A Case-Control Study of Acute Respiratory Tract Infection in General Practice Patients in The Netherlands

Gageldonk-Lafeber, Arianne B van; Heijnen, Marie-Louise A.; Bartelds, A.I.M.; Peters, Marcel F.; Plas, Simone M. van der; Wilbrink, B.

doi:10.1086/431982

Cited by 183 publications

(187 citation statements)

References 29 publications

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“…Compared to bacterial agents, viruses were more commonly isolated among the study subjects. These findings are in agreement with the conclusions made by van Gageldonk-Laafeber et al [2] that reported that most ARI are viral.…”

Section: Discussionsupporting

confidence: 94%

“…Parainfluenza 2 and hMPV viruses were not detected in any of the patient samples. Gageldonk-Laafeber et al [2] reported a predominant detection of Influenza and rhino viruses in a study in Netherlands. Findings of Ahmed et al [20] detected predominantly RSV (12.5%) and others were hMPV in 5.7%; Parainfluenza in 9.4%; Influenza A, 9.7%; and Influenza B in 2.6%.…”

Section: Discussionmentioning

confidence: 97%

“…Mandell [18] in another study reported Streptococcus pyogenes (11%) as the predominant bacteria in patients with ARI. A study in Netherlands by van Gageldonk-Laafeber et al [2] isolated haemolytic streptococci in 41(8%) of the study subjects. Findings of this study agree with the previous study on Streptococcus pyogenes being the most important bacterial agent associated with ARI in children.…”

Section: Discussionmentioning

confidence: 98%

“…for 58% of acute respiratory tract infections, bacteria (Group A streptococcus) 11% and 3% of patients had mixed bacterial and viral infections [2]. Various viral and bacterial strains have been associated with ARI as described below.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Aetiology of Acute Respiratory Infections in Children under Five Years in Nakuru, Kenya

Matu

Kikuvi

Wanzala

et al. 2014

JMEN

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 98%

mentioning

confidence: 99%

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Aetiology of Acute Respiratory Infections in Children under Five Years in Nakuru, Kenya

Matu

Kikuvi

Wanzala

et al. 2014

JMEN

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“…Cell-culture isolates submitted to RIVM were passaged once, and subsequently tested using PCR assays for enteroviruses (Nix et al, 2006;Oberste et al, 1999), followed by parechoviruses . Samples that remained negative for enteroviruses and parechoviruses were screened using a broad range of PCR assays for noroviruses, rotaviruses A, B and C, adenoviruses, astroviruses, sapoviruses, vesiviruses, reoviruses, a generic PCR that detects both enteroviruses and rhinoviruses, hepatitis A and E viruses, influenza A and B viruses, Aichi virus, coronaviruses 229E, NL63 and OC43, human respiratory syncytial viruses A and B and human metapneumovirus, as described previously (Svraka et al, 2007(Svraka et al, , 2009avan der Sanden et al, 2008;van Gageldonk-Lafeber et al, 2005). Samples that tested negative in all assays but showed consistent CPE were processed for metagenomic sequencing (Tables 1 and 2).…”

Section: Methodsmentioning

confidence: 99%

Metagenomic sequencing for virus identification in a public-health setting

Svraka

Rosario

Duizer

et al. 2010

Journal of General Virology

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The use of metagenomics for virus discovery in clinical samples has opened new opportunities for understanding the aetiology of unexplained illness. This study explores the potential of this sequence-independent approach in a public-health setting, by systematic analysis of samples cultured from patients with unexplained illness through a combination of PCR-based assays and viral metagenomics. In total, 1834 cell-culture isolates were collected between 1994 and 2007 through the Enterovirus Surveillance programme in the Netherlands. During the 13 year period, seven samples that exhibited reproducible cytopathogenic effects in cell culture tested negative in standard PCR assays for a range of viruses. In order to fill the diagnostic gap, viral metagenomics was applied to these culture supernatants, resulting in the rapid identification of viruses in all of the samples. The unexplained samples contained BK polyomavirus, herpes simplex virus, Newcastle disease virus and the recently discovered Saffold viruses (SAFV) (which dominated the unexplained samples; n54). The full genomic sequences of four SAFV genotype 3 (SAFV-3) viruses, which share 88-93 % nucleotide identity with known SAFV-3 viruses, are reported. Further screening for SAFV in additional cultured, unidentified clinical isolates from 2008 and 2009 resulted in identification of another SAFV-positive sample. Although the pathogenicity of the identified viruses has not been established, this study demonstrates that viral metagenomics is a powerful tool that can be integrated into public-health monitoring efforts to investigate unidentified viruses in cell cultures from clinical isolates where standard PCR assays fail to detect viruses.

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Investigation of early antibiotic use in pediatric patients with acute respiratory infections by high‐performance liquid chromatography

Toi

Doan

Minh

et al. 2019

Biomedical Chromatography

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In this study, we developed and validated two reliable high‐performance liquid chromatography (HPLC) methods for the qualitative detection of six oral β‐lactams, which are commonly used in pediatric patients with acute respiratory infections (ARIs). Two distinct reverse‐phase chromatographic separations of six β‐lactams were obtained. Four β‐lactams (cefadroxil, cephalexin, cefaclor and cefixime) in urine were separated using a gradient program with a mobile phase consisting of K2HPO4 buffer (20 mm, pH 2.8) and acetonitrile on a LichroCART 250 × 4.6 mm, Purospher STAR C18 end‐capped (5 μm) column. Two remained β‐lactams (amoxicillin and cefuroxime) were analyzed using a gradient elution with the mobile phase containing K2HPO4 buffer (20 mm, pH 3.0) and acetonitrile on a LichroCart® Purospher Star C8 end‐capped column (5 μm, 125 × 4.6 mm). Good linearity within the range of 0.3–30 μg/ml for cefadroxil, cephalexin, cefaclor and cefixime, and 0.2–20 μg/ml for amoxicillin and cefuroxime, was attained. The precisions were <14%. The accuracies ranged from 85.87 to 102.8%. The two validated methods were then applied to determine these six antibiotics in 553 urine samples of pediatric patients with ARIs. As a result, 32.2% were positive with one or more of six tested β‐lactams. Cefixime was the most commonly detected agent, accounting for 9.8% of enrolled patients.

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A Case-Control Study of Acute Respiratory Tract Infection in General Practice Patients in The Netherlands

Cited by 183 publications

References 29 publications

Aetiology of Acute Respiratory Infections in Children under Five Years in Nakuru, Kenya

Aetiology of Acute Respiratory Infections in Children under Five Years in Nakuru, Kenya

Metagenomic sequencing for virus identification in a public-health setting

Investigation of early antibiotic use in pediatric patients with acute respiratory infections by high‐performance liquid chromatography

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