A cell-based impedance assay for monitoring transient receptor potential (TRP) ion channel activity

Pänke, Oliver; Weigel, Winnie; Schmidt, Sabine; Steude, Anja; Robitzki, Andrea A.

doi:10.1016/j.bios.2010.10.015

Cited by 36 publications

(20 citation statements)

References 50 publications

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“…For a combined impedimetric and optical monitoring of adherent cell layers the electrode finger geometry was optimized (electrode finger width 200 mm, distance between electrode fingers 250 mm). The microelectrode arrays with transparent indium tin oxide (ITO) electrodes for the parallel impedimetric monitoring and optical live imaging were produced as previous described (Panke et al, 2011). For the present application, a layout comprising microelectrodes with 600 mm in diameter was designed and used.…”

Section: Multielectrode Array Fabricationmentioning

confidence: 99%

Quantitative impedimetric NPY-receptor activation monitoring and signal pathway profiling in living cells

Kamp

Lindner

Jahnke

et al. 2015

Biosensors and Bioelectronics

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Section: Multielectrode Array Fabricationmentioning

confidence: 99%

Quantitative impedimetric NPY-receptor activation monitoring and signal pathway profiling in living cells

Kamp

Lindner

Jahnke

et al. 2015

Biosensors and Bioelectronics

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“…Substrate-integrated electrodes can either be arranged as interdigitated comb structures (IDEs) [31,71,92,5] or use a small working electrode (WE) in combination with a large counter electrode (CE) (Fig. 2d, middle panel), as is the case for ECIS (described in more detail below) and other non-commercialized setups [119,77,80,55,38]. Both strategies are aimed at making the impedance of the electrode-solution interface dominate over contributions from the bulk resistance, thus enhancing the sensitivity of the measurements to detect subtle changes in electrode coverage with cells or morphological changes within the cell layer.…”

Section: Overview Of Endothelial Barrier Function Assaysmentioning

confidence: 99%

Impedance analysis of GPCR-mediated changes in endothelial barrier function: overview and fundamental considerations for stable and reproducible measurements

Stolwijk

Matrougui

Renken

et al. 2014

Pflugers Arch - Eur J Physiol

104

116

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The past 20 years have seen significant growth in using impedance-based assays to understand the molecular underpinning of endothelial and epithelial barrier function in response to physiological agonists, pharmacological and toxicological compounds. Most studies on barrier function use G protein coupled receptor (GPCR) agonists which couple to fast and transient changes in barrier properties. The power of impedance based techniques such as Electric Cell-Substrate Impedance Sensing (ECIS) reside in its ability to detect minute changes in cell layer integrity label-free and in real-time ranging from seconds to days. We provide a comprehensive overview of the biophysical principles, applications and recent developments in impedance-based methodologies. Despite extensive application of impedance analysis in endothelial barrier research little attention has been paid to data analysis and critical experimental variables, which are both essential for signal stability and reproducibility. We describe the rationale behind common ECIS data presentation and interpretation and illustrate practical guidelines to improve signal intensity by adapting technical parameters such as electrode layout, monitoring frequency or parameter (resistance versus impedance magnitude). Moreover, we discuss the impact of experimental parameters, including cell source, liquid handling and agonist preparation on signal intensity and kinetics. Our discussions are supported by experimental data obtained from human microvascular endothelial cells challenged with three GPCR agonists, thrombin, histamine and Sphingosine-1-Phosphate.

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“…Varshney and Li showed that the impedance change of Escherichia coli cells is due to the growth and concentration of the cells [17]. Asphahani et al and Panke et al indicated that the adhesion and spreading of cells significantly affect the impedance characteristics obtained using cell-based sensors [18,19]. Hong et al distinguished A549, MDA-MB-231, MCF-7, and HeLa cells using impedance spectra [20].…”

Section: Introductionmentioning

confidence: 96%

Analytical and Numerical Modeling Methods for Electrochemical Impedance Analysis of Single Cells on Coplanar Electrodes

et al. 2014

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Impedance measurements provide basic electrical properties and are used to analyze the characteristics of electrochemical materials for biomedical applications. The extracellular fluid (ECF) in microfluidic devices greatly affects the accuracy of impedance measurements of cells. When a single cell is placed in large amounts of ECF, the electric current mostly passes through the ECF, not the cell. Hence, this work presents a modeling method that is demonstrated in numerical and analytical solutions for eliminating the effect of ECF in coplanar impedance sensors. The proposed modeling method uses fundamental formulas of circuits that include the electrical parameters of the ECF, cytoplasm, and cell membrane. Equivalent circuit models for the coplanar impedance sensor are established to simulate the impedance as well as the measured ones for excitation frequencies in the range of 11–101 kHz. According to the calculation result using the proposed modeling method, the cytoplasm resistance, membrane capacitance, medium resistance, and medium capacitance of HeLa (human cervix adenocarcinoma) cell are 13.5 kΩ, 122.6 pF, 27.9 kΩ, and 337.7 pF, respectively. Moreover, the electric current distribution in the coplanar impedance sensor is investigated using finite element method (FEM) simulation software. The variation in the impedance during measurements with the simultaneous application of an alternating‐current (AC) voltage amplitude of 0.4 Vpp in the fluid volume range of 9–144 µL is also studied.

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A cell-based impedance assay for monitoring transient receptor potential (TRP) ion channel activity

Cited by 36 publications

References 50 publications

Quantitative impedimetric NPY-receptor activation monitoring and signal pathway profiling in living cells

Quantitative impedimetric NPY-receptor activation monitoring and signal pathway profiling in living cells

Impedance analysis of GPCR-mediated changes in endothelial barrier function: overview and fundamental considerations for stable and reproducible measurements

Analytical and Numerical Modeling Methods for Electrochemical Impedance Analysis of Single Cells on Coplanar Electrodes

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