological or serological methods as a confirmatory assay. However, because the latest DNA typing methods can rapidly obtain results from very small and even old, poorly preserved samples, the development of a novel corresponding body fluid identification method is required. In particular, an immunochromatographic method has been introduced to identify saliva and semen from sexual crimes. In addition, for vaginal fluid identification, attempts have been made in the past decade to introduce a method relying on body fluid-specific mRNA expression levels. At present, the development of molecular biological methods involving microRNA, DNA methylation, and resident bacterial DNA is ongoing. Therefore, in criminal investigations, body fluid identification is an essential task for correctly applying the results of DNA typing, although further research and development are required.

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Section: Saliva Identificationmentioning

confidence: 54%

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

2020

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“…Previous published studies reported promising results identifying differentially expressed miRNAs with great potential as novel biomarkers for forensic BFID (Hanson et al 2009;Zubakov et al 2010;Courts and Madea 2011;Wang et al 2013;Sauer et al 2016;Seashols-Williams et al 2016;Sirker et al 2017;Mayes et al 2018;O'Leary and Glynn 2018;Tian et al 2018). In the small number of studies published in this area, however, there is little agreement between them, with only some miRNAs suggested as potential biomarkers for specific fluids overlapping between studies (see Table 1).…”

Section: Body Fluid Identificationmentioning

confidence: 99%

“…The first to explore the forensic application of miRNAs was Erin K. Hanson in 2009(Hanson et al 2009. At first, researchers aimed to discover miRNAs that are specific to one particular body fluid; however, research studies have been unsuccessful in this search thus far (Hanson et al 2009;Zubakov et al 2010;Courts and Madea 2011;Wang et al 2013;Sauer et al 2016;Seashols-Williams et al 2016;Sirker et al 2017;Mayes et al 2018;Tian et al 2018). However, Hanson et al (2009) revealed nine miRNAs that were sufficiently differentially expressed to such a degree as to permit the identification of the particular body fluid.…”

Section: Body Fluid Identificationmentioning

confidence: 99%

Potential applications of microRNA profiling to forensic investigations

Glynn

2019

RNA

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Within the forensic science community, there is a continued push to develop novel tools to aid in criminal investigations. microRNA (miRNA) analysis has been the focus of many researcher's attention in the biomedical field since its discovery in 1993; however, the forensic application of miRNA analysis has only been suggested within the last 10 years and has been gaining considerable traction recently. The primary focus of the forensic application of miRNA analysis has been on body fluid identification to provide confirmatory universal analysis of unknown biological stains obtained from crime scenes or evidence items. There are, however, other forensic applications of miRNA profiling that have shown potential, yet are largely understudied, and warrant further investigation such as organ tissue identification, donor age estimation, and more. This review paper aims to evaluate the current literature and future potential of miRNA analysis within the forensic science field.

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“…While the stability of these markers is promising and would likely persist beyond the 180 day limit of this experiment, any alteration in expression ratios, as seen in the degraded semen samples, may yield inconclusive results using already complicated interpretation strategies via RT-qPCR methods [3,39]. CE-based miRNA assays have been developed [40][41][42]; however, issues regarding mixtures, standardization of markers, and background transcription remain.…”

Section: Discussionmentioning

confidence: 99%

The stability and persistence of blood and semen mRNA and miRNA targets for body fluid identification in environmentally challenged and laundered samples

et al. 2019

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A capillary electrophoresis method for identifying forensically relevant body fluids using miRNAs

Cited by 33 publications

References 29 publications

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

Potential applications of microRNA profiling to forensic investigations

The stability and persistence of blood and semen mRNA and miRNA targets for body fluid identification in environmentally challenged and laundered samples

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