A combined DNA-microarray and mechanism-specific toxicity approach with zebrafish embryos to investigate the pollution of river sediments

Kosmehl, Thomas; Otte, Jens C.; Yang, Lixin; Legradi, Jessica; Bluhm, Katharina; Zinsmeister, Christian; Keiter, Steffen; Reifferscheid, Georg; Manz, Werner; Braunbeck, Thomas; Strähle, Uwe; Hollert, Henner

doi:10.1016/j.reprotox.2012.01.005

Cited by 30 publications

(22 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genomics can be indicative of the susceptibility of an organism for a certain chemical or group of chemicals [23] and are more frequently also been used for assessment of complex environmental samples, such as sediments [24]. A DNA microarray is a glass slide or a nylon membrane on which part of the organism's gene sequences (probes) is spotted or synthesised.…”

Section: Genomics-dna Microarray Applicationsmentioning

confidence: 99%

The European technical report on aquatic effect-based monitoring tools under the water framework directive

Carere²,

et al. 2015

Self Cite

View full text Add to dashboard Cite

The Water Framework Directive (WFD), 2000/60/EC, requires an integrated approach to the monitoring and assessment of the quality of surface water bodies. The chemical status assessment is based on compliance with legally binding Environmental Quality Standards (EQSs) for selected chemical pollutants (priority substances) of EU-wide concern. In the context of the mandate for the period 2010 to 2012 of the subgroup Chemical Monitoring and Emerging Pollutants (CMEP) under the Common Implementation Strategy (CIS) for the WFD, a specific task was established for the elaboration of a technical report on aquatic effect-based monitoring tools. The activity was chaired by Sweden and co-chaired by Italy and progressively involved several Member States and stakeholders in an EU-wide drafting group. The main aim of this technical report was to identify potential effect-based tools (e.g. biomarkers and bioassays) that could be used in the context of the different monitoring programmes (surveillance, operational and investigative) linking chemical and ecological status assessment. The present paper summarizes the major technical contents and findings of the report.

show abstract

Section: Genomics-dna Microarray Applicationsmentioning

confidence: 99%

The European technical report on aquatic effect-based monitoring tools under the water framework directive

Carere²,

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…The global detection and analysis of gene expression in this fashion are termed "transcriptomics" Brown and Botstein, 1999;Duggan et al, 1999;Ankley et al, 2006). Transcriptomics can both provide information about the mechanism of action of toxicants and can result in a "genetic signature" which allows for more rapid screening of unknown or suspected toxicants on the basis of their similarity to known toxicants (Hawliczek et al, 2012;Kosmehl et al, 2012). The field of toxicogenomics has progressed rapidly since the application of DNA chips to toxicology was proposed in the late 1990s .…”

Section: Toxicogenomics In Aquatic Toxicology Studiesmentioning

confidence: 99%

Toxicogenomic applications of Chinese rare minnow (Gobiocypris rarus) in aquatic toxicology

Liang

Zha

2016

Comparative Biochemistry and Physiology Part D: Genomics and Pr

View full text Add to dashboard Cite

Rare minnow (Gobiocypris rarus), a Chinese native species, are an excellent emerging model organism for aquatic toxicity testing and chemical safety assessment. "Big data" omics approaches (i.e., genomics, proteomics, and metabolomics) to inform mechanistic toxicology are now applied to studies in rare minnows to better understand toxicity and molecular pathways perturbed by chemicals. This review highlights recent applications of toxicogenomics to study changes in the gene and protein expression profiles in rare minnows in response to chemicals. Here we briefly describe studies that utilized cDNA microarrays in characterization of the cellular effects of rare minnows in single and mixed chemical exposures. Then we compare gel-based proteomics studies in liver of rare minnows following treatment with endocrine disrupting chemicals including 17β-estradiol, 17α-methyltestosterone, pentachlorophenol, and perfluorooctanoic acid. A total of 90 proteins identified in these studies were functionally annotated and categorized. These responsive proteins have roles in biological processes that include metabolism (37.8%), response to oxidation/chemicals (16.7%), signal transduction (11.1%), transport (10%), cytoskeleton (6.7%) and others (17.8%). In addition, recent investigations of endocrine disrupting effects and neurotoxicity of benzotriazole, an emerging contaminant, are summarized. The objective is to continue to enrich genome and protein databases for this species and to integrate molecular datasets to consider temporal effects and complex regulation at the level of the genome and proteome.

show abstract

“…Among these methods is the detection of dioxins and/or dioxin-like PCBs, (anti)oestrogen compounds and (anti)androgen compounds by using CALUX (Chemical Activated LUciferase gene eXpression) assays (Murk et al, 1996). Other molecular approaches include the application of multigene expression profiles (microarrays, at best containing the whole genome of the test species) and proteomics by using the induction profiles of proteins synthesised (e.g., Kosmehl et al, 2012). However, the application of these methods is so far relatively rare in routine testing but this is expected to increase.…”

Section: Sediment Biotestsmentioning

confidence: 99%

Sediment biotesting in the Baltic Sea

Lehtonen¹,

Ahvo²,

Jørgensen³

et al. 2018

TemaNord

View full text Add to dashboard Cite

A combined DNA-microarray and mechanism-specific toxicity approach with zebrafish embryos to investigate the pollution of river sediments

Cited by 30 publications

References 72 publications

The European technical report on aquatic effect-based monitoring tools under the water framework directive

The European technical report on aquatic effect-based monitoring tools under the water framework directive

Toxicogenomic applications of Chinese rare minnow (Gobiocypris rarus) in aquatic toxicology

Sediment biotesting in the Baltic Sea

Contact Info

Product

Resources

About