A Challenge for Clinical Laboratories: Detection of Antifungal Resistance in<i>Candida</i>Species Causing Vulvovaginal Candidiasis

Dota, Kelen Fátima Dalben; Freitas, Alessandra R.; Consolaro, Márcia Edilaine Lopes; Svidzinski, Terezinha Inez Estivalet

doi:10.1309/lmdfca8yez0mqula

Cited by 26 publications

(23 citation statements)

References 30 publications

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“…isolated in Brazil, a correlation approaching 70% between the methods was found. In our study, the agreement was higher, exceeding 90% 6,7 . Based on these results and on studies that evaluated a high number of strains of Candida sp., we can conclude that the disk diffusion test correlated well with the other methods of assessing susceptibility.…”

contrasting

confidence: 62%

Correlation between microdilution, Etest, and disk diffusion methods for antifungal susceptibility testing of fluconazole against Candida sp. blood isolates

Menezes

Júnior

Ângelo

et al. 2013

Rev. Soc. Bras. Med. Trop.

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Introduction: Antifungal susceptibility testing assists in finding the appropriate treatment for fungal infections, which are increasingly common. However, such testing is not very widespread. There are several existing methods, and the correlation between such methods was evaluated in this study. Methods: The susceptibility to fluconazole of 35 strains of Candida sp. isolated from blood cultures was evaluated by the following methods: microdilution, Etest, and disk diffusion. Results: The correlation between the methods was around 90%. Conclusions: The disk diffusion test exhibited a good correlation and can be used in laboratory routines to detect strains of Candida sp. that are resistant to fluconazole.

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contrasting

confidence: 62%

Correlation between microdilution, Etest, and disk diffusion methods for antifungal susceptibility testing of fluconazole against Candida sp. blood isolates

Menezes

Júnior

Ângelo

et al. 2013

Rev. Soc. Bras. Med. Trop.

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“…Miconazole nitrate, an imidazole antifungal agent, has been widely used as a drug for treatment of fungal diseases. Currently, there are several reports regarding the efficacy of topical miconazole nitrate to latent and colonizing fungi, but there are no reports of soap containing the same ingredient . The miconazole soap used in this study contains 0.75% miconazole nitrate and is commercially available in Japan (Mochida Healthcare).…”

Section: Discussionmentioning

confidence: 99%

“…Currently, there are several reports regarding the efficacy of topical miconazole nitrate to latent and colonizing fungi, but there are no reports of soap containing the same ingredient. [14][15][16][17] The miconazole soap used in this study contains 0.75% miconazole nitrate and is commercially available in Japan (Mochida Healthcare). The minimum inhibitory concentration of miconazole for Candida spp.…”

Section: Discussionmentioning

confidence: 99%

Preventive effects of topical washing with miconazole nitrate‐containing soap to diaper candidiasis in hospitalized elderly patients: A prospective, double‐blind, placebo‐controlled study

Takahashi

Oyama

Tanaka³

et al. 2017

The Journal of Dermatology

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The objective of the present randomized, double-blind trial was to evaluate the efficacy and safety of daily washing with miconazole nitrate-containing soap for candidiasis at diaper-covered sites in elderly subjects under long-term inpatient care. To confirm the onset and disappearance of candidiasis, we microscopically evaluated the existence of the pseudohyphae and/or blastoconidia of Candida spp. We enrolled 75 elderly patients who wore diapers all day in the hospital or nursing home. Patients were randomly assigned to receive treatment with either miconazole soap or miconazole-free placebo soap, and 28 patients in the miconazole group and 27 patients in the placebo group were followed for 4 weeks. Although washing with miconazole soap did not affect the frequency of pseudohyphae/blastoconidia-positive patients, it significantly inhibited the positive conversion of pseudohyphae/blastoconidia compared with the placebo group. As a result, the frequency of patients positive for pseudohyphae/blastoconidia was significantly lower in the miconazole group than in the control group at 4 weeks (17.9% vs 44.4%). Clinically apparent diaper candidiasis did not develop in either group. Washing with miconazole soap was a significant independent factor for reducing the cases positive for pseudohyphae/blastoconidia, while diarrhea and heart failure were significant factors associated with an increase in the positive rate at the end-point. Severe adverse effects were not found in any patients. Thus, washing with miconazole soap is well-tolerated and can inhibit the positive conversion of Candida in patients wearing diapers. Therefore, maintenance of genital hygiene using this soap may prophylactically decrease the overall prevalence of patients with diaper candidiasis.

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“…isoladas no Brasil, foi encontrada uma concordância que se aproxima de 70% entre os métodos (DOTA et al, 2011;NEGRI et al, 2009), valor muito inferior ao que obtivemos. Todos esses estudos referemse ao fluconazol, pois a anfotericina B ainda se encontra em processo de padronização.…”

Section: Discussionunclassified

Comparação entre microdiluição e disco difusão para o teste de susceptibilidade aos antifúngicos contra Candida spp.

Júnior

Menezes

Cunha

et al. 2012

Semin. Cienc. Biol. Saude

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A evolução da resistência fúngica pode ser avaliada pelo teste de susceptibilidade aos antifúngicos. Os protocolos mais utilizados para testes de susceptibilidade são a micro diluição em caldo (MDC), disco difusão (DD) e E-test. O objetivo principal deste estudo foi comparar os testes de sensibilidade realizados pelos métodos de MDC e DD para a anfotericina B e o fluconazol contra cepas de Candida spp. isoladas em Fortaleza/CE. Foram utilizadas 40 C. albicans, 50 C. tropicalis e 47 C. parapsilosis, isoladas de amostras de sangue e urina de pacientes atendidos no Hospital Geral de Fortaleza. Os testes foram realizados pelos métodos MDC e DD, de acordo com os protocolos M27-A3 e M44-A2 do CLSI. As metodologias foram comparadas e calculadas os percentuais de concordância. Para a anfotericina B e fluconazol, ocorreu um predomínio de cepas com susceptibilidade. O percentual de concordância para as metodologias foi acima de 97% e não foram detectados erros graves. A utilização de DD para realização de testes de susceptibilidade antifúngica pode ser aplicada na rotina laboratorial, pois é uma técnica fácil, barata, e de confiança quando comparada ao teste de MDC, sem comprometer os resultados para as cepas de C. albicans, C. tropicalis e C. parapsilosis. Palavras-chave: Microdiluição. Disco difusão. Candida sp.

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A Challenge for Clinical Laboratories: Detection of Antifungal Resistance inCandidaSpecies Causing Vulvovaginal Candidiasis

Cited by 26 publications

References 30 publications

Correlation between microdilution, Etest, and disk diffusion methods for antifungal susceptibility testing of fluconazole against Candida sp. blood isolates

Correlation between microdilution, Etest, and disk diffusion methods for antifungal susceptibility testing of fluconazole against Candida sp. blood isolates

Preventive effects of topical washing with miconazole nitrate‐containing soap to diaper candidiasis in hospitalized elderly patients: A prospective, double‐blind, placebo‐controlled study

Comparação entre microdiluição e disco difusão para o teste de susceptibilidade aos antifúngicos contra Candida spp.

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