A case of human fasciolosis: discrepancy between egg size and genotype of Fasciola sp

Inoue, Ken; Kanemasa, Hidetoshi; Inoué, Kaori; Matsumoto, Masafumi; Kajita, Yoshihiro; Mitsufuji, Shoji; Kataoka, Keisyo; Okanoue, Takeshi; Yamada, Minoru; Uchikawa, Ryuichi; Tegoshi, Tatsuya; Arizono, Naoki

doi:10.1007/s00436-006-0370-1

Cited by 20 publications

(11 citation statements)

References 12 publications

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“…In Alexandria, Lower Egypt based on the size of the eggs, 51.3% of infections are caused by F. hepatica and 32.65% by F. gigantica but 16.32% have mixed infection (Allam, 1992). The present study agrees with Inoue et al (2007) that the size of eggs is not a suitable base for species identification, so all worm individual are identified as 68.89% F. gigantica and 30.3% F. hepatica and 0.79% was identified as a new strain. Jansen (1972) and Duewel (1982) reported unusually large-sized F. hepatica eggs in a Dutch strain from sheep, and did not accepted to use egg size in identification of Fasciola species.…”

Section: Discussionsupporting

confidence: 91%

Phenotypic description and prevalence of Fasciola species in Qena Governorate, Egypt with special reference to a new strain of Fasciola hepatica

Hussein

Khalifa

2010

Journal of King Saud University - Science

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Section: Discussionsupporting

confidence: 91%

Phenotypic description and prevalence of Fasciola species in Qena Governorate, Egypt with special reference to a new strain of Fasciola hepatica

Hussein

Khalifa

2010

Journal of King Saud University - Science

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“…isolates were located between the two species with more resemblance to F. hepatica isolates in a same clade with an intermediate form of Fasciola sp. from Japan (27). The haplotypes 2, 6, and 3 were remarkably similar to F. hepatica isolates from the northern province of Gilan (28) …”

Section: Discussionmentioning

confidence: 64%

Morphometric and Molecular Study of Fasciola Isolates from Ruminants in Iran

Akhlaghi¹,

Mohammadi²,

Ziaali³

et al. 2018

TurkiyeParazitolDerg

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Objective: The purpose of the present study was morphometric and molecular characterization of Fasciola isolates from ruminants in Iran. Methods: Flukes were collected from the livers of 54 naturally infected sheep and cattle. The proportion of body length to width (L/W) of each fresh fluke was measured using a digital caliper. We employed receiver operating characteristic (ROC) curve analysis to explore the reliability of L/W for differentiating the two species. Polymerase chain reaction (PCR)-sequencing was performed on ribosomal Internal Transcribed Spacers (ITS) and mitochondrial cytochrome c oxidase subunit 1 (cox1) genes. The sequences were then analyzed and phylogenetic relationships were investigated. Result: Forty-eight out of 54 isolates (88.9%) were identified as F. hepatica and four isolates (7.4%) as F. gigantica. All the sheep isolates were F. hepatica, while 4 out of 10 cattle were infected with F. gigantica. The morphometric study revealed an L/W ratio of 1.2 to 6.5 in Fasciola isolates with significantly higher L/W ratio in F. gigantica (p<0.00). According to the ROC curve analysis, the L/W value of 3.55 was regarded as the critical value to discriminate between the two species. Conclusions: Findings of the present study indicate the presence of both Fasciola species in southeastern Iran. The phylogenetic analysis revealed two different clades representing F. hepatica and F. gigantica. The two isolates in this study were described as Fasciola sp. The mitochondrial DNA of these isolates were similar to F. hepatica, while their ITS fragments were identical to F. gigantica.

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“…All of the DNA samples were stored at −20°C until further use. The nucleotide sequences of mitochondrial CO1 gene were amplified using the previously described methods (Itagaki et al 2005a;Inoue et al 2007) with some modification. The primer set used to amplify the CO1 fragment was Ita 8 and Ita 9 (Itagaki et al 2005a).…”

Section: Dna Extraction and Amplificationmentioning

confidence: 99%

Characterization of Fasciola hepatica genotypes from cattle and sheep in Iran using cytochrome C oxidase gene (CO1)

2011

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The present study compared the genetic variation among 19 different isolates of Fasciola hepatica from cattle and sheep in different areas of Iran using sequence data for mitochondrial DNA gene, the subunit 1 of cytochrome C oxidase gene (CO1). Four different CO1 genotypes were detected among F. hepatica isolates that showed five variable nucleotide positions (accession nos.; GQ398051, GQ398052, GQ398053, GQ398054). Nucleotide sequence variation among 19 isolates for CO1 analyzed in this study ranged from 0% to 0.98% in Iran. Among the five polymorphism sites identified in this study, only one (T to G at position 51 in 5'end of GQ175362) resulted in putative amino acid alteration of phenylalanine (TTT) to leucine (TTG) in CO1. A phylogenetic analysis of the sequence data revealed that host associations and geographic location are likely not useful markers for Fasciola genotype classification. In addition, morphological analysis showed that the ratios of body length and body width of some (n = 5) of the 19 examined F. hepatica isolates were intermediate between F. hepatica and Fasciola gigantica, representing the substantial polymorphism of the F. hepatica species and the difficulty in the accurate recognition based on morphological features. In conclusion, Iranian F. hepatica exhibited the presence of considerable genetic diversity at CO1.

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A case of human fasciolosis: discrepancy between egg size and genotype of Fasciola sp

Cited by 20 publications

References 12 publications

Phenotypic description and prevalence of Fasciola species in Qena Governorate, Egypt with special reference to a new strain of Fasciola hepatica

Phenotypic description and prevalence of Fasciola species in Qena Governorate, Egypt with special reference to a new strain of Fasciola hepatica

Morphometric and Molecular Study of Fasciola Isolates from Ruminants in Iran

Characterization of Fasciola hepatica genotypes from cattle and sheep in Iran using cytochrome C oxidase gene (CO1)

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