1954
DOI: 10.1042/bj0560639
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A colorimetric micro-method for the determination of glucose

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1955
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Cited by 130 publications
(54 citation statements)
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“…The methods of Mendel, Kemp and Myers ( 1954) and Kemp and Kits van Heijningen (1954) were used for the determination of carbohydrate, the animals being first ground with a mixture of 5% trichloracetic acid, containing 0.1% Ag2S04, and the mixture then boiled for 15 min. After cooling and centrifuging, 1 ml of the supernatant liquid was boiled for 61/z min with 3 ml concentrated H2S04 ( 1.84 specific gravity), The intensity of the resulting pink color was then determined by a Beckman D.U.…”
Section: Meteiodsmentioning
confidence: 99%
“…The methods of Mendel, Kemp and Myers ( 1954) and Kemp and Kits van Heijningen (1954) were used for the determination of carbohydrate, the animals being first ground with a mixture of 5% trichloracetic acid, containing 0.1% Ag2S04, and the mixture then boiled for 15 min. After cooling and centrifuging, 1 ml of the supernatant liquid was boiled for 61/z min with 3 ml concentrated H2S04 ( 1.84 specific gravity), The intensity of the resulting pink color was then determined by a Beckman D.U.…”
Section: Meteiodsmentioning
confidence: 99%
“…Glycogen was extracted with a 5% (w/v) TCA solution containing 0-1% of silver sulphate and determined by means of a colour reaction (Mendel & Bauch, 1926) which occurs when glycogen is heated with concentrated sulphuric acid (Mendel, Kemp & Myers, 1954;Kemp & Kits van Heijningen, 1954 of the reagent and centrifuged. This supernatant contains the fixed glycogen.…”
Section: Methodsmentioning
confidence: 99%
“…Serum cholesterol and glucose concentration were measured by a spectrophotometric method according to procedures described by Tietz (32) and Mendel et.al. (33), respectively.…”
Section: Biochemical Analysismentioning
confidence: 99%