A carbon dots-based ratiometric fluorescence probe for monitoring intracellular pH and bioimaging

Li, Songrui; Song, Xuerong; Hu, Zhiru; Feng, Guodong

doi:10.1016/j.jphotochem.2021.113129

Cited by 18 publications

(14 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The hydrodynamic diameter is bigger than that calculated by TEM, which is possibly because DLS measures the hydrodynamic diameter of the nanoparticles in solution, that is, the sum of the true size of the nanoparticles plus the solvent on their surface. 38 AuNCs were facilely prepared by employing GSH with carboxyl groups as the reducing and capping agent (Fig. 2A).…”

Section: Characterization Of Auncs and Ce@auncsmentioning

confidence: 99%

Fluorometric and colorimetric dual-mode sensing of α-glucosidase based on aggregation-induced emission enhancement of AuNCs

Li,

Zhang,

Huang

et al. 2024

J. Mater. Chem. B

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Characterization Of Auncs and Ce@auncsmentioning

confidence: 99%

Fluorometric and colorimetric dual-mode sensing of α-glucosidase based on aggregation-induced emission enhancement of AuNCs

Li,

Zhang,

Huang

et al. 2024

J. Mater. Chem. B

Self Cite

View full text Add to dashboard Cite

show abstract

“…[5][6][7][8] The versatility, spectroscopic properties, ease of modification, and high sensitivity of CDs solutions have been widely used to detect small molecules, ions, gases, and pH values. [9][10][11][12][13][14] Huang et al prepared CDs using the citric acid and alkaline magenta hydrothermal method and used them as fluorescent pH sensors. The results showed that the C─N/N─H group was successfully introduced into the CDs under UV irradiation at 380 nm, and the CDs had two fluorescence emission peaks at 475 nm and 545 nm, respectively.…”

Section: Introductionmentioning

confidence: 99%

“…Although the prepared carbon dots can meet these conditions, the application environment is complex and variable. [11,[16][17][18][19] However, the preparation of CDs that can maintain high fluorescence stability under various conditions, including extreme pH conditions such as solid acidity and alkalinity, is still challenging. In addition, the normal pH range of different tissues in an organism varies from gastric fluid (0.80-1.5) to blood (7.35-7.45) and urine (4.80-8.40).…”

Section: Introductionmentioning

confidence: 99%

One‐step Fabrication of Nitrogen‐Doped Carbon Dots‐Based Fluorescent Sensors with Red Emission for Efficient Detection of pH

Hou,

Wu,

Wang

et al. 2023

Part & Part Syst Charact

View full text Add to dashboard Cite

Carbon dots have been widely noticed due to their excellent optical properties, such as good photostability and a wide excitation wavelength range. However, the emission peaks of most known carbon dots (CDs) are in the blue region, which hinders their extensive applications in long‐wavelength emission. In this study, red‐emitting carbon dots (RCDs) that are pH‐responsive are synthesized using citric acid and urea as precursors, and their fluorescence intensity increased gradually with increasing solution pH from 1 to 12. A new mechanism based on H aggregation and Fermi energy level transfer is proposed to explain the pH‐responsive emission of RCDs. In contrast to the narrow response range of most pH‐sensitive CDs reported so far, the pH‐responsive range of RCDs is large, especially in the alkaline range, and their pronounced red light emission is well visualized. The prepared carbon dots have the advantages of non‐toxicity, low cost, and a wide pH response range. In addition, “rose” test paper and carbon dot PVA composite film, RCDs‐PVA film, are made to demonstrate the visualization of carbon dots in pH detection. The “rose” test paper and carbon dot PVA still have good fluorescence intensity. The prepared carbon dots have good application prospects for pH detection.

show abstract

“…In light of the limitations of single-channel nanoparticles, researchers are developing ratiometric sensors to improve the sensitivity of CDs to pH. (Song et al, 2017;Lei et al, 2020;Xu et al, 2020;Li et al, 2021;Silva et al, 2022). Ratiometric fluorescent sensors typically exhibit dual emission bands at different wavelengths (Silva et al, 2022).…”

Section: Graphical Abstract 1 Introductionmentioning

confidence: 99%

“…Ratiometric fluorescent sensors typically exhibit dual emission bands at different wavelengths (Silva et al, 2022). A correlation is provided from the ratios of the fluorescence intensities of the different wavelengths of the sample, which minimizes background interferences, improving the sensitivity, precision and detection of the material (Li et al, 2021). In addition, ratiometric sensors enable rapid detection due to the response of the sample, which can also be seen with the naked eye.…”

Section: Graphical Abstract 1 Introductionmentioning

confidence: 99%

Carbon dots-based fluorescent films to act as a potential antioxidant agent and pH ratiometric sensor for skin applications

Da Silva,

Araujo,

Almeida

et al. 2023

Front. Carbon

View full text Add to dashboard Cite

The wound healing process is accompanied by changes in pH values. Monitoring this physicochemical parameter can indicate the effectiveness of the applied treatment and act as early identification of wound infection. This study focuses on the development of a fluorescent film-based polyvinyl alcohol (PVA) and carbon dots (CDs) derived from lemon bagasse (CD-L) and ortho-phenylenediamine (CD-oPD) named to act as antioxidants and potential ratiometric fluorescent pH sensors, in wound applications. The I460/I550 intensity ratio, as a function of pH value for the dual-system CDs prepared from the mixture of CD-L and CD-oPD, named CD-L/oPD, was investigated. The fit corresponded to a sigmoidal function in the pH range of 5–10, with a relationship having a r2 = 0.992. The variation in the values of the I460/I550 ratio allows for the visualization of the color change from yellowish-green to green with increasing pH. Through a simulated ex vivo pig skin model, it was possible to note that the films prepared from mixed of the CD-L and CD-oPD carbon dots incorporated in a matrix PVA named CD-L/oPD-F was more efficient at visually discriminating color in relation to changes in pH than the films prepared from both individual CD-L (CD-L-F) and CD-oPD (CD-oPD-F) carbon dots. CD-L and CD-oPD demonstrated antioxidant capacity against reactive oxygen species (ROS). The IC50 values for CD-L and CD-oPD were 56.7 and 39.5 μg mL−1 in the DPPH● inhibition assay, and 25.1 and 63.4 μg mL−1 in the HOCl scavenging one, respectively. MTT viability assays using human non-tumoral skin fibroblast (HFF-1) cell showed a cell survival rate of over 80% for both CDs up to a concentration of 1,000 μg mL−1. Finally, the developed films can act in a bifunctional way, by monitoring healing through pH changes and by acting as an antioxidant agent in the treatment of wounds.

show abstract

A carbon dots-based ratiometric fluorescence probe for monitoring intracellular pH and bioimaging

Cited by 18 publications

References 42 publications

Fluorometric and colorimetric dual-mode sensing of α-glucosidase based on aggregation-induced emission enhancement of AuNCs

Fluorometric and colorimetric dual-mode sensing of α-glucosidase based on aggregation-induced emission enhancement of AuNCs

One‐step Fabrication of Nitrogen‐Doped Carbon Dots‐Based Fluorescent Sensors with Red Emission for Efficient Detection of pH

Carbon dots-based fluorescent films to act as a potential antioxidant agent and pH ratiometric sensor for skin applications

Contact Info

Product

Resources

About