A colorful approach towards developing new nano-based imaging contrast agents for improved cancer detection

Salinas, Helen R.; Miyasato, Dominie; Eremina, Olga E.; Perez, Rodolfo; Gonzalez, Karen L.; Czaja, Alexander; Burkitt, Sean; Aron, Arjun; Fernando, Augusta; Ojeda, Lauro S.; Larson, Kimberly N.; Mohamed, Ahmed W.; Campbell, Jos L.; Goins, Beth; Zavaleta, Cristina

doi:10.1039/d0bm01099e

Cited by 14 publications

(12 citation statements)

References 64 publications

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“…Figure d shows a schematic of SERS nanoparticles used for imaging. Due to the LSPR effect of AuNPs, the intensity of Raman signals for adsorbed molecules is enhanced dramatically, allowing them to be detected at extremely low concentrations . Some groups have utilized near-infrared (NIR) resonant Raman dyes to provide additional enhancement when utilized with a NIR laser, , but these resonant SERS NPs exhibit a high-fluorescence background and crowded Raman spectra, which are both properties known to negatively impact the conditioning of a multiplexed system and contribute to degraded performance at higher plexities.…”

Section: Resultsmentioning

confidence: 99%

Expanding the Multiplexing Capabilities of Raman Imaging to Reveal Highly Specific Molecular Expression and Enable Spatial Profiling

et al. 2022

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Profiling the heterogeneous landscape of cell types and biomolecules is rapidly being adopted to address current imperative research questions. Precision medicine seeks advancements in molecular spatial profiling techniques with highly multiplexed imaging capabilities and subcellular resolution, which remains an extremely complex task. Surface-enhanced Raman spectroscopy (SERS) imaging offers promise through the utilization of nanoparticle-based contrast agents that exhibit narrow spectral features and molecular specificity. The current renaissance of gold nanoparticle technology makes Raman scattering intensities competitive with traditional fluorescence methods while offering the added benefit of unsurpassed multiplexing capabilities. Here, we present an expanded library of individually distinct SERS nanoparticles to arm researchers and clinicians. Our nanoparticles consist of a ∼60 nm gold core, a Raman reporter molecule, and a final inert silica coating. Using density functional theory, we have selected Raman reporters that meet the key criterion of high spectral uniqueness to facilitate unmixing of up to 26 components in a single imaging pixel in vitro and in vivo. We also demonstrated the utility of our SERS nanoparticles for targeting cultured cells and profiling cancerous human tissue sections for highly multiplexed optical imaging. This study showcases the far-reaching capabilities of SERS-based Raman imaging in molecular profiling to improve personalized medicine and overcome the major challenges of functional and structural diversity in proteomic imaging.

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Section: Resultsmentioning

confidence: 99%

Expanding the Multiplexing Capabilities of Raman Imaging to Reveal Highly Specific Molecular Expression and Enable Spatial Profiling

et al. 2022

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“…Furthermore, novel imaging agents with better sensitivity and specificity can improve early detection during routine screening and help with tumor margin visualization during surgical resection. Recently, optical properties were investigated for multiple dyes and pigments used in tattoo inks, foods, drugs, and cosmetics already FDA approved [306]. Absorption, fluorescence, and Raman scattering properties were evaluated, and several exhibit a multitude of useful optical properties, outperforming some of the clinically approved imaging dyes on the market.…”

Section: The Future Of Cancer Diagnostics and Imagingmentioning

confidence: 99%

Cancer nanotechnology: current status and perspectives

2021

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Modern medicine has been waging a war on cancer for nearly a century with no tangible end in sight. Cancer treatments have significantly progressed, but the need to increase specificity and decrease systemic toxicities remains. Early diagnosis holds a key to improving prognostic outlook and patient quality of life, and diagnostic tools are on the cusp of a technological revolution. Nanotechnology has steadily expanded into the reaches of cancer chemotherapy, radiotherapy, diagnostics, and imaging, demonstrating the capacity to augment each and advance patient care. Nanomaterials provide an abundance of versatility, functionality, and applications to engineer specifically targeted cancer medicine, accurate early-detection devices, robust imaging modalities, and enhanced radiotherapy adjuvants. This review provides insights into the current clinical and pre-clinical nanotechnological applications for cancer drug therapy, diagnostics, imaging, and radiation therapy.

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“…Therefore, these compounds could attain low enhancement coefficients if using only SERS. Thus, the determination of phenolic compounds that are not dyes 46 and do not possess any functional groups anchoring to the noble metal nanoparticles 47 using SERS spectroscopy seems to be ineffective. As a result of the discrepancy between the optical absorption range of these compounds, the laser excitation wavelengths in SERS experiments, and the plasmon resonance band of the metal material, the "chemical enhancement" mechanism 48 of SERS becomes negligible.…”

Section: Determination Of Phenolic Compounds Via Sersmentioning

confidence: 99%

Ultrasensitive and multiplex SERS determination of anthropogenic phenols in oil fuel and environmental samples

Eremina

Kapitanova

Ferree

et al. 2022

Environ. Sci.: Nano

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A colorful approach towards developing new nano-based imaging contrast agents for improved cancer detection

Abstract: Coloring dyes reveal potential as optical imaging contrast agents for cancer detection. Tattoo and Design created by artist Adam Sky.

Cited by 14 publications

References 64 publications

Expanding the Multiplexing Capabilities of Raman Imaging to Reveal Highly Specific Molecular Expression and Enable Spatial Profiling

Expanding the Multiplexing Capabilities of Raman Imaging to Reveal Highly Specific Molecular Expression and Enable Spatial Profiling

Cancer nanotechnology: current status and perspectives

Ultrasensitive and multiplex SERS determination of anthropogenic phenols in oil fuel and environmental samples

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