A Case of Biliary Tract Infection Caused by KPC-2-Producing<i>Kluyvera ascorbata</i>

Wang, Lu; Jing, Ying; Lai, Kaisheng; An, Jingna; Yang, Jiyong

doi:10.1155/2018/5745708

Cited by 8 publications

(10 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on a report of AMR in Kluyvera -related species, bla GES-5 positive, carbapenem-resistant K. intermedia were isolated from a hospital environment ( Ribeiro et al, 2014 ). Further, KPC-2-producing K. ascorbata has been reported in a case of biliary tract infection ( Wang et al, 2018 ). A K. ascorbata isolate positive for the colistin resistance gene, mcr-1 , was identified from hospital sewage in China ( Zhao and Zong, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Complete Genome Sequence of blaIMP–6-Positive Metakosakonia sp. MRY16-398 Isolate From the Ascites of a Diverticulitis Patient

Sekizuka¹,

Matsui²,

Takahashi³

et al. 2018

Front. Microbiol.

View full text Add to dashboard Cite

A novel species of carbapenemase-producing Enterobacteriaceae (CPE) was isolated from a patient diagnosed with sigmoid colon diverticulitis. At first, laboratory testing suggested it was Klebsiella oxytoca or Pantoea sp.; however, a complete genome sequence of the isolate, MRY16-398, revealed that it could be novel species, most similar to [Kluyvera] intestini, of which taxonomic nomenclature is still under discussion. Orthologous conserved gene analysis among 42 related bacterial strains indicated that MRY16-398 was classified as the newly proposed genus Metakosakonia. Further, MRY16-398 was found to harbor the blaIMP-6 gene-positive class 1 integron (In722) in plasmid pMRY16-398_2 (IncN replicon, 47.4 kb in size). This finding implies that rare and opportunistic bacteria could be potential infectious agents. In conclusion, our results highlight the need for continuous monitoring for CPE even in nonpathogenic bacteria in the nosocomial environment.

show abstract

Section: Discussionmentioning

confidence: 99%

Complete Genome Sequence of blaIMP–6-Positive Metakosakonia sp. MRY16-398 Isolate From the Ascites of a Diverticulitis Patient

Sekizuka¹,

Matsui²,

Takahashi³

et al. 2018

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Furthermore, KPC-2-producing K. ascorbata has only been isolated once, from a rectal swab in a surveillance study in Israel 12 and once in China from a patient's biliary drainage. 13 We suggest that the carbapenemsusceptible K. ascorbata recovered in the fourth CT-guided drainage procedure could be related to a different clone or a heteroresistance event, but we cannot confirm this hypothesis. As far as we know, this is the first report of a KPC-2-carrying plasmid transference from a multidrug-resistant Klebsiella pneumoniae ST 437 to a Kluyvera ascorbata during abdominal infection.…”

mentioning

confidence: 74%

Putative horizontal transfer of carbapenem resistance betweenKlebsiella pneumoniaeandKluyvera ascorbataduring abdominal infection: A case report

Raro¹,

Lima-Morales

Barth

et al. 2019

Infect. Control Hosp. Epidemiol.

View full text Add to dashboard Cite

“…Kluyvera species have been described as opportunistic etiological microorganisms of adult clinical infections (23,24). Moreover, carbapenemase production has been scarcely reported among these isolates and only in relation to KPC-2 and GES-5 genes (25,26). For our isolates, bacterial identification to the genus level was achieved by mass spectrometry (MALDI-TOF MS) and partial 16S rRNA gene sequencing, but these methods were not conclusive at the species level.…”

Section: Discussionmentioning

confidence: 98%

First Report of an OXA-48- and CTX-M-213-Producing Kluyvera Species Clone Recovered from Patients Admitted in a University Hospital in Madrid, Spain

Hernández-García

León‐Sampedro

Pérez-Viso

et al. 2018

Antimicrob Agents Chemother

View full text Add to dashboard Cite

species other than also contribute to OXA-48 carbapenemase endemicity. We studied the emergence of an OXA-48-producing species clone, which expresses the novel CTX-M-213 enzyme, colonizing patients in our hospital. Rectal swabs from patients admitted in four wards (March 2014 to March 2016; R-GNOSIS project) were seeded onto Chromo ID-ESBL) and Chrom-CARB/OXA-48 chromogenic agar plates. Carbapenemases and extended-spectrum β-lactamases (ESBLs) were characterized (PCR, sequencing, cloning, and site-directed mutagenesis), and antibiotic susceptibility was determined. Clonal relatedness was established (XbaI pulsed-field gel electrophoresis [XbaI-PFGE]), and plasmid content was studied (transformation, S1 nuclease digestion-PFGE, SB-hybridization, restriction fragment length polymorphism [RFLP] analysis [DraI and HpaI], and PCR [incompatibility group and A,U, and A genes]). Whole-genome sequencing (WGS) (Illumina HiSeq-2500) and further bioinformatics analysis of plasmids (PLACNET and plasmidSPAdes) were performed. Patients' charts were reviewed. Six unrelated patients (median age, 75 years [range, 59 to 81 years]; 4/6 male patients) colonized with OXA-48-producing species isolates (>95% similarity of the PFGE pattern) were identified. Nosocomial acquisition was demonstrated. In two patients, OXA-48-producing species isolates coexisted with OXA-48-producing, , and The gene was located on an ∼60-kb IncL plasmid related to IncL/M-pOXA-48a and the novel gene in a conserved chromosomal region of species isolates. CTX-M-213, different from CTX-M-13 (K56E) but conferring a similar β-lactam resistance profile, was identified. Genomic analysis also revealed a 177-kb IncF plasmid (class I integron harboring and) and an 8-kb IncQ plasmid (IS- ). We describe the first plasmid in spp. and the novel chromosomal CTX-M-213 enzyme and highlight further nosocomial dissemination of through clonal lineages or plasmids related to IncL/M-pOXA-48a.

show abstract

A Case of Biliary Tract Infection Caused by KPC-2-ProducingKluyvera ascorbata

Cited by 8 publications

References 12 publications

Complete Genome Sequence of blaIMP–6-Positive Metakosakonia sp. MRY16-398 Isolate From the Ascites of a Diverticulitis Patient

Complete Genome Sequence of blaIMP–6-Positive Metakosakonia sp. MRY16-398 Isolate From the Ascites of a Diverticulitis Patient

Putative horizontal transfer of carbapenem resistance betweenKlebsiella pneumoniaeandKluyvera ascorbataduring abdominal infection: A case report

First Report of an OXA-48- and CTX-M-213-Producing Kluyvera Species Clone Recovered from Patients Admitted in a University Hospital in Madrid, Spain

Contact Info

Product

Resources

About