A Chip Digital PCR Assay for Quantification of Common Wheat Contamination in Pasta Production Chain

Morcia, Caterina; Bergami, Raffaella; Scaramagli, Sonia; Ghizzoni, Roberta; Carnevali, Paola; Terzi, Valeria

doi:10.3390/foods9070911

Cited by 17 publications

(20 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pierboni et al [ 93 ] efficiently applied droplet digital PCR to detect the presence of peanut and soybean allergens in mill and bakery products and demonstrated the usefulness of this technique for the food safety of allergic populations. More recently, Morcia et al [ 47 ] developed a duplex chip digital PCR assay able to identify and quantify common wheat presence along the whole pasta production chain. The authors found that the limit of detection of the proposed method was 0.3% common wheat contamination, whereas the limit of quantification was found at the 1.5% level.…”

Section: Molecular Approaches To Agri-food Analysismentioning

confidence: 99%

Molecular Approaches to Agri-Food Traceability and Authentication: An Updated Review

Fanelli

Mascio

Miazzi

et al. 2021

Foods

View full text Add to dashboard Cite

In the last decades, the demand for molecular tools for authenticating and tracing agri-food products has significantly increased. Food safety and quality have gained an increased interest for consumers, producers, and retailers, therefore, the availability of analytical methods for the determination of food authenticity and the detection of major adulterations takes on a fundamental role. Among the different molecular approaches, some techniques such as the molecular markers-based methods are well established, while some innovative approaches such as isothermal amplification-based methods and DNA metabarcoding have only recently found application in the agri-food sector. In this review, we provide an overview of the most widely used molecular techniques for fresh and processed agri-food authentication and traceability, showing their recent advances and applications and discussing their main advantages and limitations. The application of these techniques to agri-food traceability and authentication can contribute a great deal to the reassurance of consumers in terms of transparency and food safety and may allow producers and retailers to adequately promote their products.

show abstract

Section: Molecular Approaches To Agri-food Analysismentioning

confidence: 99%

Molecular Approaches to Agri-Food Traceability and Authentication: An Updated Review

Fanelli

Mascio

Miazzi

et al. 2021

Foods

View full text Add to dashboard Cite

show abstract

“…Two genic targets for durum wheat and oat were selected from the literature or newly developed. The Grano CO2 assay, designed on TaHd1 gene sequence and developed by Morcia et al [ 18 ] was used to track Triticum genus. A new assay (Avena dig assay) was designed on actin1 gene sequence to track Avena genus.…”

Section: Resultsmentioning

confidence: 99%

Moving from qPCR to Chip Digital PCR Assays for Tracking of some Fusarium Species Causing Fusarium Head Blight in Cereals

Morcia¹,

Tumino²,

Gasparo³

et al. 2020

Microorganisms

Self Cite

View full text Add to dashboard Cite

Fusarium Head Blight (FHB) is one of the major diseases affecting small-grain cereals, worldwide spread and responsible for severe yield and quality losses annually. Diagnostic tools, able to track Fusarium species even in the early stages of infection, can contribute to mycotoxins’ risk control. Among DNA-based technologies for Fusarium detection, qPCR (single and multiplex assays) is currently the most applied method. However, pathogen diagnostics is now enforced by digital PCR (dPCR), a breakthrough technology that provides ultrasensitive and absolute nucleic acid quantification. In our work, a panel of chip digital PCR assays was developed to quantify Fusarium graminearum, F.culmorum, F. sporotrichioides, F. poae and F. avenaceum. The primers/probes combinations were evaluated on pure fungal samples with cdPCR technique, in comparison with the qPCR approach. Moreover, the cdPCR assays were applied to quantify Fusarium in durum wheat and oat samples, naturally contaminated or spiked with fungal DNA. For a better evaluation of infection level in plants, duplex assays were developed, able to co-amplify both plant and fungal DNA. To the best of our knowledge, this is the first study directed to the application of digital PCR to Fusarium diagnosis in plants.

show abstract

“…A pillar of Italian durum wheat pasta production chain is, however, the grain identity, which is mandatory at the species level. The use of durum wheat semolina is, in fact, compulsory for Italian pasta, in which the Triticum aestivum species is considered a contamination that must not exceed the 3% maximum level [ 4 ]. However, the quality of the end products can be related not only to species, but also to the cultivar [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

A Fast, Naked-Eye Assay for Varietal Traceability in the Durum Wheat Production Chain

Cibecchini¹,

Cecere²,

Tumino³

et al. 2020

Foods

Self Cite

View full text Add to dashboard Cite

The development of a colorimetric mono-varietal discriminating assay, aimed at improving traceability and quality control checks of durum wheat products, is described. A single nucleotide polymorphism (SNP) was identified as a reliable marker for wheat varietal discrimination, and a rapid test for easy and clear identification of specific wheat varieties was developed. Notably, an approach based on the loop-mediated isothermal amplification reaction (LAMP) as an SNP discrimination tool, in combination with naked-eye visualization of the results, was designed and optimized. Our assay was proven to be effective in the detection of adulterated food products, including both substitution and mixing with different crop varieties.

show abstract

A Chip Digital PCR Assay for Quantification of Common Wheat Contamination in Pasta Production Chain

Cited by 17 publications

References 25 publications

Molecular Approaches to Agri-Food Traceability and Authentication: An Updated Review

Molecular Approaches to Agri-Food Traceability and Authentication: An Updated Review

Moving from qPCR to Chip Digital PCR Assays for Tracking of some Fusarium Species Causing Fusarium Head Blight in Cereals

A Fast, Naked-Eye Assay for Varietal Traceability in the Durum Wheat Production Chain

Contact Info

Product

Resources

About