A Chronic Toxicity Study of Oral Administration of Collagen-?® Supplement using Pregnant Rabbits

Abstract

Since collagen derived products are highly used in food supplements due to its bioactive properties by influencing cellular and tissue health, flexibility with high effect of repairing and adaptation. It is necessary to carry out safety requirement of an oral chronic toxicity assessment. This study investigated the adverse effect of long term oral administration of collagen-® in pregnant rabbits. We have conducted a thirty days randomized controlled trial in pregnant rabbits. Rabbits were randomized into two groups; Group 1 (control group) were administrated with 1 ml of normal saline PO, SID while Group 2 (collagen-α group) were administered with 1 ml of collagen-α ® PO, SID for one month. Bodyweight gain, viscera weight and histopathological evaluation of the individual rabbit were recorded. The results revealed that the body weight gain and visceral tissues weight in the collagen-α group were significantly lower than the control group. Moreover, various histopathological changes were recorded in heart, lungs, liver and kidneys of the collagen-α group when compared with the non-significant changes that noticed in the control group. Under the conditions of the current study, a high risk of chronic toxic effect was observed in pregnant rabbits inoculated with 1 ml of oral dose of collagen alpha supplement. This result suggests that the adverse effect of collagen derived supplements is overbalancing the benefits.

Evaluation the Effect of Oral Collagen-Α® Supplementation on the Hepatic and Renal Functions in Mature Male Rabbits

“…In this study, we reported decreased in liver and kidneys weights, these may be due to its acts as anti-obesity by it regulation metabolism of lipids and proteins and also may be suggested it effects on appetite centers in the brain (20). These changes in the liver, and kidneys weights agreement with that reported by Kudayeret al (9) who noted that used 1 ml of CH-α® supplementation for pregnant rabbits for 30 days have adverse effect on liver and kidney weights and its functions and also signed to changes negatively in body weight and BW gain. The histological changes recorded in figures of liverand kidneys related to the changes in body, organs weights and BW changes and similar to that reported by Kudayeret al (9) that notes increase in elasticity of muscles but decrease in muscle mass.…”

“…These changes in the liver, and kidneys weights agreement with that reported by Kudayeret al (9) who noted that used 1 ml of CH-α® supplementation for pregnant rabbits for 30 days have adverse effect on liver and kidney weights and its functions and also signed to changes negatively in body weight and BW gain. The histological changes recorded in figures of liverand kidneys related to the changes in body, organs weights and BW changes and similar to that reported by Kudayeret al (9) that notes increase in elasticity of muscles but decrease in muscle mass. Like other types of animal protein, collagen contains a type of amino acid called hydroxyproline (21).…”

“…Collagen-α source and dosage: each ampulla contain 10 ml of Collagen Peptide (5g), Rosehip Extract (0.5g) and Vitamin-C (60mg), dosage 1ml/kg BW) (10).…”

“…It has been reported that CH can improve glucose tolerance by inhibiting intestinal glucose uptake and enhancing insulin secretion, suggesting the antidiabetic property of CH (7).Previous studies have revealed that CH-II reduced lameness pain and pain during limb manipulation, and physical exertion in arthritic (8). A high risk of chronic toxic effect was observed in pregnant rabbitsinoculated with 1 ml of oral dose of collagen alpha supplement (9).The current study was designed to evaluate the effects of Collagen-α® as a dietary supplement on hepatic and renal functional changes in healthy adult male rabbits.…”

“…Fig (6) histological section of kidney of group A (control) showed normal architecture of renal parenchyma, Fig(7) Histological section in kidney group B(CH-α®15) showed hyperplasia of mesangial cells and moderate vacuolation of renal tubules, Fig (8) Histological section in kidney in group B (CH-α®15) showed atrophy of glomerulus, in addition to moderate vacuolation and dilation renal tubules. Histological section in kidney group C (CH-α®30) showed in fig (9) appeared the glomerular atrophy and dilation of glomerular space and vacuolation of renal tubules while Fig(10) showed the histological section of kidney in G C (CH-α®30) showed mesangial cells hyperplasia.…”