A charged-particle microbeam: II. A single-particle micro-collimation and detection system.

Folkard, M.; Vojnovic, B.; Hollis, Kendall J.; Bowey, A.G.; Watts, S.; Schettino, Giuseppe; Prise, Kevin M.; Michael, B.D.

doi:10.1080/095530097143167

Cited by 110 publications

(10 citation statements)

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“…The Gray Cancer Institute charged particle microbeam system was used for targeted irradiation [ 27 , 28 ]. Cells were seeded in the centre of Mylar foil dishes pretreated with 1.7 µg/cm 2 BD Cell-Tak adhesive (Becton Dickinson, Erembodegem, Belgium) and stained in medium containing a 0.8 µM concentration of the fluorescent dye Hoechst 33342 (Molecular Probes, Leiden, the Netherlands) to enable visualisation of cell nuclei by fluorescent microscopy at the microbeam stage.…”

Section: Methodsmentioning

confidence: 99%

Radioprotection of targeted and bystander cells by methylproamine

et al. 2014

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IntroductionRadioprotective agents are of interest for application in radiotherapy for cancer and in public health medicine in the context of accidental radiation exposure. Methylproamine is the lead compound of a class of radioprotectors which act as DNA binding anti-oxidants, enabling the repair of transient radiation-induced oxidative DNA lesions. This study tested methylproamine for the radioprotection of both directly targeted and bystander cells.MethodsT98G glioma cells were treated with 15 μM methylproamine and exposed to 137Cs γ-ray/X-ray irradiation and He2+ microbeam irradiation. Radioprotection of directly targeted cells and bystander cells was measured by clonogenic survival or γH2AX assay.ResultsRadioprotection of directly targeted T98G cells by methylproamine was observed for 137Cs γ-rays and X-rays but not for He2+ charged particle irradiation. The effect of methylproamine on the bystander cell population was tested for both X-ray irradiation and He2+ ion microbeam irradiation. The X-ray bystander experiments were carried out by medium transfer from irradiated to non-irradiated cultures and three experimental designs were tested. Radioprotection was only observed when recipient cells were pretreated with the drug prior to exposure to the conditioned medium. In microbeam bystander experiments targeted and nontargeted cells were co-cultured with continuous methylproamine treatment during irradiation and postradiation incubation; radioprotection of bystander cells was observed.Discussion and conclusionMethylproamine protected targeted cells from DNA damage caused by γ-ray or X-ray radiation but not He2+ ion radiation. Protection of bystander cells was independent of the type of radiation which the donor population received.

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Section: Methodsmentioning

confidence: 99%

Radioprotection of targeted and bystander cells by methylproamine

et al. 2014

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“…The Gray Cancer Institute charged-particle microbeam facility was used for this study. Details of the experimental setup for nuclear targeting using the microbeam have been described previously (31, 32). Nonconfluent cells were seeded in a 6 × 6-mm area of a specially designed Mylar-based microbeam dish preincubated overnight with cell culture media.…”

Section: Methodsmentioning

confidence: 99%

Cytoplasmic Irradiation Induces Mitochondrial-Dependent 53BP1 Protein Relocalization in Irradiated and Bystander Cells

et al. 2007

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The accepted paradigm for radiation effects is that direct DNA damage via energy deposition is required to trigger the downstream biological consequences. The radiation-induced bystander effect is the ability of directly irradiated cells to interact with their nonirradiated neighbors, which can then show responses similar to those of the targeted cells. p53 binding protein 1 (53BP1) forms foci at DNA double-strand break sites and is an important sensor of DNA damage. This study used an ionizing radiation microbeam approach that allowed us to irradiate specifically the nucleus or cytoplasm of a cell and quantify response in irradiated and bystander cells by studying ionizing radiation-induced foci (IRIF) formation of 53BP1 protein. Our results show that targeting only the cytoplasm of a cell is capable of eliciting 53BP1 foci in both hit and bystander cells, independently of the dose or the number of cells targeted. Therefore, direct DNA damage is not required to trigger 53BP1 IRIF. The use of common reactive oxygen species and reactive nitrogen species (RNS) inhibitors prevent the formation of 53BP1 foci in hit and bystander cells.

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“…Roeske and Stinchcomb (1996) have demonstrated that z 0 can be calculated from D 0 . Recent experimental advances allow for z 0 to be measured directly (Folkard et al 1997, Hei et al 1997, Pugliese et al 1997, Soyland et al 2000, Prise et al 2002, Wang et al 2003. These experiments use either track-etch techniques with computerized processing (Soyland et al 2000, Wang et al 2003 or microbeams (Folkard et al 1997, Hei et al 1997, Pugliese et al 1997, Prise et al 2002 to determine z 0 .…”

Section: Discussionmentioning

confidence: 99%

Laser Polarization Effect on Adiabatic Inversion of Degenerate Two-Level System

Niki¹,

Kitazima²

1996

Jpn. J. Appl. Phys.

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Alpha-particle emitters are currently being considered for the treatment of micrometastatic disease. Based on in vitro studies, it has been speculated that only a few alpha-particle hits to the cell nucleus are considered lethal. However, such estimates do not consider the stochastic variations in the number of alpha-particle hits, energy deposited, or in the cell survival process itself. Using a tumour control probability (TCP) model for alpha-particle emitters, we derive an estimate of the average number of hits to the cell nucleus required to provide a high probability of eradicating a tumour cell population. In simulation studies, our results demonstrate that the average number of hits required to achieve a 90% TCP for 10 4 clonogenic cells ranges from 18 to 108. Those cells that have large cell nuclei, high radiosensitivities and alpha-particle emissions occurring primarily in the nuclei tended to require more hits. As the clinical implementation of alpha-particle emitters is considered, this type of analysis may be useful in interpreting clinical results and in designing treatment strategies to achieve a favourable therapeutic outcome.

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A charged-particle microbeam: II. A single-particle micro-collimation and detection system.

Cited by 110 publications

References 1 publication

Radioprotection of targeted and bystander cells by methylproamine

Radioprotection of targeted and bystander cells by methylproamine

Cytoplasmic Irradiation Induces Mitochondrial-Dependent 53BP1 Protein Relocalization in Irradiated and Bystander Cells

Laser Polarization Effect on Adiabatic Inversion of Degenerate Two-Level System

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