A co-housing strategy to improve fecundity of mice in timed matings

Stiles, Robert J; Schrum, Adam G.; Gil, Diana

doi:10.1038/laban.161

Cited by 8 publications

(7 citation statements)

References 13 publications

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“…Results are given as the mean AE standard error of the mean (SEM). effect with co-housing as suggested by Stiles et al 7 Surprisingly, even without using such approaches, we observed more plug-positive females after overnight mating than expected. Whitten 8 showed that only 13-14% of young virgin females had a vaginal plug on the first two days after mating.…”

Section: Discussionsupporting

confidence: 71%

Efficiency of timed pregnancies in C57BL/6 and BALB/c mice by mating one male with up to four females

2020

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For a wide range of biomedical approaches, an accurate estimate of the age of embryos or pups is important. Overnight mating is the method that is mostly used to establish timed pregnancies. The oestrus cycle in mice repeats every four to five days. So, not all females will get pregnant because they are not in oestrus. Therefore, the aim of this study was to analyse whether polygamous mating could increase the rate of timed pregnancies per breeding cage and female. We compared overnight timed mating regimes with up to four females per male, using C57BL/6 and BALB/c mice as well as F1 hybrids of these two strains. The number of vaginal plugs, number of females that gave birth and weaned litter (including size and weaning weight) were recorded. Our results showed that the plug and pregnancy rate decreased, but the productivity per breeding cage increased for polygamous mating regimes. The proportion of females with vaginal plugs and females that gave birth was significantly higher in monogamous mating. The proportion of plugged females that gave birth, as well as litter size and weaning weight, were not influenced by the mating regime. After analysing 513 breeding cages with a total of 1090 females, we found that polygamous mating with up to three females per male can increase the number of timed pregnancies. However, in the mating regime with more than three females, the rate of timed pregnancy as well as number of pups per female declined.

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Section: Discussionsupporting

confidence: 71%

Efficiency of timed pregnancies in C57BL/6 and BALB/c mice by mating one male with up to four females

2020

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“…To the Group IV animals, 10 ppm of Hg Chloride was administered and Group V and VI were administrated 10 ppm of HgCl 2 along with 150 and 300 ppm of Curcumin. On the day of pregnancy the animals were divided into three subgroups and the experiments were carried out as described by Stiles et al (2013).…”

Section: Methodsmentioning

confidence: 99%

Neurobehavioral protective properties of curcumin against the mercury chloride treated mice offspring

Abu-Taweel

2019

Saudi Journal of Biological Sciences

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In the present investigation, the effects of mercuric chloride (HgCl 2 ) on the neurobehavioural and neurochemical disruption in mice offspring was studied. A total of thirty pregnant mice were divided into six groups. Group II and III were received 150 and 300 ppm of curcumin respectively. Group IV was given 10 ppm of HgCl 2 . Group V and VI were given 10 ppm of HgCl 2 with 150 and 300 ppm of curcumin respectively. In this study, treatment started from day one of pregnancy and continued until post-natal day 15 (PD 15). During weaning period, three pups in each experimental group were marked and were subjected to behavioral, physical and biochemical tests. The results revealed decreased body weight, delayed hair growth and eye opening. HgCl 2 treated pups taken more time in righting, rotating reflexes to return to normal placement, cliff avoidance compared to that of control group. HgCl 2 exposed pups showed memory and learning deficits. Anxiety behavior in treating pups was increased. Biochemical investigations showed decreased level of dopamine (DA), serotonin (5-HT) and acetylcholinesterase (AChE) in forebrain of treated pups compared to the control and curcumin groups. The protective effect of curcumin doses were significant compared to HgCl 2 group. The results indicated that the administration of curcumin showed effective activity towards biochemical and behavioral disorders obtained with the HgCl 2 treated animals. Overall, the curcumin administration revealed increased cognetion and anxiety behaviors in the treated animals. Conclusively, curcumin has a good benefits for health which can use to avoid toxicants such as Hg and other heavy metals.

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“…To improve the success of timed matings when female and male mice are housed together for a limited time, breeding pairs can be maintained in the same box separated by a transparent partition for 3 days before setting up the timed mating. This permits male urine-borne pheromones to induce female estrus, thereby enabling the expression of male and female mating behaviors ( Stiles et al, 2013 ).…”

Section: Effects Of Environment On Ethologically Appropriate Behaviormentioning

confidence: 99%

Living inside the box: environmental effects on mouse models of human disease

Sundberg

Schofield

2018

Disease Models &Amp; Mechanisms

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The impact of the laboratory environment on animal models of human disease, particularly the mouse, has recently come under intense scrutiny regarding both the reproducibility of such environments and their ability to accurately recapitulate elements of human environmental conditions. One common objection to the use of mice in highly controlled facilities is that humans live in much more diverse and stressful environments, which affects the expression and characteristics of disease phenotypes. In this Special Article, we review some of the known effects of the laboratory environment on mouse phenotypes and compare them with environmental effects on humans that modify phenotypes or, in some cases, have driven genetic adaptation. We conclude that the ‘boxes’ inhabited by mice and humans have much in common, but that, when attempting to tease out the effects of environment on phenotype, a controlled and, importantly, well-characterized environment is essential.

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A co-housing strategy to improve fecundity of mice in timed matings

Cited by 8 publications

References 13 publications

Efficiency of timed pregnancies in C57BL/6 and BALB/c mice by mating one male with up to four females

Efficiency of timed pregnancies in C57BL/6 and BALB/c mice by mating one male with up to four females

Neurobehavioral protective properties of curcumin against the mercury chloride treated mice offspring

Living inside the box: environmental effects on mouse models of human disease

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