A Compact Coupling Structure for Diplexers and Filtering Power Dividers

Wu, Yun; Wu, Ruiheng; Wang, Yi

doi:10.2528/pierm18041403

Cited by 8 publications

(12 citation statements)

References 20 publications

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“…Wu et al increased the separation throughput of the taSSAW device to 7.5 mL/h for a blood sample by employing a PDMS−glass hybrid channel. 61 With the increased separation throughput, CTC clusters from prostate cancer patients' samples were also isolated. Compared to other acoustic methods, 62−65 taSSAW allows the use of shorter acoustic wavelengths to improve the separation of smaller particles, such as bacteria and extracellular vesicles.…”

Section: Label-free Cell/particle Separationmentioning

confidence: 99%

Applications of Acoustofluidics in Bioanalytical Chemistry

Huang

2018

Anal. Chem.

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Acoustics has a broad spectrum of applications, ranging from noise cancelation to ultrasonic imaging. In the past decade, there has been increasing interest in developing acoustic-based methods for biological and biomedical applications. This Perspective summarizes the recent progress in applying acoustofluidic methods (i.e., the fusion of acoustics and microfluidics) to bioanalytical chemistry. We describe the concepts of acoustofluidics and how it can be tailored to different types of bioanalytical applications, including sample concentration, fluorescence-activated cell sorting, label-free cell/particle separation, and fluid manipulation. Examples of each application are given, and the benefits and limitations of these methods are discussed. Finally, our perspectives on the directions that developing solutions should take to address the bottlenecks in the acoustofluidic applications in bioanalytical chemistry are presented.

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Section: Label-free Cell/particle Separationmentioning

confidence: 99%

Applications of Acoustofluidics in Bioanalytical Chemistry

Huang

2018

Anal. Chem.

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“…58,61 Recently, supernumerary subunits are suggested to be implicated in forming a megacomplex or respirasome containing complex I, II, III, and IV that increases efficiency of electron transfer. 60,62 We have identified glutathionylation of NDUFB9 and NDUFA9 subunits in complex I (Table 2), which are at the interface with complex III and complex II, respectively (Figure 6C, left). Therefore, glutathionylation at NDUFB9 and NDUFA9 in complex I may affect stability of a mega-complex under oxidative stress.…”

Section: Structural Analyses Of Glutathionylated Cys Residuesmentioning

confidence: 89%

“…Recently, complex I, II, III and their mega-complex structures were analyzed. [59][60][62][63] Our analysis of available structures suggests a potential impact of individual glutathionylation on the megacomplex formation in addition to catalytic functions. Previously, glutathionylation of complex I was investigated, finding glutathionylation at two major Cys residues (C704 and C531) in NDUFS1 (75-kD) and others (e.g.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Identification of Protein Glutathionylation in Cardiomyocytes

2019

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Reactive oxygen species (ROS) are important signaling molecules, but their overproduction is associated with many cardiovascular diseases, including cardiomyopathy. ROS induce various oxidative modifications, among which glutathionylation is one of the significant protein oxidations that occur under oxidative stress. Despite previous efforts, direct and site-specific identification of glutathionylated proteins in cardiomyocytes has been limited. In this report, we used a clickable glutathione approach in a HL-1 mouse cardiomyocyte cell line under exposure to hydrogen peroxide, finding 1,763 glutathionylated peptides with specific Cys modification sites, which include many muscle-specific proteins. Bioinformatic and cluster analyses found 125 glutathionylated proteins, whose mutations or dysfunctions are associated with cardiomyopathy, many of which include sarcomeric structural and contractile proteins, chaperone, and other signaling or regulatory proteins. We further provide functional implication of glutathionylation for several identified proteins, including CSRP3/MLP and complex I, II, and III, by analyzing glutathionylated sites in their structures. Our report establishes a chemoselective method for direct identification of glutathionylated proteins and provides potential target proteins whose glutathionylation may contribute to muscle diseases.

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“…A new design introduced by Wu et al 51 used tilted-angle SAWs and provided significant improvement in the throughput of the acoustic sorting method (Figure 2A). The design increased acoustic energy density by using a hybrid PDMS-glass channel, while simultaneously increasing CTC sorting efficiency to 86% at a throughput of 7.5 mL/h.…”

Section: Acoustofluidic-and Acoustophoretic Cell Sortingmentioning

confidence: 99%

Cell Separations and Sorting

2019

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The development of new methods and technologies for cell separation, sorting, selection, isolation, or enrichment (many times the aforementioned terms are used interchangeably) are becoming more imperative due in part to the wealth of research aimed at addressing issues for the analysis of liquid biopsy targets, such as rare cells, to realize the concept of "precision medicine". Precision medicine is defined as "treatments targeted to the needs of individual patients on the basis of genetic, biomarker, phenotypic, or psychosocial characteristics that distinguish a patient from others with similar clinical presentations." 1 Before full implementation of precision medicine in a hospital or clinical setting, technological discoveries must be realized to assist in the analysis of disease-associated cells enriched from complex samples.Liquid biopsy markers and the cargo that they carry have been shown to be an attractive source of information that can be used to facilitate precise decisions for disease management. These biomarkers include, but are not limited to, rare cells such as circulating tumor cells (CTCs) or cancer stem cells (CSCs), immune cells (i.e., CD8+ T-cells), bacterial and viral cells, cell-free molecules such as cell free DNA (cfDNA), and extracellular vesicles (EVs). Liquid biopsies are attractive because of the minimally invasive nature of the sampling method (i.e., collection of peripheral blood, urine, saliva) to secure relevant biomarkers. These biomarkers can enable precision medicine decisions on managing a variety of diseases, including oncology and nononcology-related diseases. 2,3 Liquid biopsies *

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A Compact Coupling Structure for Diplexers and Filtering Power Dividers

Cited by 8 publications

References 20 publications

Applications of Acoustofluidics in Bioanalytical Chemistry

Applications of Acoustofluidics in Bioanalytical Chemistry

Proteomic Identification of Protein Glutathionylation in Cardiomyocytes

Cell Separations and Sorting

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