A Co-operative Numerical Analysis of Scotochromogenic Slowly Growing Mycobacteria

Wayne, Lawrence G.; Dietz, Thomas; Gernez-Rieux, C; Jenkins, P. A.; Käppler, W; Kubica, George P.; Kwapinski, J; Meißner, Gertrud; Pattyn, S. R.; Runyon, Ernest H.; Schröder, K. H.; Silcox, Vella A.; Tacquet, A; Tsukamura, M; Wolinsky, Emanuel

doi:10.1099/00221287-66-3-255

Cited by 47 publications

(29 citation statements)

References 16 publications

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“…This is a report on that study, in which investigators from 18 different laboratories (coded A to R) participated. The investigation was carried out under the permissive philosophy and the other ground rules presented in the report of the prior study on scotochromogenic mycobacteria (Wayne et al 1971).…”

Section: G M E I S S N E R a N D O T H E R Smentioning

confidence: 99%

A Co-operative Numerical Analysis of Nonscoto- and Nonphotochromogenic Slowly Growing Mycobacteria

Meißner

Schröder

et al. 1974

Journal of General Microbiology

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A co-operative taxonomic study has been performed on slowly growing nonpigmented mycobacteria (Run yon's group 111). Phenetic data on 89 strains, studied in 18 laboratories, were collected and analysed by a numerical taxonomic method. A variety of immunological properties, lipid analyses and measures of pathogenicity were analysed independently to establish correlation with numerical classification. Mycobacterium gastri, M . nonchromogenicum, M . terrae, M . avium and M . xenopi were recognized by almost all participants as distinct species. Mycobacterium novum was considered to be synonymous with M . terrae. A clearcut distinction could not be made between M . avium and M. intracellulare; the majority of participants in the study recommend that M . intracellulare be reduced to a synonym of M . avium. A minority of authors cannot agree with this proposal.

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Section: G M E I S S N E R a N D O T H E R Smentioning

confidence: 99%

A Co-operative Numerical Analysis of Nonscoto- and Nonphotochromogenic Slowly Growing Mycobacteria

Meißner

Schröder

et al. 1974

Journal of General Microbiology

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“…(40 cultures). All cultures were lyophilized, coded and distributed by the ATCC as before (Wayne et al 1971).…”

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confidence: 99%

“…Participants studied the cultures using tests of their own choosing. As before (Wayne et al 1971)~ participants agreed not to withdraw their data regardless of the final result and agreed that no details of the techniques used would be presented at this stage. The individual batches of data were sent to the co-ordinators who recorded the relevant information into binary form for the numerical analyses.…”

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confidence: 99%

A Co-operative Numerical Analysis of Cultures Considered to Belong to the 'rhodochrous' Taxon

Goodfellow

Lind

Mordarska

et al. 1974

Journal of General Microbiology

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SUMMARYA co-operative taxonomic study has been performed on cultures belonging to the ' rhodochrous complex '. Phenetic data on 98 cultures (78 rhodochrous cultures, 12 marker cultures with the genus designation Mycobacterium and 8 with the designation Nocardia) studied in four laboratories were collected and analysed by numerical taxonomic methods. The precipitinogenic properties and the presence of different types of mycolic acids were analysed independently to establish correlation with the numerical classification. The chemotaxonomic and serological data correlated well with the numerical analyses. The rhodochrous taxon can be distinguished from the genera Mycobacterium and Nocardia, and from the proposed genus Actinomadura; furthermore, it can be divided into at least three homogeneous subgroups. Further studies however, are needed before the question of the generic location of the rhodochrous taxon can be settled and before the taxonomic status of the rhodochrous subclusters can be resolved.

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“…paraffinicum' strain ATCC 12670 5IWGMT OES 90139. An early study of slowly growing scotochromogenic mycobacteria of Runyon's group II used 140 characters to develop an n6n matrix table to demonstrate clustering behaviour of the study species (Wayne et al, 1971). The IWGMT noted that cluster 4 included Mycobacterium scrofulaceum ATCC 19981 T and 'M.…”

mentioning

confidence: 99%

Revival and emended description of ‘Mycobacterium paraffinicum’ Davis, Chase and Raymond 1956 as Mycobacterium paraffinicum sp. nov., nom. rev.

Toney

Adékambi

Toney

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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The omission of the name 'Mycobacterium paraffinicum' from the Approved Lists of Bacterial Names was due to phenotypic confusion surrounding a close relationship with Mycobacterium scrofulaceum. Correspondingly, 'M. paraffinicum' strains grew slowly in .7 days, stained acidalcohol-fast and produced yellow-pigmented, smooth, waxy colonies in the dark at an optimal temperature of 35 6C. However, 'M. paraffinicum' strains demonstrated no activity for urease, nicotinamidase or pyrazinamidase and lacked growth at 42 6C, unlike M. scrofulaceum. The mycolic acid pattern, as determined by HPLC, clustered 'M. paraffinicum' with M. scrofulaceum, Mycobacterium avium and Mycobacterium parascrofulaceum. Strains were fully susceptible to linezolid, rifabutin, clarithromycin and amikacin. Examination of the historical reference strain of 'M. paraffinicum', ATCC 12670, and five additional isolates using comparative studies with 16S rRNA, hsp65 and rpoB gene and concatenated sequences showed that they formed a tight taxonomic group that was distinct from similar non-tuberculous mycobacteria. Multilocus enzyme electrophoresis (MEE) analysis confirmed a close association of the five additional isolates with the reference strain of 'M. paraffinicum' with a genetic distance of 0.12 and showed that all six strains were distinct from other closely related species. These genetic results provided unambiguous evidence of the uniqueness of this slowly growing, scotochromogenic species and supported the revival of the name as Mycobacterium paraffinicum (ex Davis, Chase and Raymond 1956) sp. nov., nom. rev. We propose the previously deposited reference strain ATCC 12670 T 5DSM 44181 T 5NCIMB 10420 T , located in collections worldwide, as the type strain.In 1956, multiple strains of a soil bacterium were isolated below the level of grass roots in oily pools. They were characterized as Gram-positive, acid-alcohol-fast, nonmotile, rod-shaped bacilli, with white colonies that became yellow with age and were waxy and wrinkled on selective isolation medium of mineral salts agar in a gaseous atmosphere of 40 % ethane in air. Subsequently, the bacteria were grown on ethanol-and acetate-containing medium and the name 'Mycobacterium paraffinicum' was suggested to represent a paraffinic, gaseous hydrocarbonutilizing, non-tuberculous mycobacterium (NTM). The proposed name was 'meant to connote the general utilization of gaseous and liquid paraffinic hydrocarbons' (Davis et al., 1956). Furthermore, these mycobacteria were abundant in soil samples of petroliferous emanation, principally oilfields, and demonstrated specificity for ethane and higher paraffinic hydrocarbons (Davis et al., 1959). The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, hsp65 and rpoB gene sequences of isolates 90683, 90750, 30760, 70769 and 80776 and 'M. paraffinicum' ATCC 12670 are respectively GQ153265-GQ153270 (16S rRNA gene), GQ153283-GQ153288 (hsp65) and GQ153299-GQ153304 (rpoB).A comparison of mycolic acid patterns, a dendrogram based on ETs and neighbour-joining tree...

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A Co-operative Numerical Analysis of Scotochromogenic Slowly Growing Mycobacteria

Cited by 47 publications

References 16 publications

A Co-operative Numerical Analysis of Nonscoto- and Nonphotochromogenic Slowly Growing Mycobacteria

A Co-operative Numerical Analysis of Nonscoto- and Nonphotochromogenic Slowly Growing Mycobacteria

A Co-operative Numerical Analysis of Cultures Considered to Belong to the 'rhodochrous' Taxon

Revival and emended description of ‘Mycobacterium paraffinicum’ Davis, Chase and Raymond 1956 as Mycobacterium paraffinicum sp. nov., nom. rev.

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