A Closed-system Technology for Mechanical Isolation of High Quantities of Stromal Vascular Fraction from Fat for Immediate Clinical Use

Abstract

Background: Adipose tissue stromal vascular fraction (SVF) is increasingly used in the clinic. SVF separation from fat by enzymatic disruption is currently the gold standard for SVF isolation. However, enzymatic SVF isolation is time-consuming (~1.5 h), costly and significantly increases the regulatory burden of SVF isolation. Mechanical fat disruption is rapid, cheaper, and less regulatory challenging. However, its reported efficacy is insufficient for clinical use. The current study evaluated the efficacy of a novel rotating blades (RBs) mechanical SVF isolation system. Methods: SVF cells were isolated from the same lipoaspirate sample (n = 30) by enzymatic isolation, massive shaking (wash), or engine-induced RBs mechanical isolation. SVF cells were counted, characterized by flow cytometry and by their ability to form adipose-derived stromal cells (ASCs). Results: The RBs mechanical approach yielded 2 × 10 5 SVF nucleated cells/mL fat, inferior to enzymatic isolation (4.17 × 10 5 ) but superior to cells isolating from fat by the "wash" technique (0.67 × 10 5 ). Importantly, RBs SVF isolation yield was similar to reported yields achieved via clinical-grade enzymatic SVF isolation. RBs-isolated SVF cells were found to contain 22.7% CD45 -CD31 − CD34 + stem cell progenitor cells (n = 5) yielding quantities of multipotent ASCs similar to enzymatic controls. Conclusions: The RBs isolation technology provided for rapid (<15 min) isolation of high-quality SVF cells in quantities similar to those obtained by enzymatic digestion. Based on the RBs platform, a closed-system medical device for SVF extraction in a rapid, simple, safe, sterile, reproducible, and costeffective manner was designed.

Advanced methods to mechanically isolate stromal vascular fraction: A concise review

“…The current trend in preparing mSVF products is to combine several basic physical methods to achieve simplicity, short processing time, and high efficiency. For instance, the Solodeev team recently developed a closed, integrated, fully automated mSVF extraction device that integrates washing, filtration, and cutting processes in just 15 min, without the need for additional processing [ 38 ]. Furthermore, innovative techniques, like microfluidics technology, have emerged.…”