A chemical-detecting system based on a cross-reactive optical sensor array

Dickinson, Todd A.; White, Joel; Kauer, John S.; Walt, David R.

doi:10.1038/382697a0

Cited by 385 publications

(254 citation statements)

References 28 publications

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“…Testing the validity of FreeSRF. As with other models (37,(69)(70)(71)(72), it was necessary to use a learning set to establish the appropriate relationships and weighting parameters for FreeSRF. In our case, we determined χ from ρ exp for a training set of well-characterized binding systems (Table 1).…”

Section: Conformation and Hydration Changes Are The Origin Of Free-somentioning

confidence: 99%

Origin and prediction of free-solution interaction studies performed label-free

Bornhop

Kammer

Kussrow

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Interaction/reaction assays have led to significant scientific discoveries in the biochemical, medical, and chemical disciplines. Several fundamental driving forces form the basis of intermolecular and intramolecular interactions in chemical and biochemical systems (London dispersion, hydrogen bonding, hydrophobic, and electrostatic), and in the past three decades the sophistication and power of techniques to interrogate these processes has developed at an unprecedented rate. In particular, label-free methods have flourished, such as NMR, mass spectrometry (MS), surface plasmon resonance (SPR), biolayer interferometry (BLI), and backscattering interferometry (BSI), which can facilitate assays without altering the participating components. The shortcoming of most refractive index (RI)-based label-free methods such as BLI and SPR is the requirement to tether one of the interaction entities to a sensor surface. This is not the case for BSI. Here, our hypothesis is that the signal origin for freesolution, label-free determinations can be attributed to conformation and hydration-induced changes in the solution RI. We propose a model for the free-solution response function (FreeSRF) and show that, when quality bound and unbound structural data are available, FreeSRF correlates well with the experiment (R 2 > 0.99, Spearman rank correlation coefficients >0.9) and the model is predictive within ∼15% of the experimental binding signal. It is also demonstrated that a simple mass-weighted dη/dC response function is the incorrect equation to determine that the change in RI is produced by binding or folding event in free solution.backscattering interferometry | assay methodology | molecular interactions | conformation change | hydration change C ontemporary assays enabling single-molecule detection (1,2) have accelerated the sequencing of the human genome (3) and facilitated imaging with extraordinary resolution without labels (4). To most closely approximate the natural state, an interaction assay methodology would interrogate the processes (reaction, molecular interaction, protein folding event, etc.) without perturbation. Label-free chemical and biochemical investigations (5, 6) transduce the desired signal without an exogenous label (fluorescent, radioactive, or otherwise) representing an essential step toward this goal. Many label-free methods require one of the interacting species to be either tethered or immobilized to the sensor surface, introducing a potential perturbation to the natural state of the species (7,8). However, back-scattering interferometry (BSI) is a free-solution label-free technique with the added benefit of sensitivity that rivals fluorescence (9). There are other techniques performed in free solution, such as MS (10, 11) and NMR (12,13) and the widely used isothermal titration calorimetry (ITC) (14, 15). As with NMR, ITC has many advantages, but exhibits modest sensitivity and often requires large sample quantities. Another increasingly popular free-solution approach is microscale thermophoresis (...

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Section: Conformation and Hydration Changes Are The Origin Of Free-somentioning

confidence: 99%

Origin and prediction of free-solution interaction studies performed label-free

Bornhop

Kammer

Kussrow

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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“…Detector types of interest include carbon black-insulating polymer composites [1], conducting organic polymers [2][3][4], polymer-coated quartz crystal microbalances (QCM) [5], polymer-coated surface acoustic wave (SAW) devices [6,7], polymer-coated capacitors [8], and arrays of dye-impregnated polymeric beads or coated optical fibers [9][10][11]. The responses of such sorption-based detectors depend primarily on the partition coefficient of the gaseous analyte into the polymer [12].…”

Section: Introductionmentioning

confidence: 99%

Exploitation of spatiotemporal information and geometric optimization of signal/noise performance using arrays of carbon black-polymer composite vapor detectors

Briglin

Freund

Tokumaru

et al. 2002

Sensors and Actuators B: Chemical

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We have investigated various aspects of the geometric and spatiotemporal response properties of an array of sorption-based vapor detectors. The detectors of specific interest are composites of insulating organic polymers filled with electrical conductors, wherein the detector film provides a reversible dc electrical resistance change upon the sorption of an analyte vapor. An analytical expression derived for the signal/noise performance as a function of detector volume implies that there is an optimum detector film volume which will produce the highest signal/noise ratio for a given carbon black-polymer composite when exposed to a fixed volume of sampled analyte. This prediction has been verified experimentally by exploring the response behavior of detectors having a variety of different geometric form factors. We also demonstrate that useful information can be obtained from the spatiotemporal response profile of an analyte moving at a controlled flow velocity across an array of chemically identical, but spatially nonequivalent, detectors. Finally, we demonstrate the use of these design principles, incorporated with an analysis of the changes in detector signals in response to variations in analyte flow rate, to obtain useful information on the composition of analytes and analyte mixtures. #

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“…Some of the Schiff-base complexes possess interesting nonlinear optical [5,6] and magnetic properties [7,8]. On the other hand, fluorescent complexes have been extensively studied mainly because they may be good candidates for fluorescent sensors and electroluminescent (EL) devices [9,10]. Zinc Schiff-base complexes exhibited strong electroluminescence as well as photoluminescence [1113] which are strongly coupled to molecular structures and bulk molecular packing characteristics [14,15].…”

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confidence: 99%

Synthesis, structure, optoelectronic properties of novel zinc Schiff-base complexes

et al. 2013

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Two novel zinc Schiff-base complexes, bis-(N-(2-hydroxybenzidene)-p-aminodimethylaniline)zinc(II) (2) and bis-(N-(2-hydroxy-1-naphthidene)-p-aminodimethylaniline)zinc(II) (4) were designed and synthesized. Both the complexes exhibit good solubility in organic solvents and excellent thermal stabilities. A single crystal of 2 was grown and its crystalline structure was determined from X-ray diffraction data. Analysis of the electronic structures of both the zinc complexes calculated by density functional theory reveals a localization of orbital. The UV-Vis absorption and photoluminescence profiles of 4 in thin film are similar to those of 2, but the emission for 4 is red-shifted compared to 2. Three-layered devices with a configuration of ITO/NPB/2/Alq 3 /LiF/Al and ITO/NPB/4/Alq 3 /LiF/Al show a yellow and red emission, respectively. zinc complexes, Schiff-base ligands, electroluminescence Citation:Wang L P, Jiao S B, Zhang W F, et al. Synthesis, structure, optoelectronic properties of novel zinc Schiff-base complexes.

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A chemical-detecting system based on a cross-reactive optical sensor array

Cited by 385 publications

References 28 publications

Origin and prediction of free-solution interaction studies performed label-free

Origin and prediction of free-solution interaction studies performed label-free

Exploitation of spatiotemporal information and geometric optimization of signal/noise performance using arrays of carbon black-polymer composite vapor detectors

Synthesis, structure, optoelectronic properties of novel zinc Schiff-base complexes

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