A CLA Mixture Prevents Body Triglyceride Accumulation without Affecting Energy Expenditure in Syrian Hamsters

Abstract

We examined the effects of feeding conjugated linoleic acids (CLA) to adult male hamsters on several components of energy metabolism and body composition. Hamsters (n = 54) were assigned for 6-8 wk to one of three diets: 1) a standard diet (in percentage energy: lipids, 33, carbohydrates, 49, and proteins, 18); 2) to the standard diet augmented with the 9c,11t-isomer of CLA to 1.6% of energy (R group); or 3) the standard diet augmented with the 9c,11t-isomer and the 10t,12c-CLA isomer to 3.2 (1.6 + 1.6) % of energy (CLA mix group). (15)N uniformly labeled milk-protein was included in the diet to measure the incorporation of dietary protein into liver and muscle. Basal metabolic rate, thermogenic response to feeding and energy expenditure during spontaneous activity or during an exercise at approximately 60% of VO(2max) were measured. Carnitine palmitoyltransferase-I (CPT-I), leptin, insulin and triiodothyronine concentrations, as well as the in vivo overall adiposity changes were also determined. After 6 wk, the whole-body triglyceride content determined in vivo by NMR was significantly higher in the R group than in the control and CLA mix groups. The CLA mix group differed from the others in the lack of body triglyceride accumulation between d 21 and d 45 of the study, and the appearance of a slight insulin-resistance (homeostatic model assessment index, P < 0.05). Paradoxically, the lack of effect on whole-body lipid oxidation was associated with a greater CPT-I-specific activity in tissues of both CLA-fed groups (P < 0.05). No other major effects of CLA feeding were detected. In conclusion, CLA supplementation in hamsters did not affect adipose weight or the components of energy expenditure despite a theoretically higher capacity of red muscle to oxidize lipids. Only a CLA mixture prevented whole-body triglyceride accumulation over time.

Effects of intracerebroventricular and intra-accumbens melanin-concentrating hormone agonism on food intake and energy expenditure

“…Such an increase was not seen in pair-fed rats and was accompanied with an increase in Gox and a reduction in Lox. Food intake per se is known to increase Gox and to reduce Lox (8). The pattern of substrate oxidation seen in rats given the MCH agonist in the NAcSh was similar to that seen following the intracerebroventricular administration of the peptide.…”

Milk consumption and acne in teenaged boys

“…23 Animals fed α-lactalbumin-enriched whey protein show increased will and capacity to engage in physical activities, gains in lean body mass, improved efficiency of exercise training, and decreased percentage body fat mass; all of which are similar to the effect of androgens. [24][25][26] In addition, α-lactalbumin undergoes pressure-induced conformational alteration, possibly as a result of centrifugation stresses during processing, that leads to changes in biological function. 27 Our finding of a definite but weak association between milk intake and acne in boys contrasts markedly with our findings in girls 7,8 but nonetheless provides support for this hypothesis.…”

Influence of dietary conjugated linoleic acid on growth, fatty acid composition and hepatic lipogenesis in large yellow croaker (Pseudosciaena crocea R.)

“…Dietary CLA has some beneficial effects on body composition in mammals; decreased body fat and increased lean body mass were observed in mice (Ohnuki et al, 2001;Terpstra et al, 2002), rats (Yamasaki et al, 2003) and pigs (Thiel-Cooper et al, 2001;Tischendorf et al, 2002). CLA also decreased whole body triacylglycerol (TAG) accumulation in hamsters (Bouthegourd et al, 2002) and reduced liver TAG levels in rats (Rahman et al, 2002). However, we did not observe the similar effects in large yellow croaker in the present trail, which agrees with the redults from a few previous studies, such as the findings in salmon fry (Berge et al, 2004), rainbow trout juveniles (Figueiredo-Silva et al, 2005), and smolts (Kennedy et al, 2005).…”