A Chitosan-Hyaluronan-Based Hydrogel-Hydrocolloid SupportsIn VitroCulture and Differentiation of Human Mesenchymal Stem/Stromal Cells

Abstract

Three-dimensional (3D) cell culture platforms are increasingly utilized due to their ability to more closely mimic the in vivo microenvironment compared to traditional two-dimensional methods. Limitations of currently available 3D materials include lack of cell attachment, long polymerization times, and inclusion of undefined xenobiotics, and cytotoxic cross-linkers. Evaluated here is a unique hydrogel comprised of polyelectrolytic complex (PEC) fibers formed by hyaluronic acid and chitosan (CT). When hydrated with fetal bovine serum containing human mesenchymal stem/stromal cells (hMSCs), a hydrogel with an elastic modulus of 264±38 Pa formed in seconds with cells distributed throughout the matrix. Scanning electron microscopy showed a lattice-like meshwork of PEC fibers forming irregular compartments. hMSCs showed 48% viability during the first 24 h, with cell populations thereafter reaching a steady state for 14 days. hMSCs in the matrix were induced to differentiate to chondrogenic, osteogenic, and adipogenic phenotypes. Emergent features, at days 56 and 70, consisted of chondrogenesis on the surface of hydrogels induced to osteogenic and adipogenic phenotypes. Results indicate that this matrix may be useful for tissue engineering and disease modeling applications.

Osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells encapsulated in Thai silk fibroin/collagen hydrogel: a pilot study in vitro

“…Cell numbers then plateaued and were maintained until day 14 of culture. This indicated a balance between apoptotic cell death and proliferation similar as found in culture of human MSCs in a chitosanhyaluronan-based hydrogel in vitro [22]. The cell density at plateau phase was approximately 20%-30% of loaded cells.…”

In vitro interaction of human Wharton's jelly mesenchymal stem cells with biomimetic 3D scaffold

“…Some researchers have reported that increasing the amount of gelatin in the scaffold can induce the binding and adhesion of the cells to the surface . Chitosan is a cationic polysaccharide capable of interacting with the surface anions and connecting the cell to the surface . Several studies have shown that Cs effective being in cell adhesion, its effect is, however, weaker than gel .…”

“…45 Chitosan is a cationic polysaccharide capable of interacting with the surface anions and connecting the cell to the surface. 46 Several studies have shown that Cs effective being in cell adhesion, its effect is, however, weaker than gel. 6,8 Nano-Hydroxyapatite is a bioceramic compound that comprises the natural mineral component of the bone.…”

Rapid Induction of Cerebral Organoids From Human Induced Pluripotent Stem Cells Using a Chemically Defined Hydrogel and Defined Cell Culture Medium

“…Cell‐Mate3D hydrogels embedded with iPSCs were formed as previously described [13], except that here a chemically defined hydration fluid composed of buffered dextran was used. Briefly, as cells suspended in hydration fluid were added to the dry blend of hyaluronic acid and Chitosan under high agitation (vortexing), a gel formed instantly through polyelectrolytic complexation between HA COO‐ and CT NH3+ .…”