A chitosan-based multifunctional hydrogel containing <i>in situ</i> rapidly bioreduced silver nanoparticles for accelerating infected wound healing

Zhao, Fengxin; Liu, Yifan; Song, Tao; Zhang, Bin; Li, Dongxiao; Xiao, Yumei; Zhang, Xingdong

doi:10.1039/d1tb02850b

Cited by 39 publications

(24 citation statements)

References 52 publications

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“…Zhao et al 2022 recently published a chitosan-based in situ hydrogel containing silver nanoparticles for wound healing . In this study, they used harsh chemicals such as acetic acid . Somayajula et al published a study in 2019, in which they employed sodium borohydrate to reduce silver ions prior to hydrogel synthesis .…”

Section: Resultsmentioning

confidence: 99%

“…Hydrogels are hydrophilic polymeric materials that can hold a large volume of water in their three-dimensional networks . Lately, research has been carried out for various biomedical applications of hydrogels including tissue engineering as well as drug delivery. − Importantly, the hydrogels used as drug delivery systems have shown diverse advantages in antimicrobial photodynamic therapy . Hydrogel-based drug delivery systems are highly advantageous for PDT in comparison to other conventional drug carriers.…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…3 In this study, they used harsh chemicals such as acetic acid. 67 Somayajula et al published a study in 2019, in which they employed sodium borohydrate to reduce silver ions prior to hydrogel synthesis. 68 The main advantage of our in situ strategy is avoidance of the use of harsh chemicals and multiple steps.…”

Section: In Situ Synthesis Of Silver Nanoparticle Embedded Lignin Hyd...mentioning

confidence: 99%

“…For the validation of the mechanism of antimicrobial photodynamic activity, the ROS generation ability of the nanocomposite hydrogels as well as bare lignin hydrogels was elucidated through the 2,7-dichlorofluorescin diacetate (DCFDA) assay. 67 Briefly, the E. coli cell suspensions were collected at the log phase stage (0.6, OD 600 ). This was followed by the addition of DCFDA (final concentration of 20 μM) to the E. coli cell suspension and incubation in an orbital shaker at 37 °C for 20 min.…”

Section: Elucidation Of the Mechanism Of Antimicrobial Photodynamic A...mentioning

confidence: 99%

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Photodynamic Lignin Hydrogels: A Versatile Self-Healing Platform for Sustained Release of Photosensitizer Nanoconjugates

Chandna

Paul

Kaur

et al. 2022

ACS Appl. Polym. Mater.

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A multifunctional hydrogel that combines the properties of pH-responsiveness as well as controlled release holds enormous potential for antimicrobial photodynamic therapy through the development of wound dressings and coatings. Utilization of lignin (a biodegradable and cost-effective biopolymer) could be advantageous as a sustainable alternative over the conventional hydrogels for photodynamic therapy, which is an underexplored area. In this work, the lignin-based hydrogel was developed, which was found to have remarkable self-healing properties. Moreover, the lignin-based photodynamic hydrogels designed via in situ and ex situ methods were found to be transparent enough (through the variation of lignin concentration) to be utilized for photodynamic therapy applications. The in situ method is an example of lignin hydrogel synthesis which is advantageous as it saves multiple reaction steps of nanoparticle synthesis and incorporation into the hydrogel. However, for the demonstration of photodynamic effect, the lignin-based hydrogels were doped with a photosensitizer (Rose Bengal, RB) and also with RB-conjugated lignin-derived silver nanocomplexes (RB@L-AgNCs). The developed lignin-based nanocomposite hydrogels were characterized through various methods including UV–vis spectroscopy, HRTEM, BET, XRD, FESEM, rheology, and FTIR to determine their material properties. Interestingly, the lignin hydrogels were found to possess pH-responsive properties for controlled release of the incorporated nanoagents. Antimicrobial photodynamic therapy studies were performed using lignin-based nanocomposite hydrogels as well as bare lignin hydrogels through utilization of a green laser for a short time (3 min). Mechanistically, it was observed that the nanocomposite-doped hydrogel worked much better as compared to the native lignin hydrogel due to higher reactive oxygen species (ROS) generation. Furthermore, the antimicrobial photodynamic activity of the developed lignin-based nanocomposite hydrogels was validated through fluorescence microscopy studies (live–dead cell imaging). These developed biodegradable lignin-based nanocomposite hydrogels can be efficiently used for the development of wound dressings and nanocoatings over various surfaces for stimuli responsive antimicrobial effect.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: In Situ Synthesis Of Silver Nanoparticle Embedded Lignin Hyd...mentioning

confidence: 99%

Section: Elucidation Of the Mechanism Of Antimicrobial Photodynamic A...mentioning

confidence: 99%

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Photodynamic Lignin Hydrogels: A Versatile Self-Healing Platform for Sustained Release of Photosensitizer Nanoconjugates

Chandna

Paul

Kaur

et al. 2022

ACS Appl. Polym. Mater.

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Advances in Gelatin Bioinks to Optimize Bioprinted Cell Functions

Asim

Tabish

Liaqat

et al. 2023

Adv Healthcare Materials

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Gelatin is a widely utilized bioprinting biomaterial due to its cell‐adhesive and enzymatically cleavable properties, which improve cell adhesion and growth. Gelatin is often covalently cross‐linked to stabilize bioprinted structures, yet the covalently cross‐linked matrix is unable to recapitulate the dynamic microenvironment of the natural extracellular matrix (ECM), thereby limiting the functions of bioprinted cells. To some extent, a double network bioink can provide a more ECM‐mimetic, bioprinted niche for cell growth. More recently, gelatin matrices are being designed using reversible cross‐linking methods that can emulate the dynamic mechanical properties of the ECM. This review analyzes the progress in developing gelatin bioink formulations for 3D cell culture, and critically analyzes the bioprinting and cross‐linking techniques, with a focus on strategies to optimize the functions of bioprinted cells. This review discusses new cross‐linking chemistries that recapitulate the viscoelastic, stress‐relaxing microenvironment of the ECM, and enable advanced cell functions, yet are less explored in engineering the gelatin bioink. Finally, this work presents the perspective on the areas of future research and argues that the next generation of gelatin bioinks should be designed by considering cell–matrix interactions, and bioprinted constructs should be validated against currently established 3D cell culture standards to achieve improved therapeutic outcomes.

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Flexible Organic Photovoltaic‐Powered Hydrogel Bioelectronic Dressing With Biomimetic Electrical Stimulation for Healing Infected Diabetic Wounds

Hu,

Wang,

Yang

et al. 2023

Advanced Science

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Electrical stimulation (ES) is proposed as a therapeutic solution for managing chronic wounds. However, its widespread clinical adoption is limited by the requirement of additional extracorporeal devices to power ES‐based wound dressings. In this study, a novel sandwich‐structured photovoltaic microcurrent hydrogel dressing (PMH dressing) is designed for treating diabetic wounds. This innovative dressing comprises flexible organic photovoltaic (OPV) cells, a flexible micro–electro–mechanical systems (MEMS) electrode, and a multifunctional hydrogel serving as an electrode–tissue interface. The PMH dressing is engineered to administer ES, mimicking the physiological injury current occurring naturally in wounds when exposed to light; thus, facilitating wound healing. In vitro experiments are performed to validate the PMH dressing's exceptional biocompatibility and robust antibacterial properties. In vivo experiments and proteomic analysis reveal that the proposed PMH dressing significantly accelerates the healing of infected diabetic wounds by enhancing extracellular matrix regeneration, eliminating bacteria, regulating inflammatory responses, and modulating vascular functions. Therefore, the PMH dressing is a potent, versatile, and effective solution for diabetic wound care, paving the way for advancements in wireless ES wound dressings.

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A chitosan-based multifunctional hydrogel containing in situ rapidly bioreduced silver nanoparticles for accelerating infected wound healing

Abstract: Generally, bacterial infection seriously hinders the wound healing process, so it is crucial to safeguard the wound from severe infection. Besides, multifunctional hydrogel dressings (self-healing, injectable, antibacterial and adaptable) seem...

Cited by 39 publications

References 52 publications

Photodynamic Lignin Hydrogels: A Versatile Self-Healing Platform for Sustained Release of Photosensitizer Nanoconjugates

Photodynamic Lignin Hydrogels: A Versatile Self-Healing Platform for Sustained Release of Photosensitizer Nanoconjugates

Advances in Gelatin Bioinks to Optimize Bioprinted Cell Functions

Flexible Organic Photovoltaic‐Powered Hydrogel Bioelectronic Dressing With Biomimetic Electrical Stimulation for Healing Infected Diabetic Wounds

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