1987
DOI: 10.1152/ajpgi.1987.253.5.g631
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A carrier-mediated, Na+ gradient-dependent transport for biotin in human intestinal brush-border membrane vesicles

Abstract: Transport of biotin across human intestinal brush-border membrane (BBM) was examined using brush-border membrane vesicle (BBMV) technique. Uptake of biotin by BBMV is mostly the result of transport of the substrate into an active intravesicular space with little binding to membrane surfaces. The transport of biotin was carrier mediated and was 1) Na+ (but not K+) gradient dependent with a distinct "over-shoot" phenomenon, 2) saturable as a function of concentration in the presence of a Na+ (but not a K+) gradi… Show more

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Cited by 37 publications
(52 citation statements)
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“…Bisnorbiotin and others are catabolized from biotin, and they are reported to have no activity as a co-enzyme (Zempleni et al 1997). Since these metabolites have structural similarities to biotin, the potential exists that the metabolites compete and interfere with intact active biotin for intestinal or cellular uptake, or delivery to tissues (Said et al 1987) Considering this, the patients with cramps may be in deficiency of biotin activity in spite of their apparently abundant plasma biotin levels. We infer that the total active biotin in the well-responded patients remained insufficient and therefore the prescribed biotin worked as an active vitamin.…”
Section: Patients With Cramps N=14mentioning
confidence: 99%
“…Bisnorbiotin and others are catabolized from biotin, and they are reported to have no activity as a co-enzyme (Zempleni et al 1997). Since these metabolites have structural similarities to biotin, the potential exists that the metabolites compete and interfere with intact active biotin for intestinal or cellular uptake, or delivery to tissues (Said et al 1987) Considering this, the patients with cramps may be in deficiency of biotin activity in spite of their apparently abundant plasma biotin levels. We infer that the total active biotin in the well-responded patients remained insufficient and therefore the prescribed biotin worked as an active vitamin.…”
Section: Patients With Cramps N=14mentioning
confidence: 99%
“…This was demonstrated in colorectal adenocarcinoma cells where the addition of protein kinase C activators reduced uptake of biotin whereas the presence of kinase inhibitors stimulated its uptake [60]. Primary structure analysis reveals a high degree of sequence conservation between mammalian SMVTs, with amino acid similarity ranging from 89% to 92% [64]. SMVT is widely expressed in various tissues such as intestine, liver, brain, heart, lung, kidney, cornea and placenta [70].…”
Section: Mammalian Sodium -Dependent Multivitamin Transporter (Smvt1)mentioning
confidence: 97%
“…This transporter utilises a trans-membrane sodium ion gradient and membrane potential to drive active translocation of biotin (Vitamin B7), pantothenic acid (Vitamin B5) and lipoic acid into the cell [59][60][61]. SMVT1 has been investigated using intact cells from various mammalian tissues SMVT [59,[62][63][64][65], membrane vesicles [66,67], and nonmammalian oocytes recombinantly expressing mammalian SMVT [64]. The SMVT1 is a 68,600 Da protein of 636 amino acids [64].…”
Section: Mammalian Sodium -Dependent Multivitamin Transporter (Smvt1)mentioning
confidence: 99%
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