“…In particular, the previous fluorescence based bioanalytic assays have confirmed the binding of thrombin on TBA surfaces, prepared with identical functionalization approach [31]. Furthermore, the additional surface-regeneration step for this aptamer-target system aimed to separate the two biorecognition partners in an efficient manner [49,59] was implemented using NaOH in quartz crystal microbalance technique (QCM, Figure S2), and obtaining saturation levels after 270 nM thrombin, in agreement with previous findings [31] and other publications [60,61].This important step could be further integrated into the SiNW FET concept to increase the lifetime of the microchips by recycling.…”