A Chlorination Method for Macerating Woody Tissues

Harlow, William M.

doi:10.1086/333836

Botanical Gazette

1928

DOI: 10.1086/333836

|View full text |Cite

A Chlorination Method for Macerating Woody Tissues

William M. Harlow¹

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1944

1964

Publication Types

Select...

Article5

Relationship

Self Cite0

Independent5

Authors

Journals

Cited by 5 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several maceration techniques for isolation of sclereids were followed. The chlorination method of Harlow (1928), using strong chlorine water plus 3% sodium sulphite; Jeffrey's (1917) method, using a mixture of equal parts of 10% chromic-nitric acids; and Kisser's J1926) method, using aqueous saturated picric acid followed by 3% hydrogen peroxide, were tried. Procedures of both Harlow and Jeffrey yielded fairly good results, but Kisser's method rendered the best maceration by preserving the wall intact as well as the protoplasmic structures of the sclereids.…”

mentioning

confidence: 99%

Ontogeny and Differentiation of Sclereids in Rauwolfia

Mia¹

1964

American J of Botany

View full text Add to dashboard Cite

An interesting anatomic feature of Rauwolfia is the occurrence of a remarkable type of sclereid in the stem and root. The initials of the sclereids in the stem arise in the ground tissue element of the pith in a region between 50 and 70μ below the surface of the shoot apex. This region of the shoot remains surrounded by a whorl of either 3 or 4 leaves. Sclereids initiate in succession in association with each whorl of leaves. Thus, the sclereids are restricted to the nodes. The sclereids in the stem arise as a primary element of the shoot from the ground tissue of the pith. In the root, they differentiate from the cells of the phelloderm and are secondary in origin. Morphologically, the sclereids in these 2 organs are basically the same, except that the sclereids in the stem are larger in size than those in the root. A solitary cell, or 2 to several cells in a longitudinal cell file (originated from a single mother cell), may differentiate into sclereid initials. The growth of the sclereids through relatively compact ground tissue of the pith is possibly accomplished by a combination of gliding growth and apical intrusive process. The sclereid initials grow rapidly and force their way between the parenchymatous cells. As a result, the neighboring cells lose their original surface contacts. Sclereids increase in size rapidly, and, therefore, very enlarged sclereids with thin primary walls may be observed in the second node. They mature progressively in basipetal direction in the subjacent nodes. In the fifth or sixth node, mature sclereids with massive secondary walls are most common. The secondary walls of sclereids contain much lignin as determined by the phloroglucinol‐HCl test. The walls of sclereids at maturity show a variable number of lamellae ranging from 10 to 15 in the lateral walls. A remarkable feature of the sclereids is their canal‐like pits in the secondary walls. Two adjacent pits may coalesce uniquely to form a Y‐like configuration directed centrifugally from the lumen of the sclereids. The sclereids are ventrically symmetrical, joined end‐to‐end by their transverse walls like 2 superimposed young fibers.

show abstract

mentioning

confidence: 99%

Ontogeny and Differentiation of Sclereids in Rauwolfia

Mia¹

1964

American J of Botany

View full text Add to dashboard Cite

show abstract

Dissociation des éléments histologiques du bois

Normand¹

1944

Bulletin de la Société Botanique de France

View full text Add to dashboard Cite

The Maceration of Woody Tissue With Acetic Acid and Sodium Chlorite

Spearin¹,

Isenberg²

1947

Science

View full text Add to dashboard Cite

Science, a weekly journal, is published each Friday by the American Association for the Advancement of Science at Mt. Royal & Guilford Avenues, Baltimore 2, Maryland. Founded in 1880, it has been since 1900 the official publication of the AAAS. Editorial, Advertising, and Circulation Offices, 1515 Massachusetts Avenue, N.W., Washington 5, D. C. Telephone, EXecutive 6060 or 6061. Articles offered for publication should be sent to the Editor, 1515 Massachusetts Avenue, N.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

A Chlorination Method for Macerating Woody Tissues

Cited by 5 publications

References 0 publications

Ontogeny and Differentiation of Sclereids in Rauwolfia

Ontogeny and Differentiation of Sclereids in Rauwolfia

Dissociation des éléments histologiques du bois

The Maceration of Woody Tissue With Acetic Acid and Sodium Chlorite

Contact Info

Product

Resources

About