A Combined Patch-Clamp and Electrorotation Study of the Voltage- and Frequency-Dependent Membrane Capacitance Caused by Structurally Dissimilar Lipophilic Anions

Zimmermann, D.; Kiesel, M.; Terpitz, Ulrich; Zhou, Aihua; Reuss, R.; Kraus, Jürgen; Schenk, Wolfdieter A.; Bamberg, Ernst; Sukhorukov, V.L.

doi:10.1007/s00232-007-9090-4

Cited by 29 publications

(18 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Figure C and D represent the CM factor for 3 μm latex beads and HEK293 cells calculated with MyDEP software . Electric properties of HEK293 cells present in the MyDEP database were used . Electric properties values of latex beads used in this paper were previously studied by Suzuki et al.…”

Section: Resultsmentioning

confidence: 99%

Dielectrophoretic cell trapping for improved surface plasmon resonance imaging sensing

et al. 2019

View full text Add to dashboard Cite

The performance of conventional surface plasmon resonance (SPR) biosensors can be limited by the diffusion of the target analyte to the sensor surface. This work presents an SPR biosensor that incorporates an active mass‐transport mechanism based on dielectrophoresis and electroosmotic flow to enhance analyte transport to the sensor surface and reduce the time required for detection. Both these phenomena rely on the generation of AC electric fields that can be tailored by shaping the electrodes that also serve as the SPR sensing areas. Numerical simulations of electric field distribution and microparticle trajectories were performed to choose an optimal electrode design. The proposed design improves on previous work combining SPR with DEP by using face‐to‐face electrodes, rather than a planar interdigitated design. Two different top‐bottom electrode designs were experimentally tested to concentrate firstly latex beads and secondly biological cells onto the SPR sensing area. SPR measurements were then performed by varying the target concentrations. The electrohydrodynamic flow enabled efficient concentration of small objects (3 μm beads, yeasts) onto the SPR sensing area, which resulted in an order of magnitude increased SPR response. Negative dielectrophoresis was also used to concentrate HEK293 cells onto the metal electrodes surrounded by insulating areas, where the SPR response was improved by one order of magnitude.

show abstract

Section: Resultsmentioning

confidence: 99%

Dielectrophoretic cell trapping for improved surface plasmon resonance imaging sensing

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The modeling of cell and particle trapping was performed using COMSOL Multiphysics 5.3 and the add‐on AC/DC and Particle Tracing modules. Simulated particles were configured as single‐shell particles (composed of a cytoplasm surrounded by a thin membrane with human embryonic kidney (HEK) cell properties from the literature (

r_{e x t} = 7.5 μ m, σ_{c p} = 0.533 S / m, ε_{c p} = 71, G_{c m} = 0 S / m^{2}, a n d C_{c m} = 8.5 m F / m^{2}

) . The equation used to implement the single‐shell model with G cm and C cm in COMSOL Multiphysics are presented in the Supporting Information.…”

Section: Methodsmentioning

confidence: 99%

Dielectrophoresis‐assisted creation of cell aggregates under flow conditions using planar electrodes

et al. 2019

View full text Add to dashboard Cite

We present a microfluidic platform allowing dielectrophoresis‐assisted formation of cell aggregates of controlled size and composition under flow conditions. When specific experimental conditions are met, negative dielectrophoresis allows efficient concentration of cells towards electric field minima and subsequent aggregation. This bottom‐up assembly strategy offers several advantages with respect to the targeted application: first, dielectrophoresis offers precise control of spatial cell organization, which can be adjusted by optimizing electrode design. Then, it could contribute to accelerate the establishment of cell‐cell interactions by favoring close contact between neighboring cells. The trapping geometry of our chip is composed of eight electrodes arranged in a circle. Several parameters have been tested in simulations to find the best configurations for trapping in flow. Those configurations have been tested experimentally with both polystyrene beads and human embryonic kidney cells. The final design and experimental setup have been optimized to trap cells and release the created aggregates on demand.

show abstract

“…The hybrid probe hVOS employs FRET between membrane-anchored GFP and a dye, DPA, that partitions into the plasma membrane in a membrane voltage-dependent manner. While this hybrid probe produces the largest reported Δ F/F values among all genetically encoded voltage-sensitive probes, the high concentration of the lipophilic anion DPA has a significant effect on the activity of excitable membranes (Fernández et al, 1983; Chanda et al, 2005; Zimmermann et al, 2008). Thus reduced DPA concentrations were required to obtain action potential responses (Chanda et al, 2005) resulting in much smaller fractional changes (Δ F/F ).…”

Section: Genetically Encoded Sensors Of Membrane Potential Compared Tmentioning

confidence: 99%

Genetically encoded fluorescent sensors of membrane potential

et al. 2008

View full text Add to dashboard Cite

A Combined Patch-Clamp and Electrorotation Study of the Voltage- and Frequency-Dependent Membrane Capacitance Caused by Structurally Dissimilar Lipophilic Anions

Cited by 29 publications

References 50 publications

Dielectrophoretic cell trapping for improved surface plasmon resonance imaging sensing

Dielectrophoretic cell trapping for improved surface plasmon resonance imaging sensing

Dielectrophoresis‐assisted creation of cell aggregates under flow conditions using planar electrodes

Genetically encoded fluorescent sensors of membrane potential

Contact Info

Product

Resources

About