A Clinical Study Evaluating Changes in the Microbial Flora Around Dental Implants During Various Stages of Implant Restoration

Herekar, Manisha; Sethi, Megha; Prithviraj, Doddammane R.; Bhat, Kishore; Fernandes, Aquaviva; Patil, Viraj

doi:10.1097/id.0000000000000273

Cited by 8 publications

(13 citation statements)

References 26 publications

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“…Hereker et al reported similar bacterial load in nonadherent and adherent plaque samples around dental implants. 13 The loads of various bacteria changed but not significantly during the follow-up period of 1 month, as found in the study. Harder et al reported mean bacterial load to be 41 × 10 4 CFU/mL in nonadherent samples only.…”

Section: Discussionsupporting

confidence: 66%

“…Similar findings were reported by van Winkelhoff et al 19 Hereker et al also reported very less prevalence (1%) of P. gingivalis in their study. 13 The P. intermedia group bacteria are black-pigmented gram-negative anaerobic rods that are commonly found in Bacterial Identification and Monitoring Around Two-Piece Dental Implants Saha et al…”

Section: Discussionmentioning

confidence: 99%

“…Hereker et al also reported extremely low prevalence of P. intermedia in their study using conventional identification methods. 13 Veillonella spp. (V. parvula and V. atypica) is a gram-negative oral bacteria.…”

Section: Discussionmentioning

confidence: 99%

“…All isolates were subjected to various microbiological methods for identification. Organisms were quantified in colony-forming units (CFU) per milliliter for each sample tested according to Hereker et al 13 The bacterial isolates were first stained by Gram staining to differentiate between gram-positive bacteria and gram-negative bacteria. The identities were further confirmed by biochemical tests as given by Dowell and Hawkins.…”

Section: Methodsmentioning

confidence: 99%

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Bacterial Identification and Monitoring Around Two-Piece Dental Implants by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS)

et al. 2020

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Context Early microbiological diagnosis and treatment of periodontal pathogens is important for successful retention of dental implants. Aims This study aimed to identify and monitor oral bacterial colonization after successful two-piece dental implants. Settings and Design In this study, 50 two-piece dental implant subjects were included and assessed clinically, radiographically, and microbiologically. Methods and Material All the parameters were recorded at various stages after prosthesis placement. In each stage, nonadherent (peri-implant sulcular fluid) and adherent (curetted inner threads) samples were collected. Semiquantitative anaerobic culture of the samples were done in Anoxomat system. Bacterial colonies were first identified by routine microbiological methods and then by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) method. Statistical Analysis All the results were analyzed by appropriate statistical methods (Chi-square, one factor analysis of variance, etc.). Results All the bacterial isolates were identified in the MALDI-TOF MS system with no failure. After implant placement for the nonadherent samples, the frequency (%) of Fusobacterium nucleatum, Prevotella melaninogenica, and Propionibacterium acnes decreased whereas frequency (%) of Escherichia coli, Staphylococcus epidermidis, and Streptococcus mitis increased. For adherent samples, the frequency (%) of E. coli, Enterococcus faecalis, Porphyromonas gingivalis, P. melaninogenica, and Veillonella parvula decreased, whereas frequency (%) of S. mitis and Streptococcus mutans increased. The postimplant mean nonadherent and adherent bacterial load increased with time but not significantly over the periods (p = 0.302 and 0.123, respectively). Conclusion Combination of basic (semiquantitative culture method) and advanced microbiological method (MALDI-TOF MS) can be useful for accurate detection and monitoring of potential pathogens around two-piece dental implants.

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Bacterial Identification and Monitoring Around Two-Piece Dental Implants by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS)

et al. 2020

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“…This microorganism has been reported to dominate the biofilm community (27), colonize the microbial flora around dental implants (28), be present into the saliva and pooled subgingival plaque samples of aggressive periodontitis (29) and Fanconi's anemia patients (30). Toothpaste that contains of mastic is available in Japan.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Biological Activity of Mastic Extracts Based on Chemotherapeutic Indices

Suzuki

Sakagami

Amano

et al. 2017

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Abstract. Background: Most previous mastic investigatorshave not Mastic is the extract of sap from Pistacia lentiscus, grown only in the Chios island of Greece. Due to its unique shape and diverse efficacy, mastic has been called "the tear drop of Christ". Mastic extracts have been reported to show antitumor activity (including the enhancement of anticancer drugs' action and induction of apoptosis via oxidative stress) (1, 2), antioxidant activity (that correlated with phenolic and flavonoid contents) (3-8), antibacterial activity (9-11), modulating activity of drug-metabolizing enzymes (12, 13) and antiviral activity (14-17). However, most previous investigators did not describe the chemotherapeutic index of these activities (that is the ratio of the biological activity to cytoxicity), even though there is a cautionary note that mastic extracts showed potent cytotoxicity (18).In the present study, we separated the crude mastic extract into 5 different fractions using organic solvents and, then, reexamined the biological activity of unfractionated and fractioned extracts, based on chemotherapeutic indexes. Materials and MethodsMaterials. The following chemicals and reagents were obtained from the indicated companies: Dulbecco's modified Eagle's medium (DMEM) (Gibco BRL, Grand Island, NY, USA); fetal bovine serum (FBS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), RPMI 1640 medium, doxorubicin, azidothymidine (AZT), 2',3'-dideoxycytidine (ddC) (Sigma-Aldrich Inc., St. Louis, MO, USA); dimethyl sulfoxide (DMSO) (Wako Pure Chemical Ind., Ltd., Osaka, Japan), curdlan sulfate (CRDS) (79 kDa; Ajinomoto Co. Inc., Tokyo, Japan). Culture plastic dishes and plates (96-well) were 591 This article is freely accessible online.

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Microbiological Factors of Peri-Implantitis: Characteristics and Significance

Saito

Kensara

Masri

2020

Risk Factors for Peri-Implant Diseases

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A Clinical Study Evaluating Changes in the Microbial Flora Around Dental Implants During Various Stages of Implant Restoration

Abstract: Regular microbial evaluation along with clinical and radiographic monitoring could help in recognizing the potential for periimplantitis and in prevention of the same.

Cited by 8 publications

References 26 publications

Bacterial Identification and Monitoring Around Two-Piece Dental Implants by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS)

Bacterial Identification and Monitoring Around Two-Piece Dental Implants by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS)

Evaluation of Biological Activity of Mastic Extracts Based on Chemotherapeutic Indices

Microbiological Factors of Peri-Implantitis: Characteristics and Significance

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