A Combined Experimental and Modelling Study on Solubility of Calcium Oxalate Monohydrate at Physiologically Relevant pH and Temperatures

Ibis, Fatma; Dhand, Priya; Suleymanli, Sanan; Heijden, A.E.D.M. van der; Kramer, Herman J. M.; Eral, Hüseyin Burak

doi:10.3390/cryst10100924

Cited by 16 publications

(9 citation statements)

References 49 publications

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“…2 shows the SEM images of the CaOx crystals synthesized in aqueous solution with various HA concentrations. The CaOx crystals not containing HA were composed of hexagonal COM crystals which was consistent with the results of previous studies [32,45,48,49]. In addition, it consisted of compact aggregates as a result of the growth of COM crystals on other COM crystals and the main growth of the crystal was on the ( 101) face.…”

Section: Sem Analysissupporting

confidence: 91%

“…Despite the fact that same HA concentrations are used in experiments with deionized water and artificial urine, the solution chemistry is significantly different. Consequently, supersaturations where crystallization occurred in deionized water and artificial urine are different due to combined effect of ionic strength, protonation reactions, and soluble complex formation [49]. The complex solution chemistry of artificial urine solution may overshadow the form altering effect of HA observed in deionized water by increasing the supersaturation across all solutions.…”

Section: Sem Analysismentioning

confidence: 99%

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Elucidating the role of hyaluronic acid in the structure and morphology of calcium oxalate crystals

Polat

Eral

2021

Advanced Powder Technology

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Section: Sem Analysissupporting

confidence: 91%

Section: Sem Analysismentioning

confidence: 99%

Elucidating the role of hyaluronic acid in the structure and morphology of calcium oxalate crystals

Polat

Eral

2021

Advanced Powder Technology

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“…(mol 2 /L 2 ), about twofold lower than in pure water, with pH having the greatest effect (Ibis et al, 2020). The typical pH of the cytosol is $7.2 versus 5.2 in the vacuole, with the trans-Golgi network and multivesicular bodies being about 6.3 (Shen et al, 2013).…”

Section: Potential Mechanism For Profilin Allergenicitymentioning

confidence: 93%

Taro raphide‐associated proteins: Allergens and crystal growth

et al. 2022

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Calcium oxalate raphide crystals are found in bundles in intravacuolar membrane chambers of specialized idioblasts cells of most plant families. Aroid raphides are proposed to cause acridity in crops such as taro (Colocasia esculenta (L.) Schott). Acridity is irritation that causes itchiness and pain when raw/insufficiently cooked tissues are eaten. Since raphides do not always cause acridity and since acridity can be inactivated by cooking and/or protease treatment, it is possible that a toxin or allergen-like compound is associated with the crystals. Using two-dimensional (2D) gel electrophoresis and mass spectrometry (MS) peptide sequencing of selected peptides from purified raphides and taro apex transcriptome sequencing, we showed the presence on the raphides of peptides normally associated with mitochrondria (ATP synthase), chloroplasts (chaperonin $60 kDa), cytoplasm (actin, profilin), and vacuole (V-type ATPase) that indicates a multistage biocrystallation process ending with possible invagination of the tonoplast and addition of mucilage that may be derived from the Golgi. Actin might play a crucial role in the generation of the needle-like raphides.One of the five raphide profilins genes was highly expressed in the apex and had a 17-amino acid insert that significantly increased that profilin's antigenic epitope peak.A second profilin had a 2-amino acid insert and also had a greater B-cell epitope prediction. Taro profilins showed 83% to 92% similarity to known characterized profilins. Further, commercial allergen test strips for hazelnuts, where profilin is a secondary allergen, have potential for screening in a taro germplasm to reduce acridity and during food processing to avoid overcooking. HighlightsAll edible Aroids cause allergic type responses (acridity) thought to be caused by the sharp calcium oxalate raphide crystals. We have shown that raphide-associated proteins could guide biomineralization, and the profilins found are potential allergens.

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“…From this same study, the extreme sensitivity was observed for the presence of melamine upon the formation of calcium oxalate crystals in urine. Fundamental studies of calcium oxalate monohydrate solubility showed increased solubility as the calcium cation was exchanged by sodium, potassium, or magnesium (Ibis et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Effects of millet on calcium oxalate crystal growth and dissolution

Samantha¹,

Vindya²,

Mark³

2023

J. Med. Plants Res.

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The influence of aqueous extract of millet on the crystallization and the dissolution of calcium oxalate (CaOx) crystals were investigated in detail under supersaturated and artificial urinary circumstances in order to understand the effect of the water-soluble components of millet on CaOx kidney stones in humans. To investigate the inhibition effect, different volumes of millet extract were added separately to the combined supersaturated and artificial urinary solutions while the crystal deposition process was monitored by UV-Vis and conductivity measurements. The harvested crystals were characterized by FT-IR, SEM, TGA, XRD and redox titrations so as to determine the structure and the morphology of CaOx crystals formed. Our findings indicate that the crystals obtained from inhibition and dissolution experiments were mainly calcium oxalate monohydrate (COM), the thermodynamically more stable phase. Furthermore, the results show that the millet extract has a promising inhibition effect in the supersaturated solution and in artificial urinary circumstances showing ~34 and ~26% decrease in crystal deposition, respectively, with 50 mL of millet extract compared to control experiments. The dissolution effect of millet extract is far superior to that of the inhibition effect. One gram of COM crystals completely disappeared when the crystals were washed ten times with 300 mL millet extracts. Under the same conditions, the control experiment showed only ~44% dissolution of COM crystals. Further investigations conducted with actual kidney stones under artificial urinary circumstances showed approximately 20% dissolution when washings were carried out using 300-mL portions of millet over one week. This novel finding may shed the light on the possible use of millet as a potential natural antiurolithic remedy to treat CaOx kidney stones in humans.

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A Combined Experimental and Modelling Study on Solubility of Calcium Oxalate Monohydrate at Physiologically Relevant pH and Temperatures

Cited by 16 publications

References 49 publications

Elucidating the role of hyaluronic acid in the structure and morphology of calcium oxalate crystals

Elucidating the role of hyaluronic acid in the structure and morphology of calcium oxalate crystals

Taro raphide‐associated proteins: Allergens and crystal growth

Effects of millet on calcium oxalate crystal growth and dissolution

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