A compact integrated microfluidic oxygenator with high gas exchange efficiency and compatibility for long-lasting endothelialization

Lachaux, Julie; Hwang, Gilgueng; Arouche, Nassim; Naserian, Sina; Harouri, A.; Lotito, Valeria; Casari, Caterina; Lok, Thévy; Menager, Jean Baptiste; Issard, Justin; Guihaire, Julien; Denis, Cécile V.; Lenting, Peter J.; Barakat, Abdul I.; Uzan, Georges; Mercier, Olaf; Haghiri‐Gosnet, A. M.

doi:10.1039/d1lc00356a

Cited by 17 publications

(14 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a second step, the tumor physiopathology will be reproduced by culturing endothelial cells in the external channel until confluence. To ensure optimal cell attachment, the surface of the device will be suitably treated, for instance by coating it with a mixture of albumin and heparin, as described previously [63]. Then, their barrier function can be modulated by exposing the cells to different cytokines to mimic the increased permeability and retention (EPR) effect observed in a variety of tumors.…”

Section: Discussionmentioning

confidence: 99%

Fabrication of high aspect ratio microfluidic devices for long term in vitro culture of 3D tumor models

Ugrinic

Decanini

Bidan

et al. 2023

Microelectronic Engineering

Self Cite

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Fabrication of high aspect ratio microfluidic devices for long term in vitro culture of 3D tumor models

Ugrinic

Decanini

Bidan

et al. 2023

Microelectronic Engineering

Self Cite

View full text Add to dashboard Cite

“…The other limitation of our study is that the vessel model was produced under a static condition. While the advantage of the static culture is that it does not cause any mechanotransduction associated with shear stress on the endothelial cells during cell culture, the real in vivo situation always involved shear stress [ 87 ]. Further study will be needed to investigate the effect of different cell culture conditions.…”

Section: Discussionmentioning

confidence: 99%

Effect of Photo-Mediated Ultrasound Therapy on Nitric Oxide and Prostacyclin from Endothelial Cells

Karthikesh

Singh

et al. 2022

Applied Sciences

View full text Add to dashboard Cite

Several studies have investigated the effect of photo-mediated ultrasound therapy (PUT) on the treatment of neovascularization. This study explores the impact of PUT on the release of the vasoactive agents nitric oxide (NO) and prostacyclin (PGI2) from the endothelial cells in an in vitro blood vessel model. In this study, an in vitro vessel model containing RF/6A chorioretinal endothelial cells was used. The vessels were treated with ultrasound-only (0.5, 1.0, 1.5 and 2.0 MPa peak negative pressure at 0.5 MHz with 10% duty cycle), laser-only (5, 10, 15 and 20 mJ/cm2 at 532 nm with a pulse width of 5 ns), and synchronized laser and ultrasound (PUT) treatments. Passive cavitation detection was used to determine the cavitation activities during treatment. The levels of NO and PGI2 generally increased when the applied ultrasound pressure and laser fluence were low. The increases in NO and PGI2 levels were significantly reduced by 37.2% and 42.7%, respectively, from 0.5 to 1.5 MPa when only ultrasound was applied. The increase in NO was significantly reduced by 89.5% from 5 to 20 mJ/cm2, when only the laser was used. In the PUT group, for 10 mJ/cm2 laser fluence, the release of NO decreased by 76.8% from 0.1 to 1 MPa ultrasound pressure. For 0.5 MPa ultrasound pressure in the PUT group, the release of PGI2 started to decrease by 144% from 15 to 20 mJ/cm2 laser fluence. The decreases in NO and PGI2 levels coincided with the increased cavitation activities in each group. In conclusion, PUT can induce a significant reduction in the release of NO and PGI2 in comparison with ultrasound-only and laser-only treatments.

show abstract

“…These microfluidic artificial lungs (µALs) can exhibit µm-tall artificial capillaries, thereby increasing gas exchange efficiency and achieving physiologic blood flow paths, potentially increasing biocompatibility compared to current alternatives. To date, our and other research groups have demonstrated small-scale, single-layer two-dimensional (2D) µALs with record gas exchange efficiency [10,11], biomimetic blood flow networks [10][11][12][13][14][15][16][17][18], surface coatings to reduce protein and platelet deposition and increase lifetime [15,[19][20][21][22], and have also shown that endothelial cells can be grown in microfluidic blood flow networks and significantly reduce thrombus area [16,[23][24][25]. Manufacturing techniques have been demonstrated to increase the blood flow capacity of µAls and move these devices towards clinical application [11,14,16,[26][27][28][29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

A Parametric Analysis of Capillary Height in Single-Layer, Small-Scale Microfluidic Artificial Lungs

et al. 2022

View full text Add to dashboard Cite

Microfluidic artificial lungs (μALs) are being investigated for their ability to closely mimic the size scale and cellular environment of natural lungs. Researchers have developed μALs with small artificial capillary diameters (10–50 µm; to increase gas exchange efficiency) and with large capillary diameters (~100 µm; to simplify design and construction). However, no study has directly investigated the impact of capillary height on μAL properties. Here, we use Murray’s law and the Hagen-Poiseuille equation to design single-layer, small-scale μALs with capillary heights between 10 and 100 µm. Each µAL contained two blood channel types: capillaries for gas exchange; and distribution channels for delivering blood to/from capillaries. Three designs with capillary heights of 30, 60, and 100 µm were chosen for further modeling, implementation and testing with blood. Flow simulations were used to validate and ensure equal pressures. Designs were fabricated using soft lithography. Gas exchange and pressure drop were tested using whole bovine blood. All three designs exhibited similar pressure drops and gas exchange; however, the μAL with 60 µm tall capillaries had a significantly higher wall shear rate (although physiologic), smaller priming volume and smaller total blood contacting surface area than the 30 and 100 µm designs. Future μAL designs may need to consider the impact of capillary height when optimizing performance.

show abstract

A compact integrated microfluidic oxygenator with high gas exchange efficiency and compatibility for long-lasting endothelialization

Abstract: A novel microfluidic architecture allowing simple parallel stacking of the different 4 inch circular units of curved blood capillaries covering a maximal surface area with high compacity is proposed for blood oxygenation at high flow rate.

Cited by 17 publications

References 28 publications

Fabrication of high aspect ratio microfluidic devices for long term in vitro culture of 3D tumor models

Fabrication of high aspect ratio microfluidic devices for long term in vitro culture of 3D tumor models

Effect of Photo-Mediated Ultrasound Therapy on Nitric Oxide and Prostacyclin from Endothelial Cells

A Parametric Analysis of Capillary Height in Single-Layer, Small-Scale Microfluidic Artificial Lungs

Contact Info

Product

Resources

About