A chalcone-syringaldehyde hybrid inhibits triple-negative breast cancer cell proliferation and migration by inhibiting CKAP2-mediated FAK and STAT3 phosphorylation

Jin, Xiangxiang; Mei, Yanan; Shen, Zhe; Zhu, Jufan; Xing, Sunhui; Yang, Huamao; Liang, Guang; Zheng, Xiaohui

doi:10.1016/j.phymed.2022.154087

Cited by 16 publications

(7 citation statements)

References 32 publications

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“…A full genome RNA-seq analysis found that 4% of the affected genes were related to DNA damage and repair and that 18% of the altered genes were functionally related to cell adhesion and migration. In tests, CSH1 administration was observed to significantly alter FAK distribution and phosphorylation, which stopped TNBC cells from migrating and decreased cellmatrix adhesion (32) . The compound under study scopoletin has function groups (hydroxyl group, methoxy group and carbonyl group aromatic ring) similar to CSH1, so we expect it to act in the same mechanism as CSH1, for that we agree with this study.…”

Section: Discussionmentioning

confidence: 96%

In Vitro Novel Study the Effect of Scopoletin Compound and Silver Nanoparticles of Fenugreek Extract on Focal Adhesion Kinase Enzyme as Anti-Metastatic in Serum of Iraqi Patients of Stage ιι and Stage ιιι with Pancreatic Cancer

Hasan¹,

AL-Saarag²

2023

The Egyptian Journal of Hospital Medicine

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Background: Experts consider pancreatic cancer one of the most deadly types. For unknown reasons, pancreatic cancer incidence and mortality are rising. Scopoletin and Ag-NPs compounds have therapeutic properties and are utilized experimentally in vitro as chemotherapies for numerous disorders.Objective: This study manufactures silver nanoparticles from fenugreek extract and analyzes their anti-metastatic effects with scopoletin at different doses to find the best combination to lower FAK enzyme concentration. Patient and Methodes: Sixty pancreatic cancer serum samples were divided: 30-Stage II patient samples (male and female, 45-65 years old), 30 stage IV patients of both sexes and ages (52-79), Thirty serum samples from healthy 23-45-years-olds served as a control group. Silver nanoparticles and scopoletin solutions were tested to find the appropriate concentration to reduce the focal adhesion kinase (FAK) enzyme. Resuts: Researchers showed that patients with stage ΙΙ and stage ΙΙΙ with pancreatic cancer and the control group had enzyme concentrations of 1.620.11ng/ml, 1.790.06 ng/ml, and 0.940.41 ng/ml, respectively, when treated with a 2 ppm solution of nanoparticles. Stage ΙΙ and stage ΙΙΙ with pancreatic cancer patients had lower enzyme concentrations than controls (1.570.15 ng/ml and 1.410.07 ng/ml). when treated with a 8 ppm solution of Scopoletin solution.Conclusion: Based on their potential to decrease focal adhesion kinase enzyme concentrations in the blood serum of patients with stageΙΙ and stageΙΙΙ Iraqi pancreatic cancer, scopoletin and silver nanoparticles of fenugreek can be recommended as anticancer materials.

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Section: Discussionmentioning

confidence: 96%

In Vitro Novel Study the Effect of Scopoletin Compound and Silver Nanoparticles of Fenugreek Extract on Focal Adhesion Kinase Enzyme as Anti-Metastatic in Serum of Iraqi Patients of Stage ιι and Stage ιιι with Pancreatic Cancer

Hasan¹,

AL-Saarag²

2023

The Egyptian Journal of Hospital Medicine

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“…CKAP2 (Cytoskeleton-associated protein 2, a crucial protein that controls cell proliferation), particularly during mitosis and cytokinesis (43). CKAP2 is up-regulated in a variety of malignant tumors and has diagnostic value in osteosarcoma (44), triplenegative breast cancer (45), gastric cancer, and other tumors (46). It has been reported that the CKAP2 gene plays a role in cell senescence (47), but its pathway needs to be confirmed by further studies.…”

Section: Discussionmentioning

confidence: 99%

ARDS and aging: TYMS emerges as a promising biomarker and therapeutic target

Li,

Yan,

Zhang

et al. 2024

Front. Immunol.

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BackgroundAcute Respiratory Distress Syndrome (ARDS) is a common condition in the intensive care unit (ICU) with a high mortality rate, yet the diagnosis rate remains low. Recent studies have increasingly highlighted the role of aging in the occurrence and progression of ARDS. This study is committed to investigating the pathogenic mechanisms of cellular and genetic changes in elderly ARDS patients, providing theoretical support for the precise treatment of ARDS.MethodsGene expression profiles for control and ARDS samples were obtained from the Gene Expression Omnibus (GEO) database, while aging-related genes (ARGs) were sourced from the Human Aging Genomic Resources (HAGR) database. Differentially expressed genes (DEGs) were subjected to functional enrichment analysis to understand their roles in ARDS and aging. The Weighted Gene Co-expression Network Analysis (WGCNA) and machine learning pinpointed key modules and marker genes, with ROC curves illustrating their significance. The expression of four ARDS-ARDEGs was validated in lung samples from aged mice with ARDS using qRT-PCR. Gene set enrichment analysis (GSEA) investigated the signaling pathways and immune cell infiltration associated with TYMS expression. Single-nucleus RNA sequencing (snRNA-Seq) explored gene-level differences among cells to investigate intercellular communication during ARDS onset and progression.ResultsARDEGs are involved in cellular responses to DNA damage stimuli, inflammatory reactions, and cellular senescence pathways. The MEmagenta module exhibited a significant correlation with elderly ARDS patients. The LASSO, RRF, and XGBoost algorithms were employed to screen for signature genes, including CKAP2, P2RY14, RBP2, and TYMS. Further validation emphasized the potential role of TYMS in the onset and progression of ARDS. Immune cell infiltration indicated differential proportion and correlations with TYMS expression. SnRNA-Seq and cell-cell communication analysis revealed that TYMS is highly expressed in endothelial cells, and the SEMA3 signaling pathway primarily mediates cell communication between endothelial cells and other cells.ConclusionEndothelial cell damage associated with aging could contribute to ARDS progression by triggering inflammation. TYMS emerges as a promising diagnostic biomarker and potential therapeutic target for ARDS.

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“…It has been reported in the literature that these five ingredients possess anti-cancer properties. Specifically, syringic acid targets glioblastoma, gastric cancer, and ovarian teratoma cancer [35][36][37]; kirenol exhibits efficacy against thyroid cancer, cancer, and myeloid leukemia [38][39][40]; chlorogenic acid shows potential against pancreatic cancer, colorectal cancer, ovarian cancer, and oral squamous carcinoma [41][42][43][44]; p-coumaric acid demonstrates activity on epidermoid carcinoma, colorectal cancer, osteosarcoma, and glioblastoma [45][46][47][48]; syringaldehyde is effective against breast cancer [49]. However, the impact of these ingredients on liver cancer needs further exploration.…”

Section: Discussionmentioning

confidence: 99%

<em>Siegesbeckia orientalis</em> Suppresses Growth and Migration through Apoptosis and Inhibition of Matrix Metalloproteinases Expression on Hepatoma in Mice

Chen,

Houng,

Chang

et al. 2024

Preprint

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This study investigated the inhibitory effect of Siegesbeckia orientalis ethanolic extract (SOE) on growth and metastasis of hepatic tumors in C57BL/6JNarl mice, which is the model of tumor-igenesis induced by subcutaneous injection of Hepa1-6 murine hepatoma cells on the back of mice. Feeding 25 mg SOE/kg BW daily for 14 days effectively reduced the hepatoma weight in tu-mor-bearing mice by 59.7%. SOE intervention reduced the expression of anti-apoptotic Bcl-2 gene in tumor tissue by 48.5% and down-regulated the antioxidant system, by reducing the gene ex-pression of catalase (48.0%), glutathione peroxidase (46.3%), and superoxide dismutase (32.0%). In addition, SOE intervention increased the production of ROS by 21.4%, and the gene expression of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 by 135.3%, 179.3% and 91.7%, respectively, indicating that SOE intervention promoted the acute inflammatory response. Results of H&E staining analysis showed that SOE had the ability to inhibit angiogenesis in tumor tissue. Fur-thermore, SOE treatment inhibited the expression of migration-related genes, such as MMP-2, MMP-7, MMP-9 and β-catenin, and the inhibition rates were 24.8%, 27.4%, 10.4% and 21.9%, respectively. These results imply that SOE had the ability to suppress tumor metastasis. On the other hand, SOE intervention decreased the serum contents of inflammatory cytokines, such as TNF-α, IL-1β and IL-6 by 27.4%, 30.4%, and 30.7%, respectively, indicating that SOE intervention has an anti-inflammatory effect on normal tissues of mice. Since SOE intervention had no signif-icant effect on liver and kidney weights, and no mice died throughout the experiment, it shows that it is safe to feed SOE under this experimental condition. Based on the above results, SOE has a great potential to be used in the treatment of hepatoma.

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A chalcone-syringaldehyde hybrid inhibits triple-negative breast cancer cell proliferation and migration by inhibiting CKAP2-mediated FAK and STAT3 phosphorylation

Cited by 16 publications

References 32 publications

In Vitro Novel Study the Effect of Scopoletin Compound and Silver Nanoparticles of Fenugreek Extract on Focal Adhesion Kinase Enzyme as Anti-Metastatic in Serum of Iraqi Patients of Stage ιι and Stage ιιι with Pancreatic Cancer

In Vitro Novel Study the Effect of Scopoletin Compound and Silver Nanoparticles of Fenugreek Extract on Focal Adhesion Kinase Enzyme as Anti-Metastatic in Serum of Iraqi Patients of Stage ιι and Stage ιιι with Pancreatic Cancer

ARDS and aging: TYMS emerges as a promising biomarker and therapeutic target

<em>Siegesbeckia orientalis</em> Suppresses Growth and Migration through Apoptosis and Inhibition of Matrix Metalloproteinases Expression on Hepatoma in Mice

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