A common framework for EMT and collective cell migration

Campbell, Kyra; Casanova, Jordi

doi:10.1242/dev.139071

Cited by 151 publications

(149 citation statements)

References 102 publications

(130 reference statements)

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“…This has led to the emerging concept of a continuum between the mesenchymal and epithelial state, a topic that recently gathered increasing attention (Campbell and Casanova, 2016; Bernadskaya and Christiaen, 2016). Depending on the context and the morphogenetic processes, cells utilize different states along this continuum.…”

Section: Discussionmentioning

confidence: 99%

Concerted action of neuroepithelial basal shrinkage and active epithelial migration ensures efficient optic cup morphogenesis

Sidhaye

Norden

2017

eLife

101

158

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Organ formation is a multi-scale event that involves changes at the intracellular, cellular and tissue level. Organogenesis often starts with the formation of characteristically shaped organ precursors. However, the cellular mechanisms driving organ precursor formation are often not clear. Here, using zebrafish, we investigate the epithelial rearrangements responsible for the development of the hemispherical retinal neuroepithelium (RNE), a part of the optic cup. We show that in addition to basal shrinkage of RNE cells, active migration of connected epithelial cells into the RNE is a crucial player in its formation. This cellular movement is driven by progressive cell-matrix contacts and actively translocates prospective RNE cells to their correct location before they adopt neuroepithelial fate. Failure of this migration during neuroepithelium formation leads to ectopic determination of RNE cells and consequently impairs optic cup formation. Overall, this study illustrates how spatiotemporal coordination between morphogenic movements and fate determination critically influences organogenesis.DOI: http://dx.doi.org/10.7554/eLife.22689.001

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Section: Discussionmentioning

confidence: 99%

Concerted action of neuroepithelial basal shrinkage and active epithelial migration ensures efficient optic cup morphogenesis

Sidhaye

Norden

2017

eLife

101

158

View full text Add to dashboard Cite

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“…It was proposed that a spectrum exists in cell migratory modes ranging from tight collective to individual cell movement, relating to regulation of EMT (Campbell and Casanova, 2016). Within the spectrum, the cell streaming in a loose collective (exhibited by CVM cells and HC) may be placed at a moderately individual migratory mode.…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of gene expression profiles for several migrating cell types identifies cell migration regulators

Bae

Macabenta

Curtis

et al. 2017

Mechanisms of Development

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Cell migration is an instrumental process that ensures cells are properly positioned to support the specification of distinct tissue types during development. To provide insight, we used fluorescence activated cell sorting (FACS) to isolate two migrating cell types from the Drosophila embryo: caudal visceral mesoderm (CVM) cells, precursors of longitudinal muscles of the gut, and hemocytes (HCs), the Drosophila equivalent of blood cells. ~350 genes were identified from each of the sorted samples using RNA-seq, and in situ hybridization was used to confirm expression within each cell type or, alternatively, within other interacting, co-sorted cell types. To start, the two gene expression profiling datasets were compared to identify cell migration regulators that are potentially generally-acting. 73 genes were present in both CVM cell and HC gene expression profiles, including the transcription factor Zinc finger homeodomain-1 (Zfh1). Comparisons with gene expression profiles of Drosophila border cells that migrate during oogenesis had a more limited overlap, with only the genes neyo (neo) and singed (sn) found to be expressed in border cells as well as CVM cells and HCs, respectively. neo encodes a protein with Zona pellucida domain linked to cell polarity, while sn encodes an actin binding protein. Tissue specific RNAi expression coupled with live in vivo imaging was used to confirm cell-autonomous roles for Zfh1 and Neo in supporting CVM cell migration, whereas previous studies had demonstrated a role for Sn in supporting HC migration. In addition, comparisons were made to migrating cells from vertebrates. Seven genes were found expressed by chick neural crest cells, CVM cells, and HCs including extracellular matrix (ECM) proteins and proteases. In summary, we show that genes shared in common between CVM cells, HCs, and other migrating cell types can help identify regulators of cell migration. Our analyses show that Neo in addition to Zfh1 and Sn studied previously impact cell migration. This study also suggests that modification of the extracellular milieu may be a fundamental requirement for cells that undergo cell streaming migratory behaviors.

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“…This system is advantageous because migrating cells can be observed directly in their normal context through live-imaging of egg chambers, many tools are available to manipulate gene expression, and the genetic regulators of these cells are similar to those in other migratory cell types (Campbell and Casanova, 2016; Hudson and Cooley, 2014; Scarpa and Mayor, 2016). In the ovary, egg chambers are made up of germline cells surrounded by somatic follicle cells, which exist in a single-layer epithelium ((King, 1970) and see Fig.…”

Section: Introductionmentioning

confidence: 99%

A hormonal cue promotes timely follicle cell migration by modulating transcription profiles

Manning

Sheth

Bridges

et al. 2017

Mechanisms of Development

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Cell migration is essential during animal development. In the Drosophila ovary, the steroid hormone ecdysone coordinates nutrient sensing, growth, and the timing of morphogenesis events including border cell migration. To identify downstream effectors of ecdysone signaling, we profiled gene expression in wild-type follicle cells compared to cells expressing a dominant negative Ecdysone receptor or its coactivator Taiman. Of approximately 400 genes that showed differences in expression, we validated 16 candidate genes for expression in border and centripetal cells, and demonstrated that seven responded to ectopic ecdysone activation by changing their transcriptional levels. We found a requirement for seven putative targets in effective cell migration, including two other nuclear hormone receptors, a calcyphosine-encoding gene, and a prolyl hydroxylase. Thus, we identified multiple new genetic regulators modulated at the level of transcription that allow cells to interpret information from the environment and coordinate cell migration in vivo.

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A common framework for EMT and collective cell migration

Cited by 151 publications

References 102 publications

Concerted action of neuroepithelial basal shrinkage and active epithelial migration ensures efficient optic cup morphogenesis

Concerted action of neuroepithelial basal shrinkage and active epithelial migration ensures efficient optic cup morphogenesis

Comparative analysis of gene expression profiles for several migrating cell types identifies cell migration regulators

A hormonal cue promotes timely follicle cell migration by modulating transcription profiles

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