A cDNA putatively encoding the Pc90 erythrocyte membrane antigen ofPlasmodium chabaudi

Schmitt-Wrede, Hans-Peter; Qiao, Zixu; Wunderlich, Frank

doi:10.1007/bf00931224

Cited by 6 publications

(3 citation statements)

References 8 publications

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“…This protein is the major immunogenic parasite protein localized at the host erythrocyte membrane: it is synthesized by trophozoite stages of P. chabaudi, it is exported to the host cell cytoplasm, and it finally associates with the inner surface of the plasma membrane of host erythrocytes (Wunderlich et al 1985;1988a, b, c;Lanners et al 1999). The cDNA sequence of Pc90 has been largely characterized (Schmitt-Wrede et al 1993;also Hartz et al 1993;Giraldo et al 1999).…”

Section: Resultsmentioning

confidence: 98%

Hepatic miRNA expression reprogrammed by Plasmodium chabaudi malaria

et al. 2010

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Evidence is accumulating that miRNAs are critically implicated in the outcome of diseases, but little information is available for infectious diseases. This study investigates the hepatic miRNA signature in female C57BL/6 mice infected with self-healing Plasmodium chabaudi malaria. Primary infections result in approximately 50% peak parasitemia on day 8 p.i., approximately 80% survival, and development of protective immunity. The latter is evidenced as 100% survival and 1.5% peak parasitemia upon homolog re-infections of those mice which are still alive on day 56 after primary infection. Such immune mice exhibit increased levels of IgG2a and IgG2b isotypes and still contain P. chabaudi-infected erythrocytes in their livers as revealed by light microscopy and PCR analysis. Primary infections, but not secondary infections, induce an upregulation of hepatic mRNAs encoding IL-1β, TNFα, IFNγ, NF-κB, and iNOS, and a downregulation of mRNAs for CYP7A1 and SULT2A2, respectively. Using miRXplore microarrays containing 634 mouse miRNAs in combination with quantitative RT-PCR, the liver is found to respond to primary infections with an upregulation of the three miRNA species miR-26b, MCMV-miR-M23-1-5p, and miR-1274a, and a downregulation of the 16 miRNA species miR-101b, let-7a, let-7g, miR-193a-3p, miR-192, miR-142-5p, miR-465d, miR-677, miR-98, miR-694, miR-374(*), miR-450b-5p, miR-464, miR-377, miR-20a(*), and miR-466d-3p, respectively. Surprisingly, about the same pattern of miRNA expression is revealed in immune mice, and this pattern is even sustained upon homolog re-infections of immune mice. These data suggest that development of protective immunity against malarial blood stages of P. chabaudi is associated with a reprogramming of the expression of distinct miRNA species in the female mouse liver.

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Section: Resultsmentioning

confidence: 98%

Hepatic miRNA expression reprogrammed by Plasmodium chabaudi malaria

et al. 2010

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“…Genes 11w (42889–44401) and 12w (46271–47911) situated next to each other have a confirmed six‐exon structure in common, with the first exon containing a signal sequence (Table 1). Protein sequence alignments (data not shown) suggest that they may be variants of one gene family that includes the genes encoding the Pc93 kDa protein (Giraldo et al ., 1998) and Pc90 antigen (Schmitt‐Wrede et al ., 1993). A probe derived from 11w hybridized to at least 21 P. chabaudi subtelomeric fragments (Fig.…”

Section: Resultsmentioning

confidence: 99%

Ten families of variant genes encoded in subtelomeric regions of multiple chromosomes of Plasmodium chabaudi, a malaria species that undergoes antigenic variation in the laboratory mouse

Fischer

Chavchich

Huestis

et al. 2003

Molecular Microbiology

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SummaryThe chromosome ends of human malaria parasites harbour many genes encoding proteins that are exported to the surface of infected red cells, often being involved in host-parasite interactions and immune evasion. Unlike other murine malaria parasites Plasmodium chabaudi undergoes antigenic variation during passage in the laboratory mouse and hence is a model suitable for investigation of switching mechanisms. However, little is known about the subtelomeric regions of P. chabaudi chromosomes and its variable antigens. Here we report 80 kb of sequence from an end of one P. chabaudi chromosome. Hybridization of probes spanning this region to two dimensional pulsed field gels of the genome revealed 10 multicopy gene families located exclusively in subtelomeric regions of multiple P. chabaudi chromosomes, interspersed amongst multicopy intergenic regions. Hence all chromosomes share a common subtelomeric structure, presumably playing a similar role in spatial positioning as the P. falciparum Rep20 sequence. Expression in blood stages, domains characteristic of surface antigens and copy numbers between four and several hundred per genome, indicate a functional role in antigenic variation for some of these families. We identify members of the cir family, as well as novel genes, that although clearly homologous to cir have large low complexity regions in the predicted extracellular domains. Although all families have homologues in other rodent Plasmodium species, four were previously not known to be subtelomeric. Six have homologues in human and simian malarias.

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“…Several investigators have independently identi®ed proteins referred to as Pch105/RESA, Pc96, and Pc90 which are now known to be homologues of Pc(em)93 (Holmquist et al 1990;Wiser et al 1997). Cloning and sequencing the Pc(em)93 gene from three dierent P. chabaudi lines revealed tandem repeats rich in glutamic acid residues (Schmitt-Wrede et al 1993;Wanidworanun et al 1997;Giraldo et al 1998). Although quite similar, the sequences of the tandem repeats vary between parasite lines.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Plasmodium chabaudi gene encoding a protein with glutamate-rich tandem repeats

Giraldo¹,

Jennings²,

Deleersnijder

et al. 1999

Parasitology Research

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Several highly antigenic proteins containing tandem repeats rich in glutamic acid residues have been described in Plasmodium falciparum. However, relatively little information is available about analogous genes in rodent parasites. This report describes a 4.2-kb genomic DNA fragment from P. chabaudi with a deduced amino acid sequence that is predominantly glutamate-rich tandem repeats. Several dierent monoclonal antibodies raised against a 93-kDa P. chabaudi protein, which does not correspond to the cloned DNA fragment, recognize a recombinant protein expressed from the 4.2-kb DNA fragment. The only sequence similarities between these two genes are tandem repeats with a predominance of glutamate pairs followed by a hydrophobic residue. This repetitious-sequence motif may be the basis for the observed cross-reactivity. A similar motif has been demonstrated to be the basis for antibody cross-reactivity between glutamate-rich proteins of P. falciparum. The expression of multiple glutamate-rich proteins with cross-reacting epitopes may be a general phenomenon in Plasmodium species.

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A cDNA putatively encoding the Pc90 erythrocyte membrane antigen ofPlasmodium chabaudi

Cited by 6 publications

References 8 publications

Hepatic miRNA expression reprogrammed by Plasmodium chabaudi malaria

Hepatic miRNA expression reprogrammed by Plasmodium chabaudi malaria

Ten families of variant genes encoded in subtelomeric regions of multiple chromosomes of Plasmodium chabaudi, a malaria species that undergoes antigenic variation in the laboratory mouse

Characterization of a Plasmodium chabaudi gene encoding a protein with glutamate-rich tandem repeats

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