A chondrocyte infiltrated collagen type I/III membrane (MACI® implant) improves cartilage healing in the equine patellofemoral joint model

Nixon, Alan J.; Rickey, Ellen J.; Butler, Tait; Scimeca, Michael S.; Moran, N.; Matthews, G.

doi:10.1016/j.joca.2014.12.021

Cited by 51 publications

(40 citation statements)

References 46 publications

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“…Stiffness tests in ovine models with a MACI type II collagen membrane for 1 year resulted in values 37–50% of native tissue . The same implant grown in an equine model reported compression values ranging between 40 and 70% of native tissue at 8 months and 1 year, respectively . Variability between in vivo animal models occur due to the type of animal and the repair technique.…”

Section: Discussionmentioning

confidence: 99%

“…40 The same implant grown in an equine model reported compression values ranging between 40 and 70% of native tissue at 8 months and 1 year, respectively. 27,41 Variability between in vivo animal models occur due to the type of animal and the repair technique. In contrast, after only 7 weeks of in vitro culture, our human constructs provide comparable or better results than multiple animal models grown for longer durations, thus providing similar or better load support.…”

Section: Mechanics Of Human Engineered Cartilagementioning

confidence: 99%

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Mechanical properties and structure‐function relationships of human chondrocyte‐seeded cartilage constructs after in vitro culture

Middendorf

Griffin

Shortkroff

et al. 2017

Journal Orthopaedic Research

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Autologous Chondrocyte Implantation (ACI) is a widely recognized method for the repair of focal cartilage defects. Despite the accepted use, problems with this technique still exist, including graft hypertrophy, damage to surrounding tissue by sutures, uneven cell distribution, and delamination. Modified ACI techniques overcome these challenges by seeding autologous chondrocytes onto a 3D scaffold and securing the graft into the defect. Many studies on these tissue engineered grafts have identified the compressive properties, but few have examined frictional and shear properties as suggested by FDA guidance. This study is the first to perform three mechanical tests (compressive, frictional, and shear) on human tissue engineered cartilage. The objective was to understand the complex mechanical behavior, function, and changes that occur with time in these constructs grown in vitro using compression, friction, and shear tests. Safranin-O histology and a DMMB assay both revealed increased sulfated glycosaminoglycan (sGAG) content in the scaffolds with increased maturity. Similarly, immunohistochemistry revealed increased lubricin localization on the construct surface. Confined compression and friction tests both revealed improved properties with increased construct maturity. Compressive properties correlated with the sGAG content, while improved friction coefficients were attributed to increased lubricin localization on the construct surfaces. In contrast, shear properties did not improve with increased culture time. This study suggests the various mechanical and biological properties of tissue engineered cartilage improve at different rates, indicating thorough mechanical evaluation of tissue engineered cartilage is critical to understanding the performance of repaired cartilage. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:2298-2306, 2017.

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Section: Discussionmentioning

confidence: 99%

Section: Mechanics Of Human Engineered Cartilagementioning

confidence: 99%

Mechanical properties and structure‐function relationships of human chondrocyte‐seeded cartilage constructs after in vitro culture

Middendorf

Griffin

Shortkroff

et al. 2017

Journal Orthopaedic Research

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“…Although the equilibrium modulus of our GF-tissue composite composed of murine ATDC5 cells was yet an order of magnitude lower than reported for the equilibrium modulus of adult human cartilage tissue measured using unconfined compression (0.24–0.85 MPa), [31] it was comparable to m-ACI solutions (~50 kPa) [49][50] and still shows promise in the area of guiding and improving cell differentiation as demonstrated in the current study by the increase in the compressive and equilibrium modulus of the GF-tissue composite with time in culture. In order to place our results in perspective, we compare the mechanical performance of our GF-tissue composites to recent results in the literature.…”

Section: Resultsmentioning

confidence: 83%

Mechanical Properties of Graphene Foam and Graphene Foam—Tissue Composites

et al. 2018

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Graphene foam (GF), a 3-dimensional derivative of graphene, has received much attention recently for applications in tissue engineering due to its unique mechanical, electrical, and thermal properties. Although GF is an appealing material for cartilage tissue engineering, the mechanical properties of GF – tissue composites under dynamic compressive loads have not yet been reported. The objective of this study was to measure the elastic and viscoelastic properties of GF and GF-tissue composites under unconfined compression when quasi-static and dynamic loads are applied at strain magnitudes below 20%. The mechanical tests demonstrate a 46% increase in the elastic modulus and a 29% increase in the equilibrium modulus after 28-days of cell culture as compared to GF soaked in tissue culture medium for 24h. There was no significant difference in the amount of stress relaxation, however, the phase shift demonstrated a significant increase between pure GF and GF that had been soaked in tissue culture medium for 24h. Furthermore, we have shown that ATDC5 chondrocyte progenitor cells are viable on graphene foam and have identified the cellular contribution to the mechanical strength and viscoelastic properties of GF – tissue composites, with important implications for cartilage tissue engineering.

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“…Collagen sponge wound coverings prevented foreign substances from entering the wound site, and promoted cellular infiltration at the injury site to facilitate new tissue growth and remodeling 13,14 . Most recently, FDA approval was granted for collagen membranes seeded with autologous chondrocytes for cartilage regeneration 15,16 . While the applications of collagen as sponges in tissue engineering are extensive, its uses in solid free-form fabrication or 3D printing applications to create hydrogels or sponges in defined 2D and 3D geometries is limited.…”

Section: Introductionmentioning

confidence: 99%

A thermoreversible, photocrosslinkable collagen bio-ink for free-form fabrication of scaffolds for regenerative medicine

et al. 2017

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As a biomaterial, collagen has been used throughout tissue engineering and regenerative medicine. Collagen is native to the body, is highly biocompatible, and naturally promotes cell adhesion and regeneration. However, collagen fibers and the inherent weak mechanical properties of collagen hydrogels interfere with further development of collagen as a bio-ink. Herein, we demonstrate the use of a modified type-I collagen, collagen methacrylamide (CMA), as a fibril-forming bio-ink for free-form fabrication of scaffolds. Like collagen, CMA can self-assemble into a fibrillar hydrogel at physiological conditions. In contrast, CMA is photocrosslinkable and thermoreversible, and photocrosslinking eliminates thermoreversibility. Free-form fabrication of CMA was performed through self-assembly of the CMA hydrogel, photocrosslinking the structure of interest using a photomask, and cooling the entire hydrogel, which results in cold-melting of unphotocrosslinked regions. Printed hydrogels had a resolution on the order of ~350 μm, and can be fabricated with or without cells and maintain viability or be further processed into freeze-dried sponges, all while retaining pattern fidelity. A subcutaneous implant study confirmed the biocompatibility of CMA in comparison to collagen. Free-form fabrication of CMA allows for printing of macroscale, customized scaffolds with good pattern fidelity and can be implemented with relative ease for continued research and development of collagen-based scaffolds in tissue engineering.

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A chondrocyte infiltrated collagen type I/III membrane (MACI® implant) improves cartilage healing in the equine patellofemoral joint model

Abstract: The MACI(®) implant appeared to improve cartilage healing in a critical sized defect in the equine model evaluated over 6 months.

Cited by 51 publications

References 46 publications

Mechanical properties and structure‐function relationships of human chondrocyte‐seeded cartilage constructs after in vitro culture

Mechanical properties and structure‐function relationships of human chondrocyte‐seeded cartilage constructs after in vitro culture

Mechanical Properties of Graphene Foam and Graphene Foam—Tissue Composites

A thermoreversible, photocrosslinkable collagen bio-ink for free-form fabrication of scaffolds for regenerative medicine

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