A carnivorous mushroom paralyzes and kills nematodes via a volatile ketone

Lee, Ching-Han; Lee, Yi-Yun; Chang, Yian; Pon, Wen-Li; Lee, Sue-Ping; Wali, Niaz; Nakazawa, Takehito; Honda, Yoshio; Shie, Jiun‐Jie; Hsueh, Yu-Ting

doi:10.1126/sciadv.ade4809

Cited by 11 publications

(6 citation statements)

References 59 publications

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“…For example, the use of polyphenolic compounds from olive mill waste waters [32] or biosynthesized silver nanoparticles [33] as well as the activities of bacteria (Lysobacter capsici, Bacillus cereus, B. thuringiensis, Pseudomonas chlororaphis, Serratia proteamaculans, etc. [31]) and fungi [34] (Arthrobotrys oligospora, Drechslerella dactyloides [35], P. ostreatus [8,[12][13][14], etc.) are considered to be promising for nematode control.…”

Section: Discussionmentioning

confidence: 99%

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An Approach for the Control of Caenorhabditis elegans N2 via the Regulation of Growth Conditions and Pleurotus ostreatus Po4

Tashyrev,

Hovorukha,

Kudrys

et al. 2023

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Food resources are essential for the survival and growth of the population. Soil phytopathogenic nematodes cause great damage to agricultural crops, endangering food supplies and resources in general. Different methods have been used to control them. However, this issue still requires a more effective solution. Caenorhabditis elegans (CGC strain wild-type N2) was applied as a model with an Escherichia coli OP50 feeding substrate for nematodes. Our approach was based on the thermodynamically substantiated creation of growth conditions that are unfavorable for nematodes to suppress them irreversibly. The thermodynamic calculations showed that obligate anaerobic conditions, namely the absence of oxygen and a low redox potential (−100 mV and below), were potentially unacceptable for nematodes. Anaerobic conditions were created using both abiogenic (physicochemical) and biological methods. Abiogenic anaerobic conditions were achieved by preventing oxygen access and adding low-potential sodium sulfide (Eh = −250...−200 mV) to the cultivation medium. By applying biological methods, Pleurotus ostreatus Po4 and E. coli O2 was completely removed and the redox potential was decreased from +100…+200 mV to −100...−200 mV (in particular, due to the synthesis of H2S). Even the short-term exposure (1–2 days) of nematodes under anaerobic conditions led to their suppression and death. Thus, the short-term creation of anaerobic conditions in the soil may be an effective method to control, e.g., phytopathogenic aerobic nematodes. This research contributes to the development of foundations to preserve agricultural plants and increase crop yield as well as the development of an approach for the environmentally friendly control of phytopathogens.

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Section: Discussionmentioning

confidence: 99%

“…Another substance (3-octanone) produced by P. ostreatus was recently shown with the potential to kill Caenorhabditis elegans by causing the disruption of cell membrane integrity, calcium influx, etc. [14]. Thus, the use of P. ostreatus is considered to be one of the most promising methods to control nematodes.…”

Section: Introductionmentioning

confidence: 99%

An Approach for the Control of Caenorhabditis elegans N2 via the Regulation of Growth Conditions and Pleurotus ostreatus Po4

Tashyrev,

Hovorukha,

Kudrys

et al. 2023

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“…Species such as P. ostreatus, P. florida, P. eryngii, P. cornucopiae, P. sajor-caju [25], P. cystidiosus [26], and P. ostreatus strain PC9 [27] have been documented to produce specialized structures called toxocysts, which are formed on specific areas of the hypha. The toxocysts described in these reports consisted of a fine, stipe-like sterigma (1.5-4 µ m in length) protruding from the aerial hyphae and an ovoid, knob-like structure (2.5-3.5 µ m in width) [26].…”

Section: Effect Of Laccase Gene Silencing On Mycelial Ultrastructurementioning

confidence: 99%

Silencing of the Laccase (lacc2) Gene from Pleurotus ostreatus Causes Important Effects on the Formation of Toxocyst-like Structures and Fruiting Body

Armas-Tizapantzi

Pérez

Fernandez

et al. 2023

IJMS

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A wide variety of biological functions, including those involved in the morphogenesis process of basidiomycete fungi, have been attributed to laccase enzymes. In this work, RNA interference (RNAi) was used to evaluate the role of the laccase (lacc2) gene of Pleurotus ostreatus PoB. Previously, transformant strains of P. ostreatus were obtained and according to their level of silencing they were classified as light (T7), medium (T21) or severe (T26 and T27). The attenuation of the lacc2 gene in these transformants was determined by RT-PCR. Silencing of lacc2 resulted in a decrease in laccase activity between 30 and 55%, which depended on the level of laccase expression achieved. The silenced strains (T21, T26, and T27) displayed a delay in the development of mycelium on potato dextrose agar (PDA) medium, whereas in the cultures grown on wheat straw, we found that these strains were incapable of producing aerial mycelium, primordia, and fruiting bodies. Scanning electron microscopy (SEM) showed the presence of toxocyst-like structures. The highest abundance of these structures was observed in the wild-type (PoB) and T7 strains. However, the abundance of toxocysts decreased in the T21 and T26 strains, and in T27 they were not detected. These results suggest that the presence and abundance of toxocyst-like structures are directly related to the development of fruiting bodies. Furthermore, our data confirm that lacc2 is involved in the morphogenesis process of P. ostreatus.

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“…Plant pathogenic nematodes that harm important commercial crops severely restrict the development of modern agriculture, causing economic losses of $157 billion every year. − Bursaphelenchus xylophilus (B. xylophilus), Meloidogyne incognita (M.…”

Section: Introductionmentioning

confidence: 99%

Discovery and Mechanism of a Nematicide Candidate (W3): A Novel Amide Compound Containing a Cyclopropyl Moiety

Wang,

Zhang,

Wang

et al. 2024

J. Agric. Food Chem.

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To find novel nematicides, we screened the nematicidal activity of compounds in our laboratory compound library. Interestingly, the compound N-((1R,2R)-2-(2-fluoro-4-(trifluoromethyl)phenyl)cyclopropyl)-2-(trifluoromethyl)benzamide (W3) showed a broad spectrum and excellent nematicidal activity. The LC50 values of compound W3 against second-stage juveniles of Bursaphelenchus xylophilus (B. xylophilus), Aphelenchoides besseyi, and Ditylenchus destructor are 1.30, 1.63, and 0.72 mg/L, respectively. Nematicidal activities of compound W3 against second-stage juveniles of Meloidogyne incognita were 87.66% at 100 mg/L. Meanwhile, compound W3 can not only observably inhibit the feeding, reproduction, and egg hatching of B. xylophilus but can also effectively promote the oxidative stress adverse reactions of nematodes and cause intestinal damage. Compound W3 can promote the production of MDA and inhibit the activities of defense enzymes SOD and GST in B. xylophilus. Compound W3 can affect the transcription of genes involved in regulating the tricarboxylic acid cycle in nematodes, resulting in weakened nematode respiration and reduced nematode activity and even death. In addition, compound W3 had good inhibitory activity against five pathogenic fungi. Among them, the EC50 of compound W3 against Fusarium graminearum was 8.4 mg/L. In the future, we will devote ourselves to the toxicological and structural optimization research of the candidate nematicide W3.

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A carnivorous mushroom paralyzes and kills nematodes via a volatile ketone

Cited by 11 publications

References 59 publications

An Approach for the Control of Caenorhabditis elegans N2 via the Regulation of Growth Conditions and Pleurotus ostreatus Po4

An Approach for the Control of Caenorhabditis elegans N2 via the Regulation of Growth Conditions and Pleurotus ostreatus Po4

Silencing of the Laccase (lacc2) Gene from Pleurotus ostreatus Causes Important Effects on the Formation of Toxocyst-like Structures and Fruiting Body

Discovery and Mechanism of a Nematicide Candidate (W3): A Novel Amide Compound Containing a Cyclopropyl Moiety

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