A case of Trypanosoma evansi in a German Shepherd dog in Vietnam

Bui, Khanh Linh; Duong, Duc Hieu; Bui, Dao Tran Anh; Nguyen, Van Long; Do, Thom; Le, Thi‐Lan; Tran, Khanh Trang

doi:10.1016/j.parint.2020.102198

Cited by 9 publications

(11 citation statements)

References 19 publications

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“…Identification of a single dog infected with the kinetoplastid pathogen T. evansi is significant, given that canine trypanosomiasis is often lethal, generating severe pathology including edema, fever, anemia, weight loss, and ocular damage, such as keratitis and corneal opacity (Bui et al., 2021; Defontis et al., 2012; Panigrahi et al., 2015; Rjeibi et al., 2015). T. evansi has a wide mammalian host range and has been reported in dogs in nearby Thailand and Vietnam previously (Huggins et al., 2019b; Bui et al., 2021). T. evansi is more frequently found infecting water buffalo, Bubalus bubalis , a species that is common around Siem Reap where our infected dog was found.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, kinetoplastid protozoans such as Leishmania infantum also pose a risk to dogs and humans (Alvar et al., 2012; Medkour et al., 2019). In Southeast Asia, Trypanosoma evansi , the cause of “surra” in ruminants, also infects dogs through biting flies or ingestion of infected raw meat to produce severe clinical signs and death (Barameechaithanun et al., 2009; Bui et al., 2021; Rjeibi et al., 2015).…”

Section: Introductionmentioning

confidence: 99%

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A multipronged next‐generation sequencing metabarcoding approach unearths hyperdiverse and abundant dog pathogen communities in Cambodia

Huggins

Colella

Koehler

et al. 2021

Transbounding Emerging Dis

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Recent surveys in Southeast Asia, including Cambodia, have identified canine vector‐borne pathogens (VBPs), including those with zoonotic potential, as highly prevalent. The lack of veterinary care alongside the close association semidomesticated dogs have with humans in the region exacerbates these zoonotic risks. Nonetheless, the number of studies investigating such pathogens and the threats they pose to dog and human health is limited. Here, we utilize a next‐generation sequencing (NGS)‐based metabarcoding protocol to conduct an assumption‐free characterization of the bacterial, apicomplexan, and kinetoplastid blood‐borne pathogens of free‐roaming dogs from across Cambodia. From 467 dogs at five field sites, 62% were infected with one of eight confirmed pathogens, comprising Anaplasma platys (32%), Ehrlichia canis (20%), Hepatozoon canis (18%), Babesia vogeli (14%), Mycoplasma haemocanis (13%), the zoonotic pathogen Bartonella clarridgeiae (3%), Candidatus Mycoplasma haematoparvum (0.2%), and Trypanosoma evansi (0.2%). Coinfections of between two and four VBPs were common with 28% of dogs found to have a mixed infection. Moreover, DNA from putatively infectious agents belonging to the bacterial family and genera Coxiella, Mycobacterium, Neisseria, Rickettsiaceae, Treponema, and two uncharacterized Mycoplasma species were identified, in addition to protozoan genera Colpodella, Parabodo, and Bodo. Using a multiple logistic regression model, the presence of ectoparasites, abnormal mucous membranes, anemia, and total protein were found as predictors of canine VBP exposure. This study represents the first time an NGS metabarcoding technique has been used to holistically detect the bacterial and protozoan hemoparasites communities of dogs through an in‐depth survey, highlighting the power of such methods to unearth a wide spectrum of pathogenic organisms in an unbiased manner.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A multipronged next‐generation sequencing metabarcoding approach unearths hyperdiverse and abundant dog pathogen communities in Cambodia

Huggins

Colella

Koehler

et al. 2021

Transbounding Emerging Dis

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“…In SEA, some cases of canine trypanosomiasis have been reported in Malaysia and Thailand ( Barameechaithanun et al., 2009 ; Rajamanickam et al., 1985 ). The presence of T. evansi has also been molecularly detected in a dog in Vietnam ( Bui et al., 2020 ). Other than dogs, T. evansi is highly prevalent in cattle and water buffaloes in SEA ( Desquesnes et al., 2009 ; Verloo et al., 2000 ).…”

Section: Vector-borne Pathogens Of Zoonotic Concernmentioning

confidence: 99%

“…(1985) Thailand Barameechaithanun et al. (2009) Vietnam Bui et al. (2020) Leishmania infantum High Dog Philippines Colella et al.…”

Section: Arthropod Vectors Of Vbp Affecting Dogs and Cats In Sea: Wha...mentioning

confidence: 99%

Canine and feline vector-borne diseases of zoonotic concern in Southeast Asia

Nguyen

Dantas‐Torres

Otranto

2021

Current Research in Parasitology & Vector-Borne Diseases

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“…The other modes of transmission include blood transfusion, feeding on fresh meat or carcasses of infected animals, or in South America, through the bite of the common vampire bat Desmodus rotundus [3]. Dogs are considered to be highly susceptible to infections, often exhibiting clinical signs such as pale mucus membranes, corneal opacity, oedema, intermittent fever, lymphadenopathy [7,8] and death (within a week to a month) in acute forms, especially in stray dogs which are not treated and sometimes even despite treatments [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Molecular detection and phylogenetic analysis of Trypanosoma evansi infections in dogs from Punjab state, India

Kaur

Singh

Kumari

et al. 2023

Preprint

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Background Trypanosoma evansi, an extracellular flagellated protozoon, is a predominant haemoparasite in tropical and sub-tropical regions of the world, mechanically transmitted by hematophagous flies. The parasite has a broad host range and dogs are considered highly susceptible to the infection. Here, we studied the application of RoTAT 1.2 PCR assay as a sensitive and specific diagnostic assay for detection of this pathogen. Methods and results The prevalence of T. evansi infection in dogs was evaluated by screening 482 blood samples collected from various districts of Punjab state, India by microscopy and PCR assay targeting RoTat 1.2 gene sequence. Microscopy revealed the prevalence of T. evansi infection as 0.21% (1/482) whereas with PCR assay 1.45% (7/482) dogs showed positivity. The kappa value statistics revealed ″fair″ agreement between PCR assay and microscopy and the data was statistically significant (P < 0.05). Assessment of various risk factors like age, breed, location, season and sex revealed non-significant correlation with prevalence of canine trypanosomosis by PCR assay except for age by microscopy (P < 0.05). The ancestral background of the field isolates of T. evansi was also studied by phylogenetic analysis of their nucleotide sequences which revealed nucleotide variation ranging from 0.00 to 1.19%. Conclusions RoTat 1.2 gene-based PCR assay proved to be highly sensitive, specific, and affordable diagnostic test for use by clinicians to save the life of our pets.

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A case of Trypanosoma evansi in a German Shepherd dog in Vietnam

Cited by 9 publications

References 19 publications

A multipronged next‐generation sequencing metabarcoding approach unearths hyperdiverse and abundant dog pathogen communities in Cambodia

A multipronged next‐generation sequencing metabarcoding approach unearths hyperdiverse and abundant dog pathogen communities in Cambodia

Canine and feline vector-borne diseases of zoonotic concern in Southeast Asia

Molecular detection and phylogenetic analysis of Trypanosoma evansi infections in dogs from Punjab state, India

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