A Combined Autoradiographic and Immunocytochemical Study of &lt;sup&gt;3&lt;/sup&gt;H-Corticosterone Target Neurons and Catecholamine Neurons in Rat and Mouse Lower Brain Stem

Duncan, Gary E.; Stumpf, Walter E.

doi:10.1159/000124083

Cited by 13 publications

(2 citation statements)

References 16 publications

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“…They should therefore represent an important site of action of glucocorticoid hormones secreted from the adrenal glands. A combined autoradiographic and immunocytochemical study (26) of [3H]corticosterone target neurons and catecholaminergic neurons did not show, but did not exclude, a direct genomic action of corticosterone on catecholaminergic neurons. Previous studies (4,5,8) have shown that the noradrenergic, adrenergic, and 5-hydroxytryptaminergic neurons respond to stress by changing their amine levels and utilization.…”

Section: Discussionmentioning

confidence: 77%

Glucocorticoid receptor immunoreactivity in monoaminergic neurons of rat brain.

Härfstrand¹,

Fuxé²,

Cintra³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

408

178

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A monoclonal antibody against the rat liver glucocorticoid receptor was used in combination with rabbit antibodies against tyrosine hydroxylase, phenylethanolamine N-methyltransferase, and 5-hydroxytryptamine to demonstrate strong glucocorticoid receptor immunoreactivity in large numbers of central monoaminergic nerve cell bodies of the male rat. The receptor immunoreactivity was predominantly located in the nucleus, whereas the tyrosine hydroxylase, phenylethanolamine N-methyltransferase, and 5-hydroxytryptamine were detected mainly in the cytoplasm. The vast majority of the noradrenergic nerve cell bodies of groups Al-A7 and of the 5-hydroxytryptaminergic cell bodies of groups Bl-B9 were found to contain strong glucocorticoid receptor immunoreactivity. The majority of the phenylethanolamine N-methyltransferase-immunoreactive nerve cells of the adrenergic cell groups C1-C3 and of the dorsal subnuclei of the nucleus tractus solitarius in the medulla oblongata were also strongly immunoreactive for glucocorticoid receptor. In the midbrain dopaminergic groups A8-AlO, moderately (A8, A9) to strongly (AlO) glucocorticoid receptor-immunoreactive cells were found, ranging from 40 to 75% of the total population. In the hypothalamic dopaminergic cell groups, all the cells of groups A12 and A14, as well as the majority of the dopaminergic cells of the zona incerta (A13), were found to contain moderate to strong glucocorticoid receptor immunoreactivity, but none of the large dopaminergic cells of the posterior hypothalamus (All) showed such immunoreactivity.

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Section: Discussionmentioning

confidence: 77%

Glucocorticoid receptor immunoreactivity in monoaminergic neurons of rat brain.

Härfstrand¹,

Fuxé²,

Cintra³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

408

178

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“…In vertebrate nervous systems, neural target cells for gonadal steroids, glucocorticoids, mineralocorticoids, and vitamin D3 have been identified (e.g., Pfaff, 1980;Stumpf, Sar, and Clark, 1982;Birmingham, Sar, and Stumpf, 1984;Duncan and Stumpf, 1985;Reul and DeKloet, 1986). A notable feature of steroid hormone receptor distribution in vertebrate nervous systems is that only anatomically restricted subsets of neurons bear receptors for these hormones.…”

Section: Developmental Patterns Of Ecdysteroid Binding In the Manducamentioning

confidence: 99%

Autoradiographic identification of ecdysteroid‐binding cells in the nervous system of the moth Manduca sexta

Fahrbach

Truman

1989

J. Neurobiol.

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The steroid hormone 20-hydroxyecdysone regulates many aspects of nervous system development in the moth Manduca sexta, including stage-specific neuronal morphology and stage-specific neuronal death. We have used steroid hormone autoradiography to study the distribution of cells that concentrate ecdysteroids in the ventral nervous system of this insect. The ligand was [3H]-ponasterone A, a bioactive phytoecdysone. Tissue was examined from three stages of development: the end of larval life (first day of wandering), the end of metamorphosis (pharate adult), and 4-day-old adults. In the abdominal ganglia of wandering larvae and pharate adults, a subset of neurons including both motoneurons and interneurons exhibited a nuclear concentration of radiolabeled hormone. The pattern of binding was reproducible but stage-specific, with a greater proportion of neurons showing binding in the larvae than in pharate adults. No labeled neurons were found in abdominal ganglia from mature (4-day-old) adults. In the case of the pharate adult ganglia, the ecdysteroid receptor content of specific, identified motoneurons was determined. These results are discussed in light of the responses of these neurons to physiological changes in levels of circulating ecdysteroids.

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Dexamethasone and corticosterone receptor sites

et al. 1989

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High resolution light microscopic autoradiography was used, together with regional surveys and combined acridine orange staining, to define in rat hippocampus cellular and subcellular sites of concentration and retention of 3H dexamethasone and to compare the topographic pattern of labeling with that of 3H corticosterone. Nuclear uptake of 3H dexamethasone in the hippocampus is demonstrated for the first time in vivo. With 3H dexamethasone, strongest nuclear radioactive labeling was observed in certain glial cells throughout the hippocampus, followed by strong nuclear labeling in most neurons in area CA1 and in the adjacent dorsolateral subiculum and weak nuclear labeling in granule cells of the dentate gyrus. Neurons in areas CA2, CA3, CA4, and in the dorsomedial subiculum and indusium griseum showed little or no nuclear labeling after 3H dexamethasone. With 3H corticosterone, strongest nuclear labeling was observed in neurons in area CA2 and in the dorsomedial subiculum and indusium griseum, followed by area CA1, then CA3 and CA4; the dentate gyrus contained scattered strongly labeled cells among cells with intermediate nuclear labeling. At the subcellular level, evidence for both nuclear and cytoplasmic accumulation of label was found. The results indicate that dexamethasone and corticosterone have both nuclear and cytoplasmic binding sites and that particular patterns of target cell distribution exist, characteristic for each agent. This suggests a differential regulation of cellular functions for the two compounds. Corticosterone nuclear binding appears to be more extensive and encompasses regions with dexamethasone binding. Whether in certain of these common regions corticosterone binds to the same receptor as dexamethasone, which seems possible, or to different receptors, remains to be clarified.

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A Combined Autoradiographic and Immunocytochemical Study of <sup>3</sup>H-Corticosterone Target Neurons and Catecholamine Neurons in Rat and Mouse Lower Brain Stem

Cited by 13 publications

References 16 publications

Glucocorticoid receptor immunoreactivity in monoaminergic neurons of rat brain.

Glucocorticoid receptor immunoreactivity in monoaminergic neurons of rat brain.

Autoradiographic identification of ecdysteroid‐binding cells in the nervous system of the moth Manduca sexta

Dexamethasone and corticosterone receptor sites

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