A Colorimetric Method for the Determination of Dissolved Oxygen in Beer

Progress in Brewing Science and Technology

“…Beginning with a paper in 1936 (54) on the simultaneous determination of carbon dioxide and air in beer, a series of papers has been published by the authors' laboratories dealing with various aspects of measuring and controlling oxidation in beer (55,63,64,66,67,150,194,200). References 55,58,69,62,64,[66][67][68]150,172,174,192,194,200, together with other papers on the general subject of beer stability, have been collected in book form under the title "Bottle Beer Quality. A 10-Year Research Record at Wallerstein Laboratories," published in 1948.…”

“…Efforts to reduce opportunities for oxidation during cellaring must also be considered. One of the papers (150) in the series from the authors' laboratories already referred to deals with a method for measuring colorimetrically the amount of dissolved oxygen in beer sampled in the tank. Measurement of dissolved air in tank beer has also become an essential control procedure.…”

Quantitative Determination of Dissolved Oxygen: Ascorbic Acid Oxidase Method

“…The oxygen content in milligrams per liter of milk is obtained by multiplying the difference in dye titration before and after adding the ascorbic acid oxidase by twice the dye factor divided by 11. The dye factor is multiplied by 2 because only 5 ml. of milk were titrated and divided by 11 because the molecular weight of ascorbic acid is eleven times the atomic weight of oxygen.…”

“…17) method is of this type. These methods are not applicable to biological fluids because the reagents are relatively strong, are not specific for oxygen, and react with organic material.Methods have been described based on the reaction between dissolved oxygen and any one of a series of reducing agents to produce a colored compound, the amount of which is determined colorimetrically (9,11,18). Alkalies or acids are used to accelerate the reaction.…”

“…Methods have been described based on the reaction between dissolved oxygen and any one of a series of reducing agents to produce a colored compound, the amount of which is determined colorimetrically (9,11,18). Alkalies or acids are used to accelerate the reaction.…”